ID   KGX4C_CENSC             Reviewed;          43 AA.
AC   P59940;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2003, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Potassium channel toxin gamma-KTx 4.12 {ECO:0000303|PubMed:12459475};
DE   AltName: Full=CsEKerg1 {ECO:0000303|PubMed:12076661};
OS   Centruroides sculpturatus (Arizona bark scorpion).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Centruroides.
OX   NCBI_TaxID=218467;
RN   [1]
RP   PROTEIN SEQUENCE, MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND FUNCTION.
RC   TISSUE=Venom;
RX   PubMed=12076661; DOI=10.1016/s0041-0101(02)00100-9;
RA   Nastainczyk W., Meves H., Watt D.D.;
RT   "A short-chain peptide toxin isolated from Centruroides sculpturatus
RT   scorpion venom inhibits ether-a-go-go-related gene K(+) channels.";
RL   Toxicon 40:1053-1058(2002).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=12459475; DOI=10.1016/s0014-5793(02)03652-9;
RA   Corona M., Gurrola G.B., Merino E., Cassulini R.R., Valdez-Cruz N.A.,
RA   Garcia B., Ramirez-Dominguez M.E., Coronas F.I., Zamudio F.Z., Wanke E.,
RA   Possani L.D.;
RT   "A large number of novel Ergtoxin-like genes and ERG K+-channels blocking
RT   peptides from scorpions of the genus Centruroides.";
RL   FEBS Lett. 532:121-126(2002).
CC   -!- FUNCTION: Reversibly blocks Kv11/ERG potassium channels. Is less toxic
CC       than ergtoxin (AC Q86QT3). {ECO:0000269|PubMed:12076661}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:12076661}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- DOMAIN: Has the CSalpha/beta fold, which comprises one or two short
CC       alpha helices connected to anti-parallel beta-sheets stabilized by
CC       three or four disulfide bonds. {ECO:0000250|UniProtKB:Q86QT3}.
CC   -!- MASS SPECTROMETRY: Mass=4832.1; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:12076661};
CC   -!- SIMILARITY: Belongs to the ergtoxin family. Gamma-KTx 4 subfamily.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P59940; -.
DR   SMR; P59940; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019870; F:potassium channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR012622; Ergtoxin.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   Pfam; PF08086; Toxin_17; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS60026; ERGTX; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Knottin; Neurotoxin; Potassium channel impairing toxin; Secreted; Toxin;
KW   Voltage-gated potassium channel impairing toxin.
FT   CHAIN           1..43
FT                   /note="Potassium channel toxin gamma-KTx 4.12"
FT                   /id="PRO_0000066859"
FT   DISULFID        5..23
FT                   /evidence="ECO:0000250|UniProtKB:Q86QT3"
FT   DISULFID        11..34
FT                   /evidence="ECO:0000250|UniProtKB:Q86QT3"
FT   DISULFID        20..39
FT                   /evidence="ECO:0000250|UniProtKB:Q86QT3"
FT   DISULFID        24..41
FT                   /evidence="ECO:0000250|UniProtKB:Q86QT3"
SQ   SEQUENCE   43 AA;  4842 MW;  BDA4C3B66772EA62 CRC64;
     ERDSCVEKSK CGKYGYYGQC DECCKKAGDR AGTCVYYKCK CNP