KEL3_YEAST
ID KEL3_YEAST Reviewed; 651 AA.
AC Q08979; D6W3A7;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Kelch repeat-containing protein 3;
GN Name=KEL3; OrderedLocusNames=YPL263C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- MISCELLANEOUS: Present with 4010 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z73619; CAA97998.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11173.1; -; Genomic_DNA.
DR PIR; S65296; S65296.
DR RefSeq; NP_015060.1; NM_001184077.1.
DR AlphaFoldDB; Q08979; -.
DR SMR; Q08979; -.
DR BioGRID; 35950; 52.
DR DIP; DIP-3926N; -.
DR IntAct; Q08979; 2.
DR MINT; Q08979; -.
DR STRING; 4932.YPL263C; -.
DR iPTMnet; Q08979; -.
DR MaxQB; Q08979; -.
DR PaxDb; Q08979; -.
DR PRIDE; Q08979; -.
DR EnsemblFungi; YPL263C_mRNA; YPL263C; YPL263C.
DR GeneID; 855865; -.
DR KEGG; sce:YPL263C; -.
DR SGD; S000006184; KEL3.
DR VEuPathDB; FungiDB:YPL263C; -.
DR eggNOG; KOG1230; Eukaryota.
DR GeneTree; ENSGT00390000003374; -.
DR HOGENOM; CLU_008722_0_0_1; -.
DR InParanoid; Q08979; -.
DR OMA; GHRMTYY; -.
DR BioCyc; YEAST:G3O-34146-MON; -.
DR PRO; PR:Q08979; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q08979; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR Gene3D; 2.120.10.80; -; 1.
DR InterPro; IPR025183; DUF4110.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR011498; Kelch_2.
DR Pfam; PF13422; DUF4110; 1.
DR Pfam; PF07646; Kelch_2; 1.
DR SUPFAM; SSF117281; SSF117281; 1.
PE 1: Evidence at protein level;
KW Kelch repeat; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..651
FT /note="Kelch repeat-containing protein 3"
FT /id="PRO_0000119098"
FT REPEAT 94..148
FT /note="Kelch 1"
FT REPEAT 150..199
FT /note="Kelch 2"
FT REPEAT 208..259
FT /note="Kelch 3"
FT REPEAT 262..310
FT /note="Kelch 4"
FT REPEAT 334..383
FT /note="Kelch 5"
FT REGION 1..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 369..392
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 406..440
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 511..572
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 10..42
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 410..440
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 518..556
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 557..572
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 47
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198"
SQ SEQUENCE 651 AA; 75433 MW; FD0C665A6AB30843 CRC64;
MAKKNKKDKE AKKARAELKN QKNQKKQEKK FQKNKNKSLN GEEDDESDQD LDEILSSFSK
KQIELEHVDI TSVEKPSCRT HPLMFANPQH NKHELFIFGG EFTDPETKLT HFYNDLYSYS
IKNNSWKKYV SQNAPLPRSS AAVAVHPSGI ALLHGGEFSS PKQSKFYHYS DTWLFDCVER
KFTKLEFGGR DSSPSARSGH RIIAWKNYFI LFGGFRDLGN GQTSYLNDLW CFDISTYKWT
KLETNSKPDA RSGHCFIPTD NSAILMGGYC KIIAKNNKNL MKGKILNDAW KLNLTPDPKK
WQWEKLKNFK NQPSPRVGYS FNLWKQNKSV AFGGVYDLQE TEESLESVFY NDLYMFHLEL
NKWSKLRIKP QRQTNSKNSP ATSKRKSNKD QEKELQDLLN SILAKSNLND DDDDNDDNST
TGPNSIDDDE DNEDDSDLDN QEDITISNQL PHPRFNAATC VVGDSLFIYS GVWELGEKDY
PINSFYSIDL NKLDGVKVYW EDLSAIEEAK RLGDRDSDED EFEYEDDEED EDDGEEEQDA
GPLEGDEDEE SESDDDKQAQ MEIPDERSWL PHPKPFETLR AFYLREGANF LTWSISNNRN
LKGKQLKTKS FELCEDRWWE RRDQVTLEEE RLEDTGGIIE RDTTTKPSKR R