ID   KHSE_METFK              Reviewed;         319 AA.
AC   Q9RAM6; Q1H4F5;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 2.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Homoserine kinase;
DE            Short=HK;
DE            Short=HSK;
DE            EC=2.7.1.39;
GN   Name=thrB; OrderedLocusNames=Mfla_0361;
OS   Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC   Methylophilaceae; Methylobacillus.
OX   NCBI_TaxID=265072;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10589737; DOI=10.1099/00221287-145-11-3273;
RA   Marchenko G.N., Marchenko N.D., Tsygankov Y.D., Chistoserdov A.Y.;
RT   "Organization of threonine biosynthesis genes from the obligate
RT   methylotroph Methylobacillus flagellatus.";
RL   Microbiology 145:3273-3282(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KT / ATCC 51484 / DSM 6875;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Kyrpides N., Anderson I., Richardson P.;
RT   "Complete sequence of Methylobacillus flagellatus KT.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-homoserine = ADP + H(+) + O-phospho-L-homoserine;
CC         Xref=Rhea:RHEA:13985, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57476, ChEBI:CHEBI:57590, ChEBI:CHEBI:456216;
CC         EC=2.7.1.39;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine
CC       from L-aspartate: step 4/5.
CC   -!- SIMILARITY: Belongs to the pseudomonas-type ThrB family. {ECO:0000305}.
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DR   EMBL; L78666; AAF21132.1; -; Genomic_DNA.
DR   EMBL; CP000284; ABE48632.1; -; Genomic_DNA.
DR   RefSeq; WP_011478729.1; NC_007947.1.
DR   AlphaFoldDB; Q9RAM6; -.
DR   SMR; Q9RAM6; -.
DR   STRING; 265072.Mfla_0361; -.
DR   EnsemblBacteria; ABE48632; ABE48632; Mfla_0361.
DR   KEGG; mfa:Mfla_0361; -.
DR   eggNOG; COG2334; Bacteria.
DR   HOGENOM; CLU_053300_0_0_4; -.
DR   OMA; DPTHFER; -.
DR   OrthoDB; 1003984at2; -.
DR   UniPathway; UPA00050; UER00064.
DR   Proteomes; UP000002440; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004413; F:homoserine kinase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0009088; P:threonine biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd05153; HomoserineK_II; 1.
DR   HAMAP; MF_00301; Homoser_kinase_2; 1.
DR   InterPro; IPR002575; Aminoglycoside_PTrfase.
DR   InterPro; IPR005280; Homoserine_kinase_II.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   Pfam; PF01636; APH; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
DR   TIGRFAMs; TIGR00938; thrB_alt; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; ATP-binding; Kinase; Nucleotide-binding;
KW   Reference proteome; Threonine biosynthesis; Transferase.
FT   CHAIN           1..319
FT                   /note="Homoserine kinase"
FT                   /id="PRO_0000172190"
FT   CONFLICT        273..274
FT                   /note="WL -> CV (in Ref. 1; AAF21132)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   319 AA;  35697 MW;  665D6F38E406891B CRC64;
     MSVFTTVSFE QMQQWLKGYD LGELLDLQGI ASGITNTNYF VTTDNGRYVL TLFEEHSAEE
     LPNFLDLMTH LAERGIPCPH PVKNNAGRAL GELNGKPAAL VSCLAGRSLD NPMPQHCAAI
     GEVLARMHIA GASFKAGMSN LRGQEWRIAT AAKVAPFLDE ENHRMLDAQL EFERTFDTRR
     LPRGVIHADL FRDNVLMDGD KVGGVIDFYY ACHDALLYDI AIAVNDWCVN ADCTLDAVRV
     RAFLDAYHAI RPLTGEEHAA WPGMLRVAAM RFWLSRLNDL YFPQAGELTH AKDPAYFERI
     LKHSIAAREQ ILAVWVNAH