ID   53DR_STAES              Reviewed;         179 AA.
AC   Q8CTG7;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Putative 5'(3')-deoxyribonucleotidase;
DE            EC=3.1.3.-;
GN   OrderedLocusNames=SE_0505;
OS   Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=176280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 12228 / FDA PCI 1200;
RX   PubMed=12950922; DOI=10.1046/j.1365-2958.2003.03671.x;
RA   Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q.,
RA   Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H.,
RA   Zhao G.-P., Qu D., Danchin A., Wen Y.-M.;
RT   "Genome-based analysis of virulence genes in a non-biofilm-forming
RT   Staphylococcus epidermidis strain (ATCC 12228).";
RL   Mol. Microbiol. 49:1577-1593(2003).
CC   -!- FUNCTION: Dephosphorylates the 5' and 2'(3')-phosphates of
CC       deoxyribonucleotides. {ECO:0000305}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:Q97JQ5};
CC   -!- SIMILARITY: Belongs to the 5'(3')-deoxyribonucleotidase family.
CC       {ECO:0000305}.
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DR   EMBL; AE015929; AAO04102.1; -; Genomic_DNA.
DR   RefSeq; NP_764060.1; NC_004461.1.
DR   RefSeq; WP_002468869.1; NZ_WBME01000015.1.
DR   PDB; 3BWV; X-ray; 1.55 A; A/B=1-179.
DR   PDBsum; 3BWV; -.
DR   AlphaFoldDB; Q8CTG7; -.
DR   SMR; Q8CTG7; -.
DR   DNASU; 1056034; -.
DR   EnsemblBacteria; AAO04102; AAO04102; SE_0505.
DR   KEGG; sep:SE_0505; -.
DR   PATRIC; fig|176280.10.peg.477; -.
DR   eggNOG; COG4502; Bacteria.
DR   HOGENOM; CLU_111510_0_0_9; -.
DR   OMA; FNAKFRW; -.
DR   EvolutionaryTrace; Q8CTG7; -.
DR   Proteomes; UP000001411; Chromosome.
DR   GO; GO:0008253; F:5'-nucleotidase activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009264; P:deoxyribonucleotide catabolic process; IEA:InterPro.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR010708; 5'(3')-deoxyribonucleotidase.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR023214; HAD_sf.
DR   Pfam; PF06941; NT5C; 1.
DR   SUPFAM; SSF56784; SSF56784; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Hydrolase; Magnesium; Metal-binding.
FT   CHAIN           1..179
FT                   /note="Putative 5'(3')-deoxyribonucleotidase"
FT                   /id="PRO_0000164386"
FT   ACT_SITE        9
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000305"
FT   ACT_SITE        11
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000305"
FT   BINDING         9
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0007744|PDB:3BWV"
FT   BINDING         11
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0007744|PDB:3BWV"
FT   BINDING         135
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0007744|PDB:3BWV"
FT   STRAND          5..9
FT                   /evidence="ECO:0007829|PDB:3BWV"
FT   TURN            12..14
FT                   /evidence="ECO:0007829|PDB:3BWV"
FT   HELIX           17..28
FT                   /evidence="ECO:0007829|PDB:3BWV"
FT   HELIX           35..37
FT                   /evidence="ECO:0007829|PDB:3BWV"
FT   HELIX           53..59
FT                   /evidence="ECO:0007829|PDB:3BWV"
FT   HELIX           63..65
FT                   /evidence="ECO:0007829|PDB:3BWV"
FT   HELIX           73..80
FT                   /evidence="ECO:0007829|PDB:3BWV"
FT   TURN            81..83
FT                   /evidence="ECO:0007829|PDB:3BWV"
FT   STRAND          84..90
FT                   /evidence="ECO:0007829|PDB:3BWV"
FT   HELIX           98..109
FT                   /evidence="ECO:0007829|PDB:3BWV"
FT   HELIX           115..117
FT                   /evidence="ECO:0007829|PDB:3BWV"
FT   STRAND          118..120
FT                   /evidence="ECO:0007829|PDB:3BWV"
FT   HELIX           124..126
FT                   /evidence="ECO:0007829|PDB:3BWV"
FT   STRAND          130..135
FT                   /evidence="ECO:0007829|PDB:3BWV"
FT   HELIX           137..142
FT                   /evidence="ECO:0007829|PDB:3BWV"
FT   STRAND          144..150
FT                   /evidence="ECO:0007829|PDB:3BWV"
FT   HELIX           153..155
FT                   /evidence="ECO:0007829|PDB:3BWV"
FT   STRAND          160..164
FT                   /evidence="ECO:0007829|PDB:3BWV"
FT   HELIX           167..177
FT                   /evidence="ECO:0007829|PDB:3BWV"
SQ   SEQUENCE   179 AA;  20993 MW;  9A5BA6437DE45A6A CRC64;
     MTRQRIAIDM DEVLADTLGA VVKAVNERAD LNIKMESLNG KKLKHMIPEH EGLVMDILKE
     PGFFRNLDVM PHAQEVVKQL NEHYDIYIAT AAMDVPTSFH DKYEWLLEYF PFLDPQHFVF
     CGRKNIILAD YLIDDNPKQL EIFEGKSIMF TASHNVYEHR FERVSGWRDV KNYFNSIEK