ARAA_GEOKA
ID ARAA_GEOKA Reviewed; 496 AA.
AC Q5KYP7;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2006, sequence version 2.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=L-arabinose isomerase {ECO:0000255|HAMAP-Rule:MF_00519};
DE EC=5.3.1.4 {ECO:0000255|HAMAP-Rule:MF_00519};
GN Name=araA {ECO:0000255|HAMAP-Rule:MF_00519}; OrderedLocusNames=GK1904;
OS Geobacillus kaustophilus (strain HTA426).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus;
OC Geobacillus thermoleovorans group.
OX NCBI_TaxID=235909;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HTA426;
RX PubMed=15576355; DOI=10.1093/nar/gkh970;
RA Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H.,
RA Matsui S., Uchiyama I.;
RT "Thermoadaptation trait revealed by the genome sequence of thermophilic
RT Geobacillus kaustophilus.";
RL Nucleic Acids Res. 32:6292-6303(2004).
CC -!- FUNCTION: Catalyzes the conversion of L-arabinose to L-ribulose.
CC {ECO:0000255|HAMAP-Rule:MF_00519}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-L-arabinopyranose = L-ribulose; Xref=Rhea:RHEA:14821,
CC ChEBI:CHEBI:16880, ChEBI:CHEBI:40886; EC=5.3.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00519};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00519};
CC Note=Binds 1 Mn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_00519};
CC -!- PATHWAY: Carbohydrate degradation; L-arabinose degradation via L-
CC ribulose; D-xylulose 5-phosphate from L-arabinose (bacterial route):
CC step 1/3. {ECO:0000255|HAMAP-Rule:MF_00519}.
CC -!- SIMILARITY: Belongs to the arabinose isomerase family.
CC {ECO:0000255|HAMAP-Rule:MF_00519}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD76189.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; BA000043; BAD76189.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_041467871.1; NC_006510.1.
DR PDB; 4R1O; X-ray; 2.40 A; A/B/C/D/E/F=1-496.
DR PDB; 4R1P; X-ray; 2.30 A; A/B/C/D/E/F=1-496.
DR PDB; 4R1Q; X-ray; 2.25 A; A/B/C/D/E/F=1-496.
DR PDB; 7CHL; X-ray; 3.40 A; A/B/C/D/E/F/G/H/I/J/K/L=133-284, A/B/C/D/E/F/G/H/I/J/K/L=384-496.
DR PDB; 7CX7; X-ray; 2.49 A; A/B/C/D/E/F=33-132, A/B/C/D/E/F=285-342, A/B/C/D/E/F=487-496.
DR PDB; 7CXO; X-ray; 3.20 A; A/B/C/D/E/F=133-284, A/B/C/D/E/F=384-496.
DR PDB; 7CYY; X-ray; 2.60 A; A/B/C/D/E/F=33-132, A/B/C/D/E/F=285-342, A/B/C/D/E/F=487-496.
DR PDBsum; 4R1O; -.
DR PDBsum; 4R1P; -.
DR PDBsum; 4R1Q; -.
DR PDBsum; 7CHL; -.
DR PDBsum; 7CX7; -.
DR PDBsum; 7CXO; -.
DR PDBsum; 7CYY; -.
DR AlphaFoldDB; Q5KYP7; -.
DR SMR; Q5KYP7; -.
DR STRING; 235909.GK1904; -.
DR EnsemblBacteria; BAD76189; BAD76189; GK1904.
DR KEGG; gka:GK1904; -.
DR PATRIC; fig|235909.7.peg.2042; -.
DR eggNOG; COG2160; Bacteria.
DR HOGENOM; CLU_045663_0_0_9; -.
DR OMA; HMLEICP; -.
DR BRENDA; 5.3.1.4; 8138.
DR UniPathway; UPA00145; UER00565.
DR Proteomes; UP000001172; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0008733; F:L-arabinose isomerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0019569; P:L-arabinose catabolic process to xylulose 5-phosphate; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.10940; -; 1.
DR HAMAP; MF_00519; Arabinose_Isome; 1.
DR InterPro; IPR024664; Ara_Isoase_C.
DR InterPro; IPR038583; AraA_N_sf.
DR InterPro; IPR004216; Fuc/Ara_isomerase_C.
DR InterPro; IPR009015; Fucose_isomerase_N/cen_sf.
DR InterPro; IPR003762; Lara_isomerase.
DR PANTHER; PTHR38464; PTHR38464; 1.
DR Pfam; PF11762; Arabinose_Iso_C; 1.
DR Pfam; PF02610; Arabinose_Isome; 1.
DR PIRSF; PIRSF001478; L-ara_isomerase; 1.
DR SUPFAM; SSF50443; SSF50443; 1.
DR SUPFAM; SSF53743; SSF53743; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Arabinose catabolism; Carbohydrate metabolism; Isomerase;
KW Manganese; Metal-binding; Reference proteome.
FT CHAIN 1..496
FT /note="L-arabinose isomerase"
FT /id="PRO_0000259339"
FT BINDING 306
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00519"
FT BINDING 331
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00519"
FT BINDING 348
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00519"
FT BINDING 447
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00519"
FT STRAND 8..14
FT /evidence="ECO:0007829|PDB:4R1Q"
FT HELIX 21..38
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 40..46
FT /evidence="ECO:0007829|PDB:7CX7"
FT STRAND 48..50
FT /evidence="ECO:0007829|PDB:4R1Q"
FT HELIX 57..69
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 73..82
FT /evidence="ECO:0007829|PDB:4R1Q"
FT HELIX 86..89
FT /evidence="ECO:0007829|PDB:4R1Q"
FT HELIX 90..95
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 100..104
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 107..110
FT /evidence="ECO:0007829|PDB:4R1Q"
FT TURN 113..115
FT /evidence="ECO:0007829|PDB:4R1Q"
FT HELIX 118..123
FT /evidence="ECO:0007829|PDB:4R1Q"
FT HELIX 126..139
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 145..149
FT /evidence="ECO:0007829|PDB:4R1Q"
FT HELIX 154..174
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 177..182
FT /evidence="ECO:0007829|PDB:4R1Q"
FT HELIX 195..202
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 205..209
FT /evidence="ECO:0007829|PDB:4R1Q"
FT HELIX 211..219
FT /evidence="ECO:0007829|PDB:4R1Q"
FT HELIX 223..236
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 237..239
FT /evidence="ECO:0007829|PDB:4R1Q"
FT HELIX 241..243
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 244..247
FT /evidence="ECO:0007829|PDB:4R1Q"
FT HELIX 248..269
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 274..276
FT /evidence="ECO:0007829|PDB:4R1Q"
FT HELIX 291..298
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 302..305
FT /evidence="ECO:0007829|PDB:4R1Q"
FT HELIX 309..322
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 325..336
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 343..347
FT /evidence="ECO:0007829|PDB:4R1Q"
FT HELIX 354..356
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 357..359
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 362..365
FT /evidence="ECO:0007829|PDB:4R1Q"
FT TURN 369..372
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 377..380
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 386..395
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 397..410
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 423..430
FT /evidence="ECO:0007829|PDB:4R1Q"
FT HELIX 432..441
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 446..453
FT /evidence="ECO:0007829|PDB:4R1Q"
FT HELIX 456..466
FT /evidence="ECO:0007829|PDB:4R1Q"
FT STRAND 469..474
FT /evidence="ECO:0007829|PDB:4R1Q"
FT HELIX 479..493
FT /evidence="ECO:0007829|PDB:4R1Q"
SQ SEQUENCE 496 AA; 56105 MW; DE7429FB763DFDF9 CRC64;
MLSLRPYEFW FVTGSQHLYG EEALKQVEEH SRIMVNEWNR DSVFPFPFVF KSVVTTPEEI
RRVCLEANAS EQCAGVVTWM HTFSPAKMWI GGLLELRKPL LHLHTQFNRD IPWDSIDMDF
MNLNQSAHGD REYGFIGARM GVARKVVVGH WEDPEVRERL AKWMRTAVAF AESRNLKVAR
FGDNMREVAV TEGDKVGAQI QFGWSVNGYG IGDLVQYIRD VSEQKVNELL DEYEELYDIV
PAGRQEGPVR ESIREQARIE LGLKAFLQDG NFTAFTTTFE DLHGMKQLPG LAVQRLMAEG
YGFGGEGDWK TAALVRLMKV MADGKGTSFM EDYTYHFEPG NELILGAHML EVCPTIAATR
PRVEVHPLSI GGKEDPARLV FDGGEGAAVN ASLIDLGHRF RLIVNEVDAV KPEHDMPKLP
VARILWKPRP SLRDSAEAWI LAGGAHHTCF SFAVTTEQLQ DFAEMAGIEC VVINEHTSVS
SFKNELKWNE VFWRGR
