ID   ARAA_OCEIH              Reviewed;         474 AA.
AC   Q8EMP4;
DT   06-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 2.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=L-arabinose isomerase {ECO:0000255|HAMAP-Rule:MF_00519};
DE            EC=5.3.1.4 {ECO:0000255|HAMAP-Rule:MF_00519};
GN   Name=araA {ECO:0000255|HAMAP-Rule:MF_00519}; OrderedLocusNames=OB2797;
OS   Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC
OS   3954 / HTE831).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Oceanobacillus.
OX   NCBI_TaxID=221109;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831;
RX   PubMed=12235376; DOI=10.1093/nar/gkf526;
RA   Takami H., Takaki Y., Uchiyama I.;
RT   "Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge
RT   and its unexpected adaptive capabilities to extreme environments.";
RL   Nucleic Acids Res. 30:3927-3935(2002).
CC   -!- FUNCTION: Catalyzes the conversion of L-arabinose to L-ribulose.
CC       {ECO:0000255|HAMAP-Rule:MF_00519}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=beta-L-arabinopyranose = L-ribulose; Xref=Rhea:RHEA:14821,
CC         ChEBI:CHEBI:16880, ChEBI:CHEBI:40886; EC=5.3.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00519};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00519};
CC       Note=Binds 1 Mn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_00519};
CC   -!- PATHWAY: Carbohydrate degradation; L-arabinose degradation via L-
CC       ribulose; D-xylulose 5-phosphate from L-arabinose (bacterial route):
CC       step 1/3. {ECO:0000255|HAMAP-Rule:MF_00519}.
CC   -!- SIMILARITY: Belongs to the arabinose isomerase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00519}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAC14753.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; BA000028; BAC14753.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_041544585.1; NC_004193.1.
DR   AlphaFoldDB; Q8EMP4; -.
DR   SMR; Q8EMP4; -.
DR   STRING; 221109.22778484; -.
DR   EnsemblBacteria; BAC14753; BAC14753; BAC14753.
DR   KEGG; oih:OB2797; -.
DR   eggNOG; COG2160; Bacteria.
DR   HOGENOM; CLU_045663_0_0_9; -.
DR   OrthoDB; 507566at2; -.
DR   PhylomeDB; Q8EMP4; -.
DR   BRENDA; 5.3.1.4; 4380.
DR   UniPathway; UPA00145; UER00565.
DR   Proteomes; UP000000822; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0008733; F:L-arabinose isomerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0019569; P:L-arabinose catabolic process to xylulose 5-phosphate; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10940; -; 1.
DR   HAMAP; MF_00519; Arabinose_Isome; 1.
DR   InterPro; IPR024664; Ara_Isoase_C.
DR   InterPro; IPR038583; AraA_N_sf.
DR   InterPro; IPR004216; Fuc/Ara_isomerase_C.
DR   InterPro; IPR009015; Fucose_isomerase_N/cen_sf.
DR   InterPro; IPR003762; Lara_isomerase.
DR   PANTHER; PTHR38464; PTHR38464; 1.
DR   Pfam; PF11762; Arabinose_Iso_C; 1.
DR   Pfam; PF02610; Arabinose_Isome; 1.
DR   PIRSF; PIRSF001478; L-ara_isomerase; 1.
DR   SUPFAM; SSF50443; SSF50443; 1.
DR   SUPFAM; SSF53743; SSF53743; 1.
PE   3: Inferred from homology;
KW   Arabinose catabolism; Carbohydrate metabolism; Isomerase; Manganese;
KW   Metal-binding; Reference proteome.
FT   CHAIN           1..474
FT                   /note="L-arabinose isomerase"
FT                   /id="PRO_0000198391"
FT   BINDING         306
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00519"
FT   BINDING         331
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00519"
FT   BINDING         348
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00519"
FT   BINDING         447
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00519"
SQ   SEQUENCE   474 AA;  53283 MW;  86B0C87FF8F967B0 CRC64;
     MLTLKEKEFW FVVGSQQLYG EEALQEVKAQ AQTVTDALNE SGALPYPIVL KDLAINADQI
     TNVMKEVNYR DEVAGVITWM HTFSPAKMWI RGTKLLQKPL LHLATQFNES IPWDTIDMDF
     MNLNQSAHGD REYGFINARL NKQNKVVVGY WGKSEVQRQI AEWMDVAAAY NESFNVKVAR
     FGDNMRNVAV TDGDKIEAQI QFGWTVDYFA IGDLVQYVNA VKEEEIDVLF AEYKDSYDFD
     YGDYSKDDWE ASVRVQASYE IAIKRFLDDG GYNAFTSNFE DLYGMKQLPG LAVQRLMGQG
     YGFAGEGDWK TAALDRLLKV MSHNQSTGFM EDYTYELAEG KEAILQSHML EVDPTLASTK
     PKITVFPLGI GDREDPARLV FDGKAGDGVV VSMADFGTHF KLLINEVNAF EPTTPAPNLP
     VARVLWEVKP NFQDGVKAWI ENGGGHHTVV SLNLTTDQVV SYAKLVGLDY IVIK