ID   KHSE_AGRFC              Reviewed;         322 AA.
AC   Q8UHA8;
DT   10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Homoserine kinase {ECO:0000255|HAMAP-Rule:MF_00301};
DE            Short=HK {ECO:0000255|HAMAP-Rule:MF_00301};
DE            Short=HSK {ECO:0000255|HAMAP-Rule:MF_00301};
DE            EC=2.7.1.39 {ECO:0000255|HAMAP-Rule:MF_00301};
GN   Name=thrB {ECO:0000255|HAMAP-Rule:MF_00301}; OrderedLocusNames=Atu0775;
GN   ORFNames=AGR_C_1416;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-homoserine = ADP + H(+) + O-phospho-L-homoserine;
CC         Xref=Rhea:RHEA:13985, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57476, ChEBI:CHEBI:57590, ChEBI:CHEBI:456216;
CC         EC=2.7.1.39; Evidence={ECO:0000255|HAMAP-Rule:MF_00301};
CC   -!- PATHWAY: Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine
CC       from L-aspartate: step 4/5. {ECO:0000255|HAMAP-Rule:MF_00301}.
CC   -!- SIMILARITY: Belongs to the pseudomonas-type ThrB family.
CC       {ECO:0000255|HAMAP-Rule:MF_00301}.
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DR   EMBL; AE007869; AAK86584.1; -; Genomic_DNA.
DR   PIR; AI2671; AI2671.
DR   PIR; G97453; G97453.
DR   RefSeq; NP_353799.1; NC_003062.2.
DR   RefSeq; WP_010971141.1; NC_003062.2.
DR   PDB; 2PPQ; X-ray; 2.00 A; A=1-322.
DR   PDBsum; 2PPQ; -.
DR   AlphaFoldDB; Q8UHA8; -.
DR   SMR; Q8UHA8; -.
DR   STRING; 176299.Atu0775; -.
DR   PRIDE; Q8UHA8; -.
DR   EnsemblBacteria; AAK86584; AAK86584; Atu0775.
DR   KEGG; atu:Atu0775; -.
DR   PATRIC; fig|176299.10.peg.775; -.
DR   eggNOG; COG2334; Bacteria.
DR   HOGENOM; CLU_053300_0_0_5; -.
DR   OMA; DPTHFER; -.
DR   PhylomeDB; Q8UHA8; -.
DR   BioCyc; AGRO:ATU0775-MON; -.
DR   UniPathway; UPA00050; UER00064.
DR   EvolutionaryTrace; Q8UHA8; -.
DR   Proteomes; UP000000813; Chromosome circular.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004413; F:homoserine kinase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0009088; P:threonine biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd05153; HomoserineK_II; 1.
DR   HAMAP; MF_00301; Homoser_kinase_2; 1.
DR   InterPro; IPR002575; Aminoglycoside_PTrfase.
DR   InterPro; IPR005280; Homoserine_kinase_II.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   Pfam; PF01636; APH; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
DR   TIGRFAMs; TIGR00938; thrB_alt; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Amino-acid biosynthesis; ATP-binding; Kinase;
KW   Nucleotide-binding; Reference proteome; Threonine biosynthesis;
KW   Transferase.
FT   CHAIN           1..322
FT                   /note="Homoserine kinase"
FT                   /id="PRO_0000172184"
FT   HELIX           9..16
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   STRAND          24..30
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   STRAND          36..45
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   STRAND          48..53
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   HELIX           61..74
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   STRAND          92..94
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   STRAND          97..103
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   HELIX           115..131
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   TURN            132..134
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   HELIX           145..155
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   HELIX           156..161
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   HELIX           166..180
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   STRAND          187..190
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   HELIX           196..198
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   STRAND          199..202
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   STRAND          205..209
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   STRAND          216..219
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   HELIX           220..231
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   HELIX           241..252
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   HELIX           259..286
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   HELIX           299..309
FT                   /evidence="ECO:0007829|PDB:2PPQ"
FT   HELIX           314..317
FT                   /evidence="ECO:0007829|PDB:2PPQ"
SQ   SEQUENCE   322 AA;  36243 MW;  6F1E6CDC53744272 CRC64;
     MAVYTDITED ELRNFLTQYD VGSLTSYKGI AEGVENSNFL LHTTKDPLIL TLYEKRVEKN
     DLPFFLGLMQ HLAAKGLSCP LPLPRKDGEL LGELSGRPAA LISFLEGMWL RKPEAKHCRE
     VGKALAAMHL ASEGFEIKRP NALSVDGWKV LWDKSEERAD EVEKGLREEI RPEIDYLAAH
     WPKDLPAGVI HADLFQDNVF FLGDELSGLI DFYFACNDLL AYDVSICLNA WCFEKDGAYN
     VTKGKALLEG YQSVRPLSEA ELEALPLLSR GSALRFFLTR LYDWLTTPAG ALVVKKDPLE
     YLRKLRFHRT IANVAEYGLA GE