KEN_DROME
ID KEN_DROME Reviewed; 601 AA.
AC O77459;
DT 02-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Transcription factor Ken;
DE AltName: Full=Protein Ken and Barbie;
GN Name=ken; ORFNames=CG5575;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=10349629; DOI=10.1016/s0925-4773(98)00183-x;
RA Kuehnlein R.P., Chen C.-K., Schuh R.;
RT "A transcription unit at the ken and barbie gene locus encodes a novel
RT Drosophila zinc finger protein.";
RL Mech. Dev. 79:161-164(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX PubMed=10191082; DOI=10.1006/geno.1999.5746;
RA Lukacsovich T., Asztalos Z., Juni N., Awano W., Yamamoto D.;
RT "The Drosophila melanogaster 60A chromosomal division is extremely dense
RT with functional genes: their sequences, genomic organization, and
RT expression.";
RL Genomics 57:43-56(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=14518006; DOI=10.1002/arch.10105;
RA Lukacsovich T., Yuge K., Awano W., Asztalos Z., Kondo S., Juni N.,
RA Yamamoto D.;
RT "The ken and barbie gene encoding a putative transcription factor with a
RT BTB domain and three zinc finger motifs functions in terminalia development
RT of Drosophila.";
RL Arch. Insect Biochem. Physiol. 54:77-94(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=16401426; DOI=10.1016/j.cub.2005.11.033;
RA Arbouzova N.I., Bach E.A., Zeidler M.P.;
RT "Ken & barbie selectively regulates the expression of a subset of Jak/STAT
RT pathway target genes.";
RL Curr. Biol. 16:80-88(2006).
CC -!- FUNCTION: Transcription factor required for terminalia development.
CC Negative regulator of the JAK/STAT pathway: represses JAK/STAT-
CC dependent expression of ventral veins lacking (vvl) in the posterior
CC spiracles. {ECO:0000269|PubMed:14518006, ECO:0000269|PubMed:16401426}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed from stage 5 in two rather faint stripes
CC at positions of 64% (anterior domain; AD) and 17% (posterior domain;
CC PD) egg length. During early gastrulation, at stage 6, these two
CC stripes become more evident and detectable at the region posterior to
CC the cephalic furrow and in the hindgut primordium. The AD disappears as
CC gastrulation proceeds, while the PD remains. At stage 15, the AD
CC appears again in the foregut, and PD expression in the hindgut and anal
CC pad. In imaginal disks, it is ubiquitously expressed in both males and
CC females in genital and eye-antennal disks. Not expressed in the brain.
CC In genital disks, it is expressed along the margin of the anterior
CC bulbus in males, while in females it is expressed in the posterior
CC compartment along the anterior-posterior border, with medial expansion
CC in the most posterior region. {ECO:0000269|PubMed:10349629,
CC ECO:0000269|PubMed:14518006, ECO:0000269|PubMed:16401426}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically. Highly
CC expressed in embryos and pupae, and at lower level in larvae. In
CC adults, it is expressed at higher level in females.
CC {ECO:0000269|PubMed:10349629, ECO:0000269|PubMed:14518006,
CC ECO:0000269|PubMed:16401426}.
CC -!- DISRUPTION PHENOTYPE: Flies show low mating success and reduced
CC copulation duration. Men and female genitalia often remain inside the
CC body, and genitalia and analia are missing in some homozygous flies.
CC These flies are named after the famous dolls who also lack these
CC 'features'. {ECO:0000269|PubMed:10349629}.
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DR EMBL; AJ012576; CAA10062.1; -; mRNA.
DR EMBL; AB010260; BAA32683.1; -; mRNA.
DR EMBL; AB010261; BAA32687.1; -; Genomic_DNA.
DR EMBL; AE013599; AAF47084.1; -; Genomic_DNA.
DR EMBL; AF181647; AAD55433.1; -; mRNA.
DR PIR; T00119; T00119.
DR RefSeq; NP_523833.1; NM_079109.3.
DR AlphaFoldDB; O77459; -.
DR SMR; O77459; -.
DR BioGRID; 63378; 12.
DR DIP; DIP-22018N; -.
DR IntAct; O77459; 3.
DR STRING; 7227.FBpp0072038; -.
DR PaxDb; O77459; -.
DR PRIDE; O77459; -.
DR EnsemblMetazoa; FBtr0072129; FBpp0072038; FBgn0011236.
DR GeneID; 37785; -.
DR KEGG; dme:Dmel_CG5575; -.
DR CTD; 37785; -.
DR FlyBase; FBgn0011236; ken.
DR VEuPathDB; VectorBase:FBgn0011236; -.
DR eggNOG; KOG1721; Eukaryota.
DR HOGENOM; CLU_019233_0_0_1; -.
DR InParanoid; O77459; -.
DR OMA; QQFVYQW; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; O77459; -.
DR Reactome; R-DME-210671; Transcriptional repression by nuclear factors.
DR BioGRID-ORCS; 37785; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 37785; -.
DR PRO; PR:O77459; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0011236; Expressed in second segment of antenna (Drosophila) and 52 other tissues.
DR Genevisible; O77459; DM.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IMP:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0045497; P:female analia development; IMP:UniProtKB.
DR GO; GO:0030540; P:female genitalia development; IMP:UniProtKB.
DR GO; GO:0045496; P:male analia development; IMP:UniProtKB.
DR GO; GO:0030539; P:male genitalia development; IMP:UniProtKB.
DR GO; GO:0046426; P:negative regulation of receptor signaling pathway via JAK-STAT; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00651; BTB; 1.
DR SMART; SM00225; BTB; 1.
DR SMART; SM00355; ZnF_C2H2; 3.
DR SUPFAM; SSF54695; SSF54695; 1.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS50097; BTB; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Repressor; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..601
FT /note="Transcription factor Ken"
FT /id="PRO_0000046925"
FT DOMAIN 33..101
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT ZN_FING 500..522
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 528..551
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 567..590
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 188..276
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 331..365
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 471..491
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 193..220
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 221..246
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 260..276
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 601 AA; 67033 MW; C9AAA437DAD48FE8 CRC64;
MKEFQRMLML QYSKHGECIL KEIGAAFRGE HPADLTIVCE NKVKLHAHKL VLAAASPLIR
NLLEDTHLSD CSTTVYFPDV NATYFKFLLD FLYSGQTCIT SRDVNYLHDL LLLLQIKSDS
WKTTDSAYLS SKCGGLRDRA DRRKQQYTSP QNLEPDQTLK YEVDSVDESR NAADFSSAFN
SNDNCESAAE CERSGGHNNK EEDEDDCTHK DNKSDKDTDE IVNLSNAPPS GTSGSNSNIS
TSSNHQQQQH HHHHHHNHNN NNNNNNNNSS SSTINPVNLS LDLRTKSENS ASRTLGSGSD
HSGIDLAVTA SESTKRKGLF FDSHKDVMKP LSDGSDINSS PENYVVTPHR KRRPGFHNTQ
SDNQPFTSYP HSLLEELRLA KSTTSPISGF GSEKNMLAHL EDGALNGDTL TPDRKHLLEA
QRNRAQSPEI PMHLGPQFVY QWQSNQNAAM SAMPNLQSRL SSLSHISLNL DHPEGRSGSA
SGSGANLAGS NTHASSVREY RCEYCGKQFG MSWNLKTHLR VHTGEKPFAC RLCVAMFKQK
AHLLKHLCSV HRNVITTTNG ADTENRYSCC FCSMCFESVQ ELVRHLSGHH NNLLLTKNLR
E
