KHPB_LACPL
ID KHPB_LACPL Reviewed; 322 AA.
AC F9ULM5;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 19-OCT-2011, sequence version 1.
DT 03-AUG-2022, entry version 55.
DE RecName: Full=RNA-binding protein KhpB {ECO:0000255|HAMAP-Rule:MF_00867};
DE AltName: Full=RNA-binding protein EloR {ECO:0000255|HAMAP-Rule:MF_00867, ECO:0000303|PubMed:30894429};
DE AltName: Full=Single stranded DNA-binding protein {ECO:0000312|EMBL:CCC80632.1};
GN Name=khpB {ECO:0000255|HAMAP-Rule:MF_00867};
GN Synonyms=eloR {ECO:0000255|HAMAP-Rule:MF_00867,
GN ECO:0000303|PubMed:30894429}; OrderedLocusNames=lp_3683;
OS Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
OS (Lactobacillus plantarum).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactiplantibacillus.
OX NCBI_TaxID=220668;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX PubMed=12566566; DOI=10.1073/pnas.0337704100;
RA Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P.,
RA Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J.,
RA Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M.,
RA Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M.,
RA Siezen R.J.;
RT "Complete genome sequence of Lactobacillus plantarum WCFS1.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX PubMed=22156394; DOI=10.1128/jb.06275-11;
RA Siezen R.J., Francke C., Renckens B., Boekhorst J., Wels M.,
RA Kleerebezem M., van Hijum S.A.;
RT "Complete resequencing and reannotation of the Lactobacillus plantarum
RT WCFS1 genome.";
RL J. Bacteriol. 194:195-196(2012).
RN [3]
RP FUNCTION, PROBABLE SUBUNIT, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX PubMed=30894429; DOI=10.1128/msphere.00007-19;
RA Myrbraaten I.S., Wiull K., Salehian Z., Haavarstein L.S., Straume D.,
RA Mathiesen G., Kjos M.;
RT "CRISPR Interference for Rapid Knockdown of Essential Cell Cycle Genes in
RT Lactobacillus plantarum.";
RL MSphere 4:0-0(2019).
CC -!- FUNCTION: A probable RNA chaperone. Forms a complex with KhpA which
CC binds to cellular RNA and controls its expression. Plays a role in
CC peptidoglycan (PG) homeostasis and cell length regulation.
CC {ECO:0000255|HAMAP-Rule:MF_00867}.
CC -!- FUNCTION: Necessary for correct cell elongation.
CC {ECO:0000269|PubMed:30894429}.
CC -!- SUBUNIT: Forms a complex with KhpA. {ECO:0000255|HAMAP-Rule:MF_00867,
CC ECO:0000305|PubMed:30894429}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00867}.
CC -!- DOMAIN: Has an N-terminal Jag-N domain, a linker with a phosphorylated
CC Thr, and 2 RNA-binding domains (KH and R3H). {ECO:0000255|HAMAP-
CC Rule:MF_00867}.
CC -!- DISRUPTION PHENOTYPE: Cell length is reduced.
CC {ECO:0000269|PubMed:30894429}.
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DR EMBL; AL935263; CCC80632.1; -; Genomic_DNA.
DR RefSeq; WP_011102290.1; NC_004567.2.
DR RefSeq; YP_004891146.1; NC_004567.2.
DR STRING; 220668.lp_3683; -.
DR EnsemblBacteria; CCC80632; CCC80632; lp_3683.
DR GeneID; 57026858; -.
DR KEGG; lpl:lp_3683; -.
DR PATRIC; fig|220668.9.peg.3076; -.
DR eggNOG; COG1847; Bacteria.
DR HOGENOM; CLU_042512_0_0_9; -.
DR OMA; INQKHAP; -.
DR PhylomeDB; F9ULM5; -.
DR BioCyc; LPLA220668:G1GW0-3119-MON; -.
DR Proteomes; UP000000432; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR CDD; cd02414; jag_KH; 1.
DR CDD; cd02644; R3H_jag; 1.
DR Gene3D; 3.30.1370.50; -; 1.
DR Gene3D; 3.30.30.80; -; 1.
DR Gene3D; 3.30.300.20; -; 1.
DR HAMAP; MF_00867; KhpB; 1.
DR InterPro; IPR038008; Jag_KH.
DR InterPro; IPR038247; Jag_N_dom_sf.
DR InterPro; IPR015946; KH_dom-like_a/b.
DR InterPro; IPR039247; KhpB.
DR InterPro; IPR032782; KhpB_N.
DR InterPro; IPR001374; R3H_dom.
DR InterPro; IPR036867; R3H_dom_sf.
DR InterPro; IPR034079; R3H_KhpB.
DR PANTHER; PTHR35800; PTHR35800; 1.
DR Pfam; PF14804; Jag_N; 1.
DR Pfam; PF01424; R3H; 1.
DR SMART; SM01245; Jag_N; 1.
DR SMART; SM00393; R3H; 1.
DR SUPFAM; SSF82708; SSF82708; 1.
DR PROSITE; PS51061; R3H; 1.
PE 1: Evidence at protein level;
KW Cell shape; Cell wall biogenesis/degradation; Chaperone; Cytoplasm;
KW Phosphoprotein; Reference proteome; RNA-binding.
FT CHAIN 1..322
FT /note="RNA-binding protein KhpB"
FT /id="PRO_0000454542"
FT DOMAIN 174..251
FT /note="KH"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00867"
FT DOMAIN 256..322
FT /note="R3H"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00867"
FT REGION 3..52
FT /note="Jag_N domain"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00867"
FT REGION 58..162
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 58..114
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 133..162
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 89
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q8CY87"
SQ SEQUENCE 322 AA; 34181 MW; 38EA7C52306580DA CRC64;
MTVFEGNTVA AAIAAGLKQL HRTRDQVEVE VIAEAKKGFL GLGKHPAQVR LTVVPASAAP
ATTPTSATAT AQQSVATEST TAPTMPRPTV QTPKSTPTRQ AKTSQATTSA AKPATSKAKA
VAKPASMAVT TGPVIADTDQ SKPATTSKTK SVAADQSQTP RTPAEIAARQ AANETAVRAL
CDYLLAVVKE LGVTADLDVD FGNRYATLNF DTTKQGLLIG KHGRTINALQ DLAQVYMNHH
GASHVNVVLD VDDYRERRAA TLKRLAESTA REVIATGKQV FLDPMPSFER KLIHAELANN
HHVTTFSEGR DPHRAVVVAI RK
