KHSE_LACLC
ID KHSE_LACLC Reviewed; 296 AA.
AC P52991;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Homoserine kinase;
DE Short=HK;
DE Short=HSK;
DE EC=2.7.1.39;
GN Name=thrB;
OS Lactococcus lactis subsp. cremoris (Streptococcus cremoris).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus.
OX NCBI_TaxID=1359;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=MG1614;
RX PubMed=8682767; DOI=10.1128/jb.178.13.3689-3694.1996;
RA Madsen S.M., Albrechtsen B., Hansen E.B., Israelsen H.;
RT "Cloning and transcriptional analysis of two threonine biosynthetic genes
RT from Lactococcus lactis MG1614.";
RL J. Bacteriol. 178:3689-3694(1996).
CC -!- FUNCTION: Catalyzes the ATP-dependent phosphorylation of L-homoserine
CC to L-homoserine phosphate. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-homoserine = ADP + H(+) + O-phospho-L-homoserine;
CC Xref=Rhea:RHEA:13985, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:57476, ChEBI:CHEBI:57590, ChEBI:CHEBI:456216;
CC EC=2.7.1.39;
CC -!- PATHWAY: Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine
CC from L-aspartate: step 4/5.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the GHMP kinase family. Homoserine kinase
CC subfamily. {ECO:0000305}.
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DR EMBL; X96988; CAA65714.1; -; Genomic_DNA.
DR PIR; JC6050; JC6050.
DR RefSeq; WP_011835204.1; NZ_VERW01000002.1.
DR AlphaFoldDB; P52991; -.
DR SMR; P52991; -.
DR OMA; CANRIPH; -.
DR UniPathway; UPA00050; UER00064.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004413; F:homoserine kinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0009088; P:threonine biosynthetic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.30.70.890; -; 1.
DR HAMAP; MF_00384; Homoser_kinase; 1.
DR InterPro; IPR013750; GHMP_kinase_C_dom.
DR InterPro; IPR036554; GHMP_kinase_C_sf.
DR InterPro; IPR006204; GHMP_kinase_N_dom.
DR InterPro; IPR006203; GHMP_knse_ATP-bd_CS.
DR InterPro; IPR000870; Homoserine_kinase.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR Pfam; PF08544; GHMP_kinases_C; 1.
DR Pfam; PF00288; GHMP_kinases_N; 1.
DR PIRSF; PIRSF000676; Homoser_kin; 1.
DR SUPFAM; SSF54211; SSF54211; 1.
DR SUPFAM; SSF55060; SSF55060; 1.
DR TIGRFAMs; TIGR00191; thrB; 1.
DR PROSITE; PS00627; GHMP_KINASES_ATP; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; ATP-binding; Cytoplasm; Kinase;
KW Nucleotide-binding; Threonine biosynthesis; Transferase.
FT CHAIN 1..296
FT /note="Homoserine kinase"
FT /id="PRO_0000156579"
FT BINDING 84..94
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 296 AA; 31893 MW; 1EFA018C4DA89620 CRC64;
MKIIVPATSA NLGAGFDSIG IAVNLYLTVE VLGESRDWKI DHDLGENIPT DERNLLLTTL
SAVLEDKNVA LSAKFHLKMT SEVPLARGLG SSSSVIIAGI ELANQLAKLN LTSDEKLKLA
CEIEGHPDNV APALLGNLVI ASTVAGKTSH IVADFPSCAL LAFVPDYELK TVESRKVLPN
ELTYKEAVAA SSIANVLTAS LLTNNLEVAG QMMEADRFHE SYRASLIPEL QLLREIGHEF
GAYGTYLSGA GPTVMLLVPD DKLTLLTEKI MEKNLTGHLY PLKIDNKGLQ VEESVF