ID   KHTS_BACSU              Reviewed;         112 AA.
AC   O07534;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=K(+)/H(+) antiporter modulator KhtS {ECO:0000305};
GN   Name=khtS {ECO:0000303|PubMed:24330391}; Synonyms=yhaS;
GN   OrderedLocusNames=BSU09870;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9579061; DOI=10.1099/00221287-144-4-859;
RA   Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H.,
RA   Venema G., Bron S.;
RT   "The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus
RT   subtilis chromosome contains several dysfunctional genes, the glyB marker,
RT   many genes encoding transporter proteins, and the ubiquitous hit gene.";
RL   Microbiology 144:859-875(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION OF THE KHTSTU OPERON, AND
RP   INDUCTION.
RX   PubMed=14987767; DOI=10.1016/s0378-1097(03)00959-5;
RA   Fujisawa M., Wada Y., Ito M.;
RT   "Modulation of the K+ efflux activity of Bacillus subtilis YhaU by YhaT and
RT   the C-terminal region of YhaS.";
RL   FEMS Microbiol. Lett. 231:211-217(2004).
RN   [4]
RP   FUNCTION.
RX   PubMed=24330391; DOI=10.1111/mmi.12489;
RA   Chandrangsu P., Dusi R., Hamilton C.J., Helmann J.D.;
RT   "Methylglyoxal resistance in Bacillus subtilis: contributions of
RT   bacillithiol-dependent and independent pathways.";
RL   Mol. Microbiol. 91:706-715(2014).
CC   -!- FUNCTION: Modulates the activity of the potassium/proton antiporter
CC       KhtU (PubMed:14987767). Involved in protection of the cell from
CC       methylglyoxal, a toxic by-product of glycolysis (PubMed:24330391).
CC       {ECO:0000269|PubMed:14987767, ECO:0000269|PubMed:24330391}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:14987767};
CC       Peripheral membrane protein {ECO:0000305|PubMed:14987767}; Cytoplasmic
CC       side {ECO:0000305|PubMed:14987767}.
CC   -!- INDUCTION: Part of the khtSTU operon. Induced by salt stress at
CC       alkaline pH. {ECO:0000269|PubMed:14987767}.
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DR   EMBL; Y14080; CAA74440.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12827.1; -; Genomic_DNA.
DR   PIR; E69819; E69819.
DR   RefSeq; NP_388868.1; NC_000964.3.
DR   RefSeq; WP_010886463.1; NZ_JNCM01000035.1.
DR   AlphaFoldDB; O07534; -.
DR   STRING; 224308.BSU09870; -.
DR   PaxDb; O07534; -.
DR   PRIDE; O07534; -.
DR   EnsemblBacteria; CAB12827; CAB12827; BSU_09870.
DR   GeneID; 939291; -.
DR   KEGG; bsu:BSU09870; -.
DR   PATRIC; fig|224308.43.peg.1028; -.
DR   OMA; YPQETQS; -.
DR   BioCyc; BSUB:BSU09870-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
PE   2: Evidence at transcript level;
KW   Cell membrane; Membrane; Reference proteome.
FT   CHAIN           1..112
FT                   /note="K(+)/H(+) antiporter modulator KhtS"
FT                   /id="PRO_0000049551"
FT   REGION          42..64
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        42..60
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   112 AA;  12778 MW;  2369634BC7890D0D CRC64;
     MRREERNMDK LLISFLLSLF MVYFPPSDVV LPSQFEASTD SYVPMSSYPQ ETQSAKTPSP
     GSMHPAELIK EYSPLAQSVR QLSVKPLDEP LINRLEKALA VPVKYQSNYL RI