KI20A_HUMAN
ID KI20A_HUMAN Reviewed; 890 AA.
AC O95235; B4DL79; D3DQB6;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 188.
DE RecName: Full=Kinesin-like protein KIF20A;
DE AltName: Full=GG10_2;
DE AltName: Full=Mitotic kinesin-like protein 2;
DE Short=MKlp2;
DE AltName: Full=Rab6-interacting kinesin-like protein;
DE AltName: Full=Rabkinesin-6;
GN Name=KIF20A; Synonyms=MKLP2, RAB6KIFL;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Endothelial cell;
RX PubMed=10233894;
RA Horrevoets A.J.G., Fontijn R.D., van Zonneveld A.J., de Vries C.J.M.,
RA ten Cate J.W., Pannekoek H.;
RT "Vascular endothelial genes that are responsive to tumor necrosis factor-
RT alpha in vitro are expressed in atherosclerotic lesions, including
RT inhibitor of apoptosis protein-1, stannin, and two novel genes.";
RL Blood 93:3418-3431(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=10806357; DOI=10.1016/s0378-1119(00)00135-9;
RA Lai F., Fernald A.A., Zhao N., Le Beau M.M.;
RT "cDNA cloning, expression pattern, genomic structure and chromosomal
RT location of RAB6KIFL, a human kinesin-like gene.";
RL Gene 248:117-125(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Tongue;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15372022; DOI=10.1038/nature02919;
RA Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S.,
RA Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M.,
RA She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.,
RA Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M.,
RA Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M.,
RA Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T.,
RA Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A.,
RA Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R.,
RA Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L.,
RA Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N.,
RA Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J.,
RA Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A.,
RA Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.;
RT "The DNA sequence and comparative analysis of human chromosome 5.";
RL Nature 431:268-274(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP SUBCELLULAR LOCATION, INTERACTION WITH PLK1, PHOSPHORYLATION AT SER-528;
RP SER-662 AND SER-668, MUTAGENESIS OF SER-528, AND FUNCTION.
RX PubMed=12939256; DOI=10.1083/jcb.200306009;
RA Neef R., Preisinger C., Sutcliffe J., Kopajtich R., Nigg E.A., Mayer T.U.,
RA Barr F.A.;
RT "Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is
RT required for cytokinesis.";
RL J. Cell Biol. 162:863-875(2003).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=17081983; DOI=10.1016/j.cell.2006.09.026;
RA Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.;
RT "Global, in vivo, and site-specific phosphorylation dynamics in signaling
RT networks.";
RL Cell 127:635-648(2006).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=16964243; DOI=10.1038/nbt1240;
RA Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.;
RT "A probability-based approach for high-throughput protein phosphorylation
RT analysis and site localization.";
RL Nat. Biotechnol. 24:1285-1292(2006).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18220336; DOI=10.1021/pr0705441;
RA Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III;
RT "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient
RT phosphoproteomic analysis.";
RL J. Proteome Res. 7:1346-1351(2008).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18691976; DOI=10.1016/j.molcel.2008.07.007;
RA Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,
RA Greff Z., Keri G., Stemmann O., Mann M.;
RT "Kinase-selective enrichment enables quantitative phosphoproteomics of the
RT kinome across the cell cycle.";
RL Mol. Cell 31:438-448(2008).
RN [12]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-528; SER-532; THR-857 AND
RP SER-867, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [13]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE
RP ANALYSIS] AT SER-7; SER-14; SER-21; SER-532 AND SER-825, CLEAVAGE OF
RP INITIATOR METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [14]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-532, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT "System-wide temporal characterization of the proteome and phosphoproteome
RT of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [15]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-532; SER-685; SER-878 AND
RP SER-883, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [16]
RP VARIANT RCM6 TRP-182, INVOLVEMENT IN RCM6, CHARACTERIZATION OF VARIANT RCM6
RP TRP-182, AND SUBCELLULAR LOCATION.
RX PubMed=29357359; DOI=10.1371/journal.pgen.1007138;
RA Louw J.J., Nunes Bastos R., Chen X., Verdood C., Corveleyn A., Jia Y.,
RA Breckpot J., Gewillig M., Peeters H., Santoro M.M., Barr F., Devriendt K.;
RT "Compound heterozygous loss-of-function mutations in KIF20A are associated
RT with a novel lethal congenital cardiomyopathy in two siblings.";
RL PLoS Genet. 14:e1007138-e1007138(2018).
CC -!- FUNCTION: Mitotic kinesin required for chromosome passenger complex
CC (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved
CC in recruitment of PLK1 to the central spindle. Interacts with guanosine
CC triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor
CC required for the retrograde RAB6 regulated transport of Golgi membranes
CC and associated vesicles along microtubules. Has a microtubule plus end-
CC directed motility. {ECO:0000269|PubMed:12939256}.
CC -!- INTERACTION:
CC O95235; Q7L5A3: FAM214B; NbExp=4; IntAct=EBI-2551319, EBI-745689;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus {ECO:0000250}. Cytoplasm,
CC cytoskeleton, spindle {ECO:0000269|PubMed:12939256,
CC ECO:0000269|PubMed:29357359}. Note=Localizes to the spindle midzone
CC during anaphase and telophase. {ECO:0000269|PubMed:29357359}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O95235-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O95235-2; Sequence=VSP_056007;
CC -!- PTM: Phosphorylated by PLK1 at Ser-528 during mitosis, creating a
CC docking site for PLK1 and recruiting PLK1 at central spindle.
CC {ECO:0000269|PubMed:12939256}.
CC -!- DISEASE: Cardiomyopathy, familial restrictive 6 (RCM6) [MIM:619433]: A
CC heart disorder characterized by impaired filling of the ventricles with
CC reduced diastolic volume, in the presence of normal or near normal wall
CC thickness and systolic function. RCM6 is an autosomal recessive, severe
CC form characterized by prenatal onset, irreversible heart failure and
CC early death. {ECO:0000269|PubMed:29357359}. Note=The disease is caused
CC by variants affecting the gene represented in this entry.
CC -!- SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase
CC superfamily. Kinesin family. {ECO:0000255|PROSITE-ProRule:PRU00283}.
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DR EMBL; AF070672; AAC83230.1; -; mRNA.
DR EMBL; AF153329; AAD37806.1; -; mRNA.
DR EMBL; AK296879; BAG59441.1; -; mRNA.
DR EMBL; AC106752; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC109442; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471062; EAW62157.1; -; Genomic_DNA.
DR EMBL; CH471062; EAW62158.1; -; Genomic_DNA.
DR EMBL; BC012999; AAH12999.1; -; mRNA.
DR CCDS; CCDS4199.1; -. [O95235-1]
DR RefSeq; NP_005724.1; NM_005733.2. [O95235-1]
DR PDB; 6YIP; X-ray; 1.43 A; A/B=596-668.
DR PDBsum; 6YIP; -.
DR AlphaFoldDB; O95235; -.
DR SMR; O95235; -.
DR BioGRID; 115418; 1041.
DR IntAct; O95235; 43.
DR MINT; O95235; -.
DR STRING; 9606.ENSP00000378356; -.
DR BindingDB; O95235; -.
DR ChEMBL; CHEMBL2021753; -.
DR iPTMnet; O95235; -.
DR PhosphoSitePlus; O95235; -.
DR BioMuta; KIF20A; -.
DR EPD; O95235; -.
DR jPOST; O95235; -.
DR MassIVE; O95235; -.
DR MaxQB; O95235; -.
DR PaxDb; O95235; -.
DR PeptideAtlas; O95235; -.
DR PRIDE; O95235; -.
DR ProteomicsDB; 4513; -.
DR ProteomicsDB; 50732; -. [O95235-1]
DR Antibodypedia; 26611; 322 antibodies from 28 providers.
DR DNASU; 10112; -.
DR Ensembl; ENST00000394894.8; ENSP00000378356.3; ENSG00000112984.12. [O95235-1]
DR Ensembl; ENST00000508792.5; ENSP00000420880.1; ENSG00000112984.12. [O95235-2]
DR GeneID; 10112; -.
DR KEGG; hsa:10112; -.
DR MANE-Select; ENST00000394894.8; ENSP00000378356.3; NM_005733.3; NP_005724.1.
DR UCSC; uc003lcj.4; human. [O95235-1]
DR CTD; 10112; -.
DR DisGeNET; 10112; -.
DR GeneCards; KIF20A; -.
DR HGNC; HGNC:9787; KIF20A.
DR HPA; ENSG00000112984; Tissue enhanced (bone marrow, lymphoid tissue, testis).
DR MalaCards; KIF20A; -.
DR MIM; 605664; gene.
DR MIM; 619433; phenotype.
DR neXtProt; NX_O95235; -.
DR OpenTargets; ENSG00000112984; -.
DR Orphanet; 75249; Familial isolated restrictive cardiomyopathy.
DR PharmGKB; PA34149; -.
DR VEuPathDB; HostDB:ENSG00000112984; -.
DR eggNOG; KOG0247; Eukaryota.
DR GeneTree; ENSGT00940000156931; -.
DR HOGENOM; CLU_001485_2_5_1; -.
DR InParanoid; O95235; -.
DR OMA; DVREECW; -.
DR OrthoDB; 314538at2759; -.
DR PhylomeDB; O95235; -.
DR TreeFam; TF105232; -.
DR BRENDA; 5.6.1.3; 2681.
DR PathwayCommons; O95235; -.
DR Reactome; R-HSA-2132295; MHC class II antigen presentation.
DR Reactome; R-HSA-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR Reactome; R-HSA-68884; Mitotic Telophase/Cytokinesis.
DR Reactome; R-HSA-983189; Kinesins.
DR SignaLink; O95235; -.
DR SIGNOR; O95235; -.
DR BioGRID-ORCS; 10112; 281 hits in 1090 CRISPR screens.
DR ChiTaRS; KIF20A; human.
DR GeneWiki; KIF20A; -.
DR GenomeRNAi; 10112; -.
DR Pharos; O95235; Tchem.
DR PRO; PR:O95235; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; O95235; protein.
DR Bgee; ENSG00000112984; Expressed in ventricular zone and 124 other tissues.
DR ExpressionAtlas; O95235; baseline and differential.
DR Genevisible; O95235; HS.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0045171; C:intercellular bridge; IDA:HPA.
DR GO; GO:0005871; C:kinesin complex; IBA:GO_Central.
DR GO; GO:0005874; C:microtubule; IBA:GO_Central.
DR GO; GO:0030496; C:midbody; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005819; C:spindle; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR GO; GO:0003777; F:microtubule motor activity; IBA:GO_Central.
DR GO; GO:0019901; F:protein kinase binding; IPI:UniProtKB.
DR GO; GO:0001578; P:microtubule bundle formation; IDA:UniProtKB.
DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR GO; GO:0061952; P:midbody abscission; IMP:UniProtKB.
DR GO; GO:0000281; P:mitotic cytokinesis; IMP:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0032465; P:regulation of cytokinesis; IDA:UniProtKB.
DR Gene3D; 3.40.850.10; -; 1.
DR InterPro; IPR027326; KIF20A.
DR InterPro; IPR027640; Kinesin-like_fam.
DR InterPro; IPR019821; Kinesin_motor_CS.
DR InterPro; IPR001752; Kinesin_motor_dom.
DR InterPro; IPR036961; Kinesin_motor_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR24115; PTHR24115; 1.
DR PANTHER; PTHR24115:SF352; PTHR24115:SF352; 1.
DR Pfam; PF00225; Kinesin; 1.
DR PRINTS; PR00380; KINESINHEAVY.
DR SMART; SM00129; KISc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00411; KINESIN_MOTOR_1; 1.
DR PROSITE; PS50067; KINESIN_MOTOR_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Alternative splicing; ATP-binding;
KW Cardiomyopathy; Coiled coil; Cytoplasm; Cytoskeleton; Disease variant;
KW Golgi apparatus; Microtubule; Motor protein; Nucleotide-binding;
KW Phosphoprotein; Protein transport; Reference proteome; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:20068231"
FT CHAIN 2..890
FT /note="Kinesin-like protein KIF20A"
FT /id="PRO_0000125460"
FT DOMAIN 64..507
FT /note="Kinesin motor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
FT REGION 763..890
FT /note="Globular"
FT /evidence="ECO:0000255"
FT REGION 832..865
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 611..762
FT /evidence="ECO:0000255"
FT BINDING 160..167
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0007744|PubMed:20068231"
FT MOD_RES 7
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:20068231"
FT MOD_RES 14
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:20068231"
FT MOD_RES 21
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:20068231"
FT MOD_RES 528
FT /note="Phosphoserine; by PLK1"
FT /evidence="ECO:0000269|PubMed:12939256,
FT ECO:0007744|PubMed:18669648"
FT MOD_RES 532
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692,
FT ECO:0007744|PubMed:23186163"
FT MOD_RES 662
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:12939256"
FT MOD_RES 668
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:12939256"
FT MOD_RES 685
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 825
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:20068231"
FT MOD_RES 857
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT MOD_RES 867
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT MOD_RES 878
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 883
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VAR_SEQ 65..82
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_056007"
FT VARIANT 63
FT /note="E -> K (in dbSNP:rs3734116)"
FT /id="VAR_049704"
FT VARIANT 182
FT /note="R -> W (in RCM6; loss-of-function variant unable to
FT rescue cardiac defects in zebrafish morphants; results in
FT greatly reduced microtubule activated motor activity; does
FT not localize to the spindle midzone)"
FT /evidence="ECO:0000269|PubMed:29357359"
FT /id="VAR_086018"
FT VARIANT 839
FT /note="P -> L (in dbSNP:rs3172747)"
FT /id="VAR_049705"
FT MUTAGEN 528
FT /note="S->A: Impairs phosphorylation by PLK1 and
FT recruitment of PLK1 to the spindle."
FT /evidence="ECO:0000269|PubMed:12939256"
FT HELIX 596..663
FT /evidence="ECO:0007829|PDB:6YIP"
SQ SEQUENCE 890 AA; 100278 MW; 6620264615496051 CRC64;
MSQGILSPPA GLLSDDDVVV SPMFESTAAD LGSVVRKNLL SDCSVVSTSL EDKQQVPSED
SMEKVKVYLR VRPLLPSELE RQEDQGCVRI ENVETLVLQA PKDSFALKSN ERGIGQATHR
FTFSQIFGPE VGQASFFNLT VKEMVKDVLK GQNWLIYTYG VTNSGKTHTI QGTIKDGGIL
PRSLALIFNS LQGQLHPTPD LKPLLSNEVI WLDSKQIRQE EMKKLSLLNG GLQEEELSTS
LKRSVYIESR IGTSTSFDSG IAGLSSISQC TSSSQLDETS HRWAQPDTAP LPVPANIRFS
IWISFFEIYN ELLYDLLEPP SQQRKRQTLR LCEDQNGNPY VKDLNWIHVQ DAEEAWKLLK
VGRKNQSFAS THLNQNSSRS HSIFSIRILH LQGEGDIVPK ISELSLCDLA GSERCKDQKS
GERLKEAGNI NTSLHTLGRC IAALRQNQQN RSKQNLVPFR DSKLTRVFQG FFTGRGRSCM
IVNVNPCAST YDETLHVAKF SAIASQLVHA PPMQLGFPSL HSFIKEHSLQ VSPSLEKGAK
ADTGLDDDIE NEADISMYGK EELLQVVEAM KTLLLKERQE KLQLEMHLRD EICNEMVEQM
QQREQWCSEH LDTQKELLEE MYEEKLNILK ESLTSFYQEE IQERDEKIEE LEALLQEARQ
QSVAHQQSGS ELALRRSQRL AASASTQQLQ EVKAKLQQCK AELNSTTEEL HKYQKMLEPP
PSAKPFTIDV DKKLEEGQKN IRLLRTELQK LGESLQSAER ACCHSTGAGK LRQALTTCDD
ILIKQDQTLA ELQNNMVLVK LDLRKKAACI AEQYHTVLKL QGQVSAKKRL GTNQENQQPN
QQPPGKKPFL RNLLPRTPTC QSSTDCSPYA RILRSRRSPL LKSGPFGKKY
