KHNYN_HUMAN
ID KHNYN_HUMAN Reviewed; 678 AA.
AC O15037; Q86TZ6; Q8IUQ2; Q96BA9;
DT 29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2011, sequence version 3.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Protein KHNYN;
DE AltName: Full=KH and NYN domain-containing protein;
GN Name=KHNYN; Synonyms=KIAA0323;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS THR-261 AND LEU-270.
RC TISSUE=Brain;
RX PubMed=9205841; DOI=10.1093/dnares/4.2.141;
RA Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Miyajima N.,
RA Tanaka A., Kotani H., Nomura N., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. VII. The
RT complete sequences of 100 new cDNA clones from brain which can code for
RT large proteins in vitro.";
RL DNA Res. 4:141-150(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Placenta;
RA Li W.B., Gruber C., Jessee J., Polayes D.;
RT "Full-length cDNA libraries and normalization.";
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12508121; DOI=10.1038/nature01348;
RA Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C.,
RA Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A.,
RA Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H.,
RA Du H., Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T.,
RA Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B.,
RA Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D.,
RA Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R.,
RA Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S.,
RA Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C.,
RA Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S.,
RA Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C.,
RA Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P.,
RA Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M.,
RA Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V.,
RA Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J.,
RA Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F.,
RA Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F.,
RA Waterston R., Hood L., Weissenbach J.;
RT "The DNA sequence and analysis of human chromosome 14.";
RL Nature 421:601-607(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT LEU-270.
RC TISSUE=Brain, and Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-359, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- INTERACTION:
CC O15037; Q15843: NEDD8; NbExp=13; IntAct=EBI-6148525, EBI-716247;
CC O15037; O60568: PLOD3; NbExp=3; IntAct=EBI-6148525, EBI-741582;
CC O15037; Q9NWF9: RNF216; NbExp=3; IntAct=EBI-6148525, EBI-723313;
CC O15037; Q96N21: TEPSIN; NbExp=3; IntAct=EBI-6148525, EBI-11139477;
CC -!- SIMILARITY: Belongs to the N4BP1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA20781.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB002321; BAA20781.1; ALT_INIT; mRNA.
DR EMBL; BX161465; CAD61924.1; -; mRNA.
DR EMBL; AL132800; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC015780; AAH15780.1; -; mRNA.
DR EMBL; BC042554; AAH42554.1; -; mRNA.
DR CCDS; CCDS32058.1; -.
DR RefSeq; NP_001277185.1; NM_001290256.1.
DR RefSeq; NP_001277186.1; NM_001290257.1.
DR RefSeq; NP_056114.1; NM_015299.2.
DR RefSeq; XP_005267531.1; XM_005267474.4.
DR PDB; 2N5M; NMR; -; A=627-678.
DR PDB; 2N7K; NMR; -; B=627-678.
DR PDBsum; 2N5M; -.
DR PDBsum; 2N7K; -.
DR AlphaFoldDB; O15037; -.
DR SMR; O15037; -.
DR BioGRID; 116933; 16.
DR IntAct; O15037; 13.
DR MINT; O15037; -.
DR STRING; 9606.ENSP00000251343; -.
DR iPTMnet; O15037; -.
DR MetOSite; O15037; -.
DR PhosphoSitePlus; O15037; -.
DR BioMuta; KHNYN; -.
DR EPD; O15037; -.
DR jPOST; O15037; -.
DR MassIVE; O15037; -.
DR MaxQB; O15037; -.
DR PaxDb; O15037; -.
DR PeptideAtlas; O15037; -.
DR PRIDE; O15037; -.
DR ProteomicsDB; 48394; -.
DR Antibodypedia; 186; 78 antibodies from 16 providers.
DR DNASU; 23351; -.
DR Ensembl; ENST00000251343.9; ENSP00000251343.5; ENSG00000100441.10.
DR Ensembl; ENST00000553935.6; ENSP00000450799.1; ENSG00000100441.10.
DR Ensembl; ENST00000556842.5; ENSP00000451106.1; ENSG00000100441.10.
DR GeneID; 23351; -.
DR KEGG; hsa:23351; -.
DR MANE-Select; ENST00000553935.6; ENSP00000450799.1; NM_015299.3; NP_056114.1.
DR UCSC; uc001wph.5; human.
DR CTD; 23351; -.
DR DisGeNET; 23351; -.
DR GeneCards; KHNYN; -.
DR HGNC; HGNC:20166; KHNYN.
DR HPA; ENSG00000100441; Low tissue specificity.
DR MIM; 619579; gene.
DR neXtProt; NX_O15037; -.
DR OpenTargets; ENSG00000100441; -.
DR PharmGKB; PA165479143; -.
DR VEuPathDB; HostDB:ENSG00000100441; -.
DR eggNOG; KOG3777; Eukaryota.
DR GeneTree; ENSGT00940000161993; -.
DR HOGENOM; CLU_014137_0_0_1; -.
DR InParanoid; O15037; -.
DR OMA; NQQQGRE; -.
DR OrthoDB; 771251at2759; -.
DR PhylomeDB; O15037; -.
DR TreeFam; TF315783; -.
DR PathwayCommons; O15037; -.
DR SignaLink; O15037; -.
DR BioGRID-ORCS; 23351; 13 hits in 1077 CRISPR screens.
DR ChiTaRS; KHNYN; human.
DR GenomeRNAi; 23351; -.
DR Pharos; O15037; Tdark.
DR PRO; PR:O15037; -.
DR Proteomes; UP000005640; Chromosome 14.
DR RNAct; O15037; protein.
DR Bgee; ENSG00000100441; Expressed in left ovary and 180 other tissues.
DR ExpressionAtlas; O15037; baseline and differential.
DR Genevisible; O15037; HS.
DR GO; GO:0036464; C:cytoplasmic ribonucleoprotein granule; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004521; F:endoribonuclease activity; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0090502; P:RNA phosphodiester bond hydrolysis, endonucleolytic; IBA:GO_Central.
DR InterPro; IPR036612; KH_dom_type_1_sf.
DR InterPro; IPR021869; RNase_Zc3h12_NYN.
DR Pfam; PF11977; RNase_Zc3h12a; 1.
DR SUPFAM; SSF54791; SSF54791; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Phosphoprotein; Reference proteome.
FT CHAIN 1..678
FT /note="Protein KHNYN"
FT /id="PRO_0000084290"
FT DOMAIN 437..589
FT /note="RNase NYN"
FT /evidence="ECO:0000255"
FT REGION 222..251
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 347..407
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 595..633
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 354..368
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 371..391
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 614..633
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 10
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 359
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT VARIANT 261
FT /note="K -> T (in dbSNP:rs3742520)"
FT /evidence="ECO:0000269|PubMed:9205841"
FT /id="VAR_030843"
FT VARIANT 270
FT /note="W -> L (in dbSNP:rs7151995)"
FT /evidence="ECO:0000269|PubMed:15489334,
FT ECO:0000269|PubMed:9205841"
FT /id="VAR_030844"
FT HELIX 632..640
FT /evidence="ECO:0007829|PDB:2N5M"
FT TURN 646..651
FT /evidence="ECO:0007829|PDB:2N5M"
FT HELIX 652..658
FT /evidence="ECO:0007829|PDB:2N5M"
FT HELIX 662..677
FT /evidence="ECO:0007829|PDB:2N5M"
SQ SEQUENCE 678 AA; 74534 MW; 4E52D096B545430D CRC64;
MPTWGARPAS PDRFAVSAEA ENKVREQQPH VERIFSVGVS VLPKDCPDNP HIWLQLEGPK
ENASRAKEYL KGLCSPELQD EIHYPPKLHC IFLGAQGFFL DCLAWSTSAH LVPRAPGSLM
ISGLTEAFVM AQSRVEELAE RLSWDFTPGP SSGASQCTGV LRDFSALLQS PGDAHREALL
QLPLAVQEEL LSLVQEASSG QGPGALASWE GRSSALLGAQ CQGVRAPPSD GRESLDTGSM
GPGDCRGARG DTYAVEKEGG KQGGPREMDW GWKELPGEEA WEREVALRPQ SVGGGARESA
PLKGKALGKE EIALGGGGFC VHREPPGAHG SCHRAAQSRG ASLLQRLHNG NASPPRVPSP
PPAPEPPWHC GDRGDCGDRG DVGDRGDKQQ GMARGRGPQW KRGARGGNLV TGTQRFKEAL
QDPFTLCLAN VPGQPDLRHI VIDGSNVAMV HGLQHYFSSR GIAIAVQYFW DRGHRDITVF
VPQWRFSKDA KVRESHFLQK LYSLSLLSLT PSRVMDGKRI SSYDDRFMVK LAEETDGIIV
SNDQFRDLAE ESEKWMAIIR ERLLPFTFVG NLFMVPDDPL GRNGPTLDEF LKKPARTQGS
SKAQHPSRGF AEHGKQQQGR EEEKGSGGIR KTRETERLRR QLLEVFWGQD HKVDFILQRE
PYCRDINQLS EALLSLNF
