ID   KHDC4_DANRE             Reviewed;         570 AA.
AC   Q6NZ18; Q7ZVX3;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=KH homology domain-containing protein 4;
DE   AltName: Full=Brings lots of money 7 {ECO:0000250|UniProtKB:Q7Z7F0};
DE   AltName: Full=Pre-mRNA splicing factor protein khdc4;
GN   Name=khdc4; Synonyms=blom7 {ECO:0000250|UniProtKB:Q7Z7F0}, Kiaa0907;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA-binding protein involved in pre-mRNA splicing. Interacts
CC       with the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the
CC       spliceosome. Involved in regulating splice site selection. Binds
CC       preferentially RNA with A/C rich sequences and poly-C stretches.
CC       {ECO:0000250|UniProtKB:Q7Z7F0}.
CC   -!- SUBUNIT: Interacts with PRPF19. {ECO:0000250|UniProtKB:Q7Z7F0}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q7Z7F0}. Cytoplasm
CC       {ECO:0000250|UniProtKB:Q7Z7F0}.
CC   -!- DOMAIN: The C-terminal part is necessary for the interaction with the
CC       PRP19C/Prp19 complex/NTC/Nineteen complex.
CC       {ECO:0000250|UniProtKB:Q7Z7F0}.
CC   -!- DOMAIN: The KH domains mediate RNA-binding.
CC       {ECO:0000250|UniProtKB:Q7Z7F0}.
CC   -!- SIMILARITY: Belongs to the KHDC4 family. {ECO:0000305}.
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DR   EMBL; BC045375; AAH45375.1; -; mRNA.
DR   EMBL; BC066381; AAH66381.1; -; mRNA.
DR   RefSeq; NP_997758.1; NM_212593.1.
DR   AlphaFoldDB; Q6NZ18; -.
DR   SMR; Q6NZ18; -.
DR   STRING; 7955.ENSDARP00000059559; -.
DR   PaxDb; Q6NZ18; -.
DR   GeneID; 321686; -.
DR   KEGG; dre:321686; -.
DR   CTD; 22889; -.
DR   ZFIN; ZDB-GENE-030131-405; khdc4.
DR   eggNOG; KOG1960; Eukaryota.
DR   InParanoid; Q6NZ18; -.
DR   OrthoDB; 633868at2759; -.
DR   PhylomeDB; Q6NZ18; -.
DR   PRO; PR:Q6NZ18; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR   GO; GO:0006376; P:mRNA splice site selection; ISS:UniProtKB.
DR   Gene3D; 3.30.1370.10; -; 2.
DR   InterPro; IPR036612; KH_dom_type_1_sf.
DR   InterPro; IPR031121; RIK/BLOM7.
DR   PANTHER; PTHR15744; PTHR15744; 1.
DR   SUPFAM; SSF54791; SSF54791; 2.
PE   2: Evidence at transcript level;
KW   Cytoplasm; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW   Reference proteome; RNA-binding.
FT   CHAIN           1..570
FT                   /note="KH homology domain-containing protein 4"
FT                   /id="PRO_0000296672"
FT   DOMAIN          77..157
FT                   /note="KH 1"
FT                   /evidence="ECO:0000250|UniProtKB:Q7Z7F0"
FT   DOMAIN          210..292
FT                   /note="KH 2"
FT                   /evidence="ECO:0000250|UniProtKB:Q7Z7F0"
FT   REGION          1..29
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          391..410
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          427..497
FT                   /note="Required for nuclear retention"
FT                   /evidence="ECO:0000250|UniProtKB:Q7Z7F0"
FT   REGION          437..500
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          512..570
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        391..405
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        440..462
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        540..554
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        555..570
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        464
FT                   /note="Missing (in Ref. 1; AAH45375)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   570 AA;  60376 MW;  D58A37176562AC17 CRC64;
     MSFGVPHSGG SRRSKWDQAG PDADAGSAPT GALDAAAAVA AKINAMLVAK GKLKPSQISS
     AAPVDKAAGA GGNKLKDDLV VAEVEINDVP LTCRNLLTRG QTQDEISRVS GAAVSTRGRF
     MTVEEKSKAL PSDRPLYLHV QGQTRDLVDK AVNRIKEIIT NGVVKAATNS TYSGATVTVY
     QQSGPSVPTI PSAPHKPHYP GGMHYVQDKV FVGLDQALQG FNVKERVEGP SCSFLQHIQA
     ETGAKVFLRG KGSGCLEPAS GREAFEPMYI YISHPKPEGL ASAKTLCENL LQTVHAEYSR
     YLNQMSSMMP TQGFIHPPVV NGLPPQPPYY SPAGFQPSYP APVPPPPPPI APQYPVAPVA
     PAPVPPPNAQ YPITPVPAHV PTQTLLPAAF PPTAPVPPKL TAPPNPPQKR RFTEEVDEMD
     RGLLGYQHGP IHMTNLGAGM PVGSSETSGP PSAASSVPVR ERDSSRQLMP PPCAPALLKP
     LRPLKADESP SAPSLLEPQV KRMRTGLVAY AGDSSDEEED HGPSRAAVTA AGNPGAGWNP
     YRCPPSPPHR PKTQTAPQPT QQNMPFWMAP