ID   KHSE_PSEAE              Reviewed;         316 AA.
AC   P29364; Q9HT78;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   11-JAN-2001, sequence version 2.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Homoserine kinase;
DE            Short=HK;
DE            Short=HSK;
DE            EC=2.7.1.39;
GN   Name=thrB; OrderedLocusNames=PA5495;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=1333566; DOI=10.1111/j.1365-2958.1992.tb01768.x;
RA   Clepet C., Borne F., Krishnapillai V., Baird C., Patte J.-C., Cami B.;
RT   "Isolation, organization and expression of the Pseudomonas aeruginosa
RT   threonine genes.";
RL   Mol. Microbiol. 6:3109-3119(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-homoserine = ADP + H(+) + O-phospho-L-homoserine;
CC         Xref=Rhea:RHEA:13985, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57476, ChEBI:CHEBI:57590, ChEBI:CHEBI:456216;
CC         EC=2.7.1.39;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine
CC       from L-aspartate: step 4/5.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking thrB are still able to grow in a
CC       minimal medium without the addition of threonine.
CC       {ECO:0000269|PubMed:1333566}.
CC   -!- SIMILARITY: Belongs to the pseudomonas-type ThrB family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA46169.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; X65034; CAA46169.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; AE004091; AAG08880.1; -; Genomic_DNA.
DR   PIR; H82958; H82958.
DR   PIR; S27981; S27981.
DR   RefSeq; NP_254182.1; NC_002516.2.
DR   RefSeq; WP_003114124.1; NZ_QZGE01000012.1.
DR   AlphaFoldDB; P29364; -.
DR   SMR; P29364; -.
DR   STRING; 287.DR97_2873; -.
DR   PaxDb; P29364; -.
DR   PRIDE; P29364; -.
DR   EnsemblBacteria; AAG08880; AAG08880; PA5495.
DR   GeneID; 877825; -.
DR   KEGG; pae:PA5495; -.
DR   PATRIC; fig|208964.12.peg.5760; -.
DR   PseudoCAP; PA5495; -.
DR   HOGENOM; CLU_053300_0_0_6; -.
DR   InParanoid; P29364; -.
DR   OMA; DPTHFER; -.
DR   PhylomeDB; P29364; -.
DR   BioCyc; PAER208964:G1FZ6-5622-MON; -.
DR   UniPathway; UPA00050; UER00064.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0019202; F:amino acid kinase activity; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004413; F:homoserine kinase activity; IDA:PseudoCAP.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0009088; P:threonine biosynthetic process; IDA:PseudoCAP.
DR   CDD; cd05153; HomoserineK_II; 1.
DR   HAMAP; MF_00301; Homoser_kinase_2; 1.
DR   InterPro; IPR002575; Aminoglycoside_PTrfase.
DR   InterPro; IPR005280; Homoserine_kinase_II.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   Pfam; PF01636; APH; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
DR   TIGRFAMs; TIGR00938; thrB_alt; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; ATP-binding; Kinase; Nucleotide-binding;
KW   Reference proteome; Threonine biosynthesis; Transferase.
FT   CHAIN           1..316
FT                   /note="Homoserine kinase"
FT                   /id="PRO_0000172194"
SQ   SEQUENCE   316 AA;  35374 MW;  D57307AAEE5B60C9 CRC64;
     MSVFTPLERS TLEAFLAPYD LGRLRDFRGI AEGSENSNFF VSLEHGEFVL TLVERGPVQD
     LPFFIELLDV LHEDGLPVPY ALRTRDGEAL RRLEGKPALL QPRLAGRHER QPNAHHCQEV
     GDLLGHLHAA TRGRILERPS DRGLPWMLEQ GANLAPRLPE QARALLAPAL AEIAALDAER
     PALPRANLHA DLFRDNVLFD GPHLAGLIDF YNACSGWMLY DLAITLNDWC SNTDGSLDPA
     RARALLAAYA NRRPFTALEA EHWPSMLRVA CVRFWLSRLI AAEAFAGQDV LIHDPAEFEI
     RLAQRQNVEI HLPFAL