KIB1_ARATH
ID KIB1_ARATH Reviewed; 382 AA.
AC Q9SU05;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=F-box/kelch-repeat protein KIB1 {ECO:0000303|PubMed:28575660};
DE AltName: Full=Protein KINK SUPPRESSED IN BZR1-1D 1 {ECO:0000303|PubMed:28575660};
GN Name=KIB1 {ECO:0000303|PubMed:28575660};
GN OrderedLocusNames=At4g12810 {ECO:0000312|Araport:AT4G12810};
GN ORFNames=T20K18.160 {ECO:0000312|EMBL:CAB40998.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH SKP1A/ASK1; ASK1/SK11;
RP ASK3/SK12; ASK5/SK13; ASK7/BIN2/SK21; ASK9/SK22 AND ASK6/SK23, TISSUE
RP SPECIFICITY, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia, and cv. Wassilewskija;
RX PubMed=28575660; DOI=10.1016/j.molcel.2017.05.012;
RA Zhu J.-Y., Li Y., Cao D.-M., Yang H., Oh E., Bi Y., Zhu S., Wang Z.-Y.;
RT "The F-box protein KIB1 mediates brassinosteroid-induced inactivation and
RT degradation of GSK3-like kinases in Arabidopsis.";
RL Mol. Cell 66:648-657(2017).
CC -!- FUNCTION: Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase
CC complexes, which may mediate the ubiquitination and subsequent
CC proteasomal degradation of target proteins. Required for
CC brassinosteroid (BR) signal transduction. Mediates ASK7/BIN2/SK21
CC inactivation both by competing with substrate binding (e.g. BZR1) and
CC by promoting its ubiquitination and subsequent proteasomal degradation.
CC {ECO:0000269|PubMed:28575660}.
CC -!- SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex.
CC Binds directly to several GSK3 family proteins such as SKP1A/ASK1,
CC ASK1/SK11, ASK3/SK12, ASK5/SK13, ASK7/BIN2/SK21, ASK9/SK22 and
CC ASK6/SK23. Interacts with ASK7/BIN2/SK21 in a brassinosteroid (BR)-
CC dependent manner. {ECO:0000269|PubMed:28575660}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:28575660}. Nucleus,
CC nucleolus {ECO:0000269|PubMed:28575660}. Note=Mostly localized in the
CC nucleolus, and, to a lower extent, in the cytoplasm.
CC {ECO:0000269|PubMed:28575660}.
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, leaves, stems, flower buds
CC and flowers. {ECO:0000269|PubMed:28575660}.
CC -!- DISRUPTION PHENOTYPE: Abolished brassinosteroid (BR)-induced
CC ASK7/BIN2/SK21 degradation, and severe BR-insensitivity. Suppression of
CC the constitutive BR-response phenotype in the dominant mutant bzr1-1D,
CC and accumulation of phosphorylated BZR1. {ECO:0000269|PubMed:28575660}.
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DR EMBL; AL049640; CAB40998.1; -; Genomic_DNA.
DR EMBL; AL161534; CAB78323.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE83187.1; -; Genomic_DNA.
DR PIR; T06639; T06639.
DR RefSeq; NP_193017.1; NM_117350.1.
DR AlphaFoldDB; Q9SU05; -.
DR SMR; Q9SU05; -.
DR STRING; 3702.AT4G12810.1; -.
DR iPTMnet; Q9SU05; -.
DR PaxDb; Q9SU05; -.
DR PRIDE; Q9SU05; -.
DR EnsemblPlants; AT4G12810.1; AT4G12810.1; AT4G12810.
DR GeneID; 826893; -.
DR Gramene; AT4G12810.1; AT4G12810.1; AT4G12810.
DR KEGG; ath:AT4G12810; -.
DR Araport; AT4G12810; -.
DR TAIR; locus:2135823; AT4G12810.
DR eggNOG; ENOG502S0MJ; Eukaryota.
DR HOGENOM; CLU_019286_7_1_1; -.
DR InParanoid; Q9SU05; -.
DR OMA; HFRSANV; -.
DR OrthoDB; 820958at2759; -.
DR PhylomeDB; Q9SU05; -.
DR PRO; PR:Q9SU05; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SU05; baseline.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005730; C:nucleolus; IDA:UniProtKB.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; IPI:TAIR.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IDA:TAIR.
DR GO; GO:0009742; P:brassinosteroid mediated signaling pathway; IGI:TAIR.
DR GO; GO:1900459; P:positive regulation of brassinosteroid mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; IDA:TAIR.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IDA:UniProtKB.
DR InterPro; IPR005174; DUF295.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR011043; Gal_Oxase/kelch_b-propeller.
DR Pfam; PF03478; DUF295; 1.
DR Pfam; PF00646; F-box; 1.
DR SUPFAM; SSF50965; SSF50965; 1.
PE 1: Evidence at protein level;
KW Brassinosteroid signaling pathway; Cytoplasm; Kelch repeat; Nucleus;
KW Reference proteome; Repeat; Ubl conjugation pathway.
FT CHAIN 1..382
FT /note="F-box/kelch-repeat protein KIB1"
FT /id="PRO_0000283242"
FT DOMAIN 22..69
FT /note="F-box"
FT /evidence="ECO:0000255"
FT REPEAT 73..117
FT /note="Kelch 1"
FT /evidence="ECO:0000255"
FT REPEAT 159..209
FT /note="Kelch 2"
FT /evidence="ECO:0000255"
FT REPEAT 259..306
FT /note="Kelch 3"
FT /evidence="ECO:0000255"
SQ SEQUENCE 382 AA; 44034 MW; 6CB0D36BC3CAC404 CRC64;
MTHKKQKKEM SDSEKKTFNE DSKHSILAVD LVRLILERLS FVDFHRARCV SSIWYIASKT
VIGVTNPTTP WLILFPKGDV EIKKDSCKLY DPHENKTYIV RDLGFDLVTS RCLASSGSWF
LMLDHRTEFH LLNLFTRVRI PLPSLESTRG SDIKIGNAVL WVDEQRKDYL VVWNISSLFG
YHKKGDDRWK VFKPLENERC IIAMVFKENK LYVLSVDGNV DVFYFSGNDS PVRCATLPSS
PLRKGHKVVV TLSGEVLIIV AKVEPYPRTR LCFFAVYKMD PKSSRWETIK SLAGEALILD
LGITVEAKVM KNCIYFSNDQ FHRYNENSLW NVSNKSGVFV YHFRSANVVQ LVELLTASSR
TSKILFKDAR CFFPTFTSKW LL
