KGD4_BOVIN
ID KGD4_BOVIN Reviewed; 103 AA.
AC P82908; Q3SYR1;
DT 02-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 04-APR-2006, sequence version 2.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Alpha-ketoglutarate dehydrogenase component 4 {ECO:0000250|UniProtKB:Q9CQX8};
GN Name=MRPS36; Synonyms=KGD4 {ECO:0000250|UniProtKB:Q9CQX8};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913 {ECO:0000305};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Rumen;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP PROTEIN SEQUENCE OF 61-78 AND 88-103, AND SUBCELLULAR LOCATION.
RC TISSUE=Liver;
RX PubMed=11279123; DOI=10.1074/jbc.m100727200;
RA Koc E.C., Burkhart W., Blackburn K., Moseley A., Spremulli L.L.;
RT "The small subunit of the mammalian mitochondrial ribosome: identification
RT of the full complement of ribosomal proteins present.";
RL J. Biol. Chem. 276:19363-19374(2001).
RN [3]
RP PROTEIN SEQUENCE OF 30-57 AND 87-99, AND SUBCELLULAR LOCATION.
RX PubMed=22015679; DOI=10.1016/j.bbapap.2011.09.013;
RA Haque M.E., Koc H., Cimen H., Koc E.C., Spremulli L.L.;
RT "Contacts between mammalian mitochondrial translational initiation factor 3
RT and ribosomal proteins in the small subunit.";
RL Biochim. Biophys. Acta 1814:1779-1784(2011).
CC -!- FUNCTION: May be necessary to recruit DLD/E3 to the mitochondrial 2-
CC oxoglutarate dehydrogenase complex (OGDC) core composed of OGDH/E1-
CC DLST/E2, hence stabilizes the complex. {ECO:0000250|UniProtKB:Q9CQX8}.
CC -!- SUBUNIT: Probable component of the 2-oxoglutarate dehydrogenase complex
CC (OGDC), composed of OGDH (2-oxoglutarate dehydrogenase; also called E1
CC subunit), DLST (dihydrolipoamide succinyltransferase; also called E2
CC subunit) and DLD (dihydrolipoamide dehydrogenase; also called E3
CC subunit). Within OGDC, may interact (via N-terminus) with E3 subunit
CC and (via C-terminus) with the complex core formed by E1 and E2
CC subunits. {ECO:0000250|UniProtKB:Q9CQX8}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:11279123,
CC ECO:0000269|PubMed:22015679}.
CC -!- CAUTION: Was originally identified in the small subunit (28S) of
CC mitochondrial ribosomes that were purified on sucrose gradients
CC (PubMed:11279123, PubMed:22015679). This observation has been
CC challenged by experiments showing MRPS36 copurification with the
CC oxoglutarate dehydrogenase complex (OGDC), also called alpha-
CC ketoglutarate dehydrogenase complex (KGDH). Both mitochondrial ribosome
CC 28S subunit and OGDC have a similar size and OGDC is highly abundant,
CC therefore OGDC has been found to contaminate ribosomal preparations
CC performed by sequential centrifugation steps (By similarity). In
CC addition, MRPS36 could not be located in the structure of the human
CC mitochondrial ribosome, supporting the hypothesis that it is not a
CC mitoribosomal protein (By similarity). {ECO:0000250|UniProtKB:P82909,
CC ECO:0000250|UniProtKB:Q9CQX8, ECO:0000269|PubMed:11279123,
CC ECO:0000269|PubMed:22015679}.
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DR EMBL; BC103454; AAI03455.1; -; mRNA.
DR RefSeq; NP_001030515.1; NM_001035438.1.
DR AlphaFoldDB; P82908; -.
DR SMR; P82908; -.
DR CORUM; P82908; -.
DR IntAct; P82908; 1.
DR STRING; 9913.ENSBTAP00000014665; -.
DR PaxDb; P82908; -.
DR PeptideAtlas; P82908; -.
DR PRIDE; P82908; -.
DR Ensembl; ENSBTAT00000014665; ENSBTAP00000014665; ENSBTAG00000011045.
DR GeneID; 613835; -.
DR KEGG; bta:613835; -.
DR CTD; 92259; -.
DR VEuPathDB; HostDB:ENSBTAG00000011045; -.
DR VGNC; VGNC:31674; MRPS36.
DR eggNOG; ENOG502S7A7; Eukaryota.
DR GeneTree; ENSGT00390000017443; -.
DR HOGENOM; CLU_135102_0_0_1; -.
DR InParanoid; P82908; -.
DR OMA; DRKGYPK; -.
DR OrthoDB; 1643642at2759; -.
DR TreeFam; TF333436; -.
DR Proteomes; UP000009136; Chromosome 20.
DR Bgee; ENSBTAG00000011045; Expressed in oviduct epithelium and 104 other tissues.
DR GO; GO:0005743; C:mitochondrial inner membrane; TAS:Reactome.
DR GO; GO:0009353; C:mitochondrial oxoglutarate dehydrogenase complex; ISO:UniProtKB.
DR GO; GO:0006103; P:2-oxoglutarate metabolic process; ISO:UniProtKB.
DR GO; GO:0006099; P:tricarboxylic acid cycle; ISO:UniProtKB.
DR InterPro; IPR020373; Kgd4/YMR-31.
DR PANTHER; PTHR31601; PTHR31601; 1.
PE 1: Evidence at protein level;
KW Acetylation; Direct protein sequencing; Mitochondrion; Phosphoprotein;
KW Reference proteome; Tricarboxylic acid cycle.
FT CHAIN 1..103
FT /note="Alpha-ketoglutarate dehydrogenase component 4"
FT /id="PRO_0000087732"
FT REGION 23..70
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 42..61
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:P82909"
FT MOD_RES 5
FT /note="N6-succinyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q9CQX8"
FT MOD_RES 61
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P82909"
FT MOD_RES 90
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P82909"
SQ SEQUENCE 103 AA; 11543 MW; B79BE89385D89E2C CRC64;
MMGSKMASAS RVVQVVKPHT PLIRFPDRRD NPKPNVSEVL RSAGLPSHTS SISQHSKGSK
SPDWLMHQGP PDTAEMIKTL PQKYRRKLVS QEEIEFIQRG GPE
