ID   KHS1_YEASX              Reviewed;         708 AA.
AC   P39690;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Killer toxin KHS;
DE   AltName: Full=Killer of heat sensitive;
DE   Flags: Precursor;
GN   Name=KHS1; Synonyms=KHS;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 37-42.
RC   STRAIN=115;
RX   PubMed=1368726; DOI=10.1271/bbb1961.55.1953;
RA   Goto K., Fukuda H., Kichise K., Kitano K., Hara S.;
RT   "Cloning and nucleotide sequence of the KHS killer gene of Saccharomyces
RT   cerevisiae.";
RL   Agric. Biol. Chem. 55:1953-1958(1991).
CC   -!- FUNCTION: Kills sensitive strains of yeast.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 4-4.5.;
CC       Temperature dependence:
CC         Unstable above 30 degrees Celsius.;
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: To yeast YER187w. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; S77712; AAC60532.1; -; Genomic_DNA.
DR   PIR; JQ1148; JQ1148.
DR   AlphaFoldDB; P39690; -.
DR   PRIDE; P39690; -.
DR   SGD; S000080162; KHS1.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cell membrane; Direct protein sequencing; Membrane; Signal; Toxin;
KW   Transmembrane; Transmembrane helix.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   PROPEP          28..36
FT                   /evidence="ECO:0000269|PubMed:1368726"
FT                   /id="PRO_0000021542"
FT   CHAIN           37..708
FT                   /note="Killer toxin KHS"
FT                   /id="PRO_0000021543"
FT   TRANSMEM        77..97
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        380..400
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        466..486
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   708 AA;  79535 MW;  FB63D9E8C4C27431 CRC64;
     MPRFAIIFAL LIAYSLFLST LFTGSIPDRA NTVTSNAPCQ VVIWDWIRTR RICNCCSRLC
     YSLLGRSNLS RTAKRGVCTI AGAVLATAAV IVAAVLVGKS SGSATKRGLT KTISVLNHTI
     PFTDHILNGQ TLSNGTGSNF VTIGFSGYAV HATIKRASTT DIISWVIPES MEPTLARVAS
     YVSSSSINLA AVPDTGGNAS ALSFQNAVQE FATSWVSMTY DQSYGDLRNV ANDEGGEEIL
     ILMRKRSYRI SFQVIETGST ALLLRTRRVV SQLITMTYLV TVQARVGIQI GDIFQHYGGI
     DNYVMTSISV LRTLEDKAFH ENKLLIVREP PNKSNQDANQ SYRLRPFSAN DLIQNLKSVD
     IGFLAFCSFF DKYAHYPEII MMKITIFISK GNLWSIIYVI QARYVRKRVM KVRGQMPGGL
     LTNMESLLNI VSTPNLNISE FHIQTHSMSQ SKPMYFQKQC YSSQNNIIYI YNSIHITCGA
     VYVIVHDVRT PSVFVLIELR NCKPLKNSWC ETTKTSPRDT KIKKNEYNET VCRRAGALLD
     GRVRTIRFLM MRTHWSRVKG VSCNTANRLS RFCNHVVSYY PSQNATIHLL PTSLRAESLE
     QQYTTRPLSS SNNRFCCLKS IFINNCKKAC ESPSLVSCNL QQTAELLMVY YLYICEACYV
     SRNHDLLSKQ CMSTVRAVYV ARMRLPKFRS TFPCMPRLCW LVNGVVVV