ID   KIB2_ARATH              Reviewed;         442 AA.
AC   Q9SU04;
DT   10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=F-box protein KIB2 {ECO:0000303|PubMed:28575660};
DE   AltName: Full=Protein KINK SUPPRESSED IN BZR1-1D 2 {ECO:0000303|PubMed:28575660};
GN   Name=KIB2 {ECO:0000303|PubMed:28575660};
GN   OrderedLocusNames=At4g12820 {ECO:0000312|Araport:AT4G12820};
GN   ORFNames=T20K18.170 {ECO:0000312|EMBL:CAB40999.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA   Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA   Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA   de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA   Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA   Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA   Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA   Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA   Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA   Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA   Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA   Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA   Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA   Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA   Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA   Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA   Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA   Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA   Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA   Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA   Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA   Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA   Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA   de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA   Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA   Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA   Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA   Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA   Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA   Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA   Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA   O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA   Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA   Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL   Nature 402:769-777(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION WITH ASK7/BIN2/SK21.
RC   STRAIN=cv. Columbia, and cv. Wassilewskija;
RX   PubMed=28575660; DOI=10.1016/j.molcel.2017.05.012;
RA   Zhu J.-Y., Li Y., Cao D.-M., Yang H., Oh E., Bi Y., Zhu S., Wang Z.-Y.;
RT   "The F-box protein KIB1 mediates brassinosteroid-induced inactivation and
RT   degradation of GSK3-like kinases in Arabidopsis.";
RL   Mol. Cell 66:648-657(2017).
CC   -!- FUNCTION: Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase
CC       complexes, which may mediate the ubiquitination and subsequent
CC       proteasomal degradation of target proteins. Required for
CC       brassinosteroid (BR) signal transduction. Mediates ASK7/BIN2/SK21
CC       inactivation both by competing with substrate binding (e.g. BZR1) and
CC       by promoting its ubiquitination and subsequent proteasomal degradation.
CC       {ECO:0000269|PubMed:28575660}.
CC   -!- SUBUNIT: Interacts with ASK7/BIN2/SK21. {ECO:0000269|PubMed:28575660}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9SU05}.
CC       Nucleus, nucleolus {ECO:0000250|UniProtKB:Q9SU05}. Nucleus
CC       {ECO:0000255|PROSITE-ProRule:PRU00768}.
CC   -!- DISRUPTION PHENOTYPE: Abolished brassinosteroid (BR)-induced
CC       ASK7/BIN2/SK21 degradation, and BR-insensitivity. Suppression of the
CC       constitutive BR-response phenotype in the dominant mutant bzr1-1D, and
CC       accumulation of phosphorylated BZR1. {ECO:0000269|PubMed:28575660}.
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DR   EMBL; AL049640; CAB40999.1; -; Genomic_DNA.
DR   EMBL; AL161535; CAB78324.1; -; Genomic_DNA.
DR   EMBL; CP002687; AEE83188.1; -; Genomic_DNA.
DR   PIR; T06640; T06640.
DR   RefSeq; NP_193018.1; NM_117351.2.
DR   AlphaFoldDB; Q9SU04; -.
DR   STRING; 3702.AT4G12820.1; -.
DR   PaxDb; Q9SU04; -.
DR   PRIDE; Q9SU04; -.
DR   DNASU; 826894; -.
DR   EnsemblPlants; AT4G12820.1; AT4G12820.1; AT4G12820.
DR   GeneID; 826894; -.
DR   Gramene; AT4G12820.1; AT4G12820.1; AT4G12820.
DR   KEGG; ath:AT4G12820; -.
DR   Araport; AT4G12820; -.
DR   TAIR; locus:2135833; AT4G12820.
DR   eggNOG; ENOG502S0MJ; Eukaryota.
DR   HOGENOM; CLU_019286_7_1_1; -.
DR   InParanoid; Q9SU04; -.
DR   OMA; YSASESC; -.
DR   OrthoDB; 820958at2759; -.
DR   PhylomeDB; Q9SU04; -.
DR   PRO; PR:Q9SU04; -.
DR   Proteomes; UP000006548; Chromosome 4.
DR   ExpressionAtlas; Q9SU04; baseline.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR   GO; GO:0009742; P:brassinosteroid mediated signaling pathway; IGI:TAIR.
DR   GO; GO:1900459; P:positive regulation of brassinosteroid mediated signaling pathway; IMP:UniProtKB.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IDA:UniProtKB.
DR   InterPro; IPR005174; DUF295.
DR   Pfam; PF03478; DUF295; 1.
PE   1: Evidence at protein level;
KW   Brassinosteroid signaling pathway; Cytoplasm; Nucleus; Reference proteome;
KW   Repeat; Ubl conjugation pathway.
FT   CHAIN           1..442
FT                   /note="F-box protein KIB2"
FT                   /id="PRO_0000396071"
FT   DOMAIN          62..109
FT                   /note="F-box"
FT                   /evidence="ECO:0000255"
FT   MOTIF           236..243
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
SQ   SEQUENCE   442 AA;  50997 MW;  32B8D44A1078FFFB CRC64;
     MAPLNSQAAG EEESNYQCRF PRFMHLSWKR PYLTRVFDLA VIQKKQKKEM SDSEEKKCND
     DSKQPVLVLD LLRSILERLS FVDFHRGRCI SLEWYSASES CLAVKNPTSP WIILFPSENV
     ESKNDSCKLY NPRDHSSYIV RDLGFDLARS RCLASSGSWF LMLDHRTDFH ILNLFTRVRI
     PLPPLESTGD GNVFKRVYEN VSDGSCIKID NAVLWVDEKR RDYLVVWNIS RLFGYHKKGD
     ENYSWKVFKP LKKNDCCIDM AFKENKLYVL SVTRKVTVYD FSGGDSPVKC ATFPSLRFRN
     GYSYNTQGCH YKVAVTLSGE VLIIVAKVEP YPRVECFFAV YKMDHNSSGY ERISLGGEAL
     LLDLGITVEA NCMKNCIYFS NDQFHRYNGK SLCDDSNKNG ICVYHIRSRY VVQVFEHLTA
     SSKIAFKDAR SFFPIFGGKW LL