KERA_DANRE
ID KERA_DANRE Reviewed; 348 AA.
AC Q5RI43; Q0PZF1;
DT 22-NOV-2017, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Keratocan {ECO:0000303|PubMed:17965408};
DE Flags: Precursor;
GN Name=kera {ECO:0000303|PubMed:17965408};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000312|Proteomes:UP000000437};
RN [1] {ECO:0000312|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2] {ECO:0000312|EMBL:AAI15059.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000312|EMBL:ABG72686.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 3-348, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, GLYCOSYLATION, AND DISRUPTION PHENOTYPE.
RX PubMed=17965408; DOI=10.1074/jbc.m707656200;
RA Yeh L.K., Liu C.Y., Chien C.L., Converse R.L., Kao W.W., Chen M.S.,
RA Hu F.R., Hsieh F.J., Wang I.J.;
RT "Molecular analysis and characterization of zebrafish keratocan (zKera)
RT gene.";
RL J. Biol. Chem. 283:506-517(2008).
CC -!- FUNCTION: May be important in developing and maintaining corneal
CC transparency and for the structure of the stromal matrix.
CC {ECO:0000250|UniProtKB:O60938}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix {ECO:0000305|PubMed:17965408}.
CC -!- TISSUE SPECIFICITY: Expressed in eye, where it is found in the corneal
CC epithelial layer and to a lesser extent in the stromal layer (at
CC protein level). {ECO:0000269|PubMed:17965408}.
CC -!- DEVELOPMENTAL STAGE: At 2-3 days post-fertilization, detected in eye,
CC forebrain, midbrain, hindbrain and anterior spinal cord.
CC {ECO:0000269|PubMed:17965408}.
CC -!- PTM: Glycosylated. Contains keratan sulfate chains.
CC {ECO:0000269|PubMed:17965408}.
CC -!- DISRUPTION PHENOTYPE: Morpolino knockdown of the protein results in
CC increased apoptosis in eye, forebrain and midbrain, and reduced
CC survival rate in embryos. {ECO:0000269|PubMed:17965408}.
CC -!- SIMILARITY: Belongs to the small leucine-rich proteoglycan (SLRP)
CC family. SLRP class II subfamily. {ECO:0000305}.
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DR EMBL; BX322608; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC115058; AAI15059.1; -; mRNA.
DR EMBL; DQ667686; ABG72686.1; -; Genomic_DNA.
DR RefSeq; NP_001020719.1; NM_001025548.2.
DR RefSeq; XP_005164822.1; XM_005164765.2.
DR AlphaFoldDB; Q5RI43; -.
DR SMR; Q5RI43; -.
DR STRING; 7955.ENSDARP00000073976; -.
DR PaxDb; Q5RI43; -.
DR Ensembl; ENSDART00000079521; ENSDARP00000073976; ENSDARG00000056938.
DR GeneID; 567017; -.
DR KEGG; dre:567017; -.
DR CTD; 11081; -.
DR ZFIN; ZDB-GENE-041210-165; kera.
DR eggNOG; KOG0619; Eukaryota.
DR GeneTree; ENSGT00940000158968; -.
DR HOGENOM; CLU_000288_186_4_1; -.
DR InParanoid; Q5RI43; -.
DR OMA; MECFCPP; -.
DR OrthoDB; 826997at2759; -.
DR PhylomeDB; Q5RI43; -.
DR TreeFam; TF334562; -.
DR Reactome; R-DRE-2022854; Keratan sulfate biosynthesis.
DR Reactome; R-DRE-2022857; Keratan sulfate degradation.
DR PRO; PR:Q5RI43; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 4.
DR Bgee; ENSDARG00000056938; Expressed in head and 19 other tissues.
DR ExpressionAtlas; Q5RI43; baseline and differential.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR000372; LRRNT.
DR Pfam; PF13855; LRR_8; 3.
DR Pfam; PF01462; LRRNT; 1.
DR SMART; SM00369; LRR_TYP; 5.
DR SMART; SM00013; LRRNT; 1.
DR PROSITE; PS51450; LRR; 8.
PE 1: Evidence at protein level;
KW Disulfide bond; Extracellular matrix; Glycoprotein; Leucine-rich repeat;
KW Reference proteome; Repeat; Secreted; Sensory transduction; Signal; Vision.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..348
FT /note="Keratocan"
FT /id="PRO_5010844546"
FT DOMAIN 40..69
FT /note="LRRNT"
FT /evidence="ECO:0000255"
FT REPEAT 70..92
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 93..118
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 119..140
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 141..163
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 165..189
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 190..213
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 215..234
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 235..260
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 262..280
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT REPEAT 281..303
FT /note="LRR 10"
FT /evidence="ECO:0000255"
FT CARBOHYD 92
FT /note="N-linked (GlcNAc...) (keratan sulfate) asparagine"
FT /evidence="ECO:0000250|UniProtKB:O42235"
FT CARBOHYD 259
FT /note="N-linked (GlcNAc...) (keratan sulfate) asparagine"
FT /evidence="ECO:0000250|UniProtKB:O42235"
FT CARBOHYD 297
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 41..47
FT /evidence="ECO:0000250|UniProtKB:P21793"
FT DISULFID 45..57
FT /evidence="ECO:0000250|UniProtKB:P21793"
FT DISULFID 302..339
FT /evidence="ECO:0000250|UniProtKB:P21793"
SQ SEQUENCE 348 AA; 38938 MW; 2872AB178511645F CRC64;
MSRLNLTMEV LLVAFVAVFL TSQVHSDEIP YEHLMSQLQA CPKECNCPPN FPNAVYCDNK
GLKSIPVIPP YTWYLYLQNN LIDVLSANAL RNATQLKWIN LNRNKITTEG LEVDALRAMS
NLVHLYMEDN LLSSIPSPLP AKLEQLRLSR NKISKIPPGV FSGMGHLTLL DLQSNKLQDD
AVTEVSLKGL NNLIQINLAK NQLNSMPLGL PPTTTQIFLD GNNIEKIPAE YFKGLPKVAS
LRLNRNKLAN GGIPKNVFNL SSILDLQLSH NQLTEVPVIS SGLEHLHLDH NKIKSVNSSD
ICPPGVLDDH LEEKSPRLRY LRLDGNEIQP PIPRELMTCF RLLRAVVI
