KIF12_HUMAN
ID KIF12_HUMAN Reviewed; 646 AA.
AC Q96FN5; Q5TBE0;
DT 11-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2006, sequence version 3.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Kinesin-like protein KIF12;
GN Name=KIF12;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 96-646.
RC TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=15643526;
RA Katoh M., Katoh M.;
RT "Characterization of KIF12 gene in silico.";
RL Oncol. Rep. 13:367-370(2005).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-6; SER-14 AND SER-638, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in fetal liver, adult brain and
CC pancreatic islet as well as in kidney tumors, uterus cancer and
CC pancreatic cancer. {ECO:0000269|PubMed:15643526}.
CC -!- SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase
CC superfamily. Kinesin family. {ECO:0000255|PROSITE-ProRule:PRU00283}.
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DR EMBL; AL137850; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC010626; AAH10626.1; -; mRNA.
DR RefSeq; NP_612433.1; NM_138424.1.
DR AlphaFoldDB; Q96FN5; -.
DR SMR; Q96FN5; -.
DR BioGRID; 125234; 4.
DR IntAct; Q96FN5; 3.
DR STRING; 9606.ENSP00000363232; -.
DR iPTMnet; Q96FN5; -.
DR PhosphoSitePlus; Q96FN5; -.
DR BioMuta; KIF12; -.
DR DMDM; 85541032; -.
DR jPOST; Q96FN5; -.
DR MassIVE; Q96FN5; -.
DR PaxDb; Q96FN5; -.
DR PeptideAtlas; Q96FN5; -.
DR PRIDE; Q96FN5; -.
DR ProteomicsDB; 76547; -.
DR Antibodypedia; 15422; 77 antibodies from 21 providers.
DR DNASU; 113220; -.
DR GeneID; 113220; -.
DR KEGG; hsa:113220; -.
DR CTD; 113220; -.
DR DisGeNET; 113220; -.
DR GeneCards; KIF12; -.
DR HGNC; HGNC:21495; KIF12.
DR HPA; ENSG00000136883; Tissue enhanced (kidney, pancreas).
DR MIM; 611278; gene.
DR neXtProt; NX_Q96FN5; -.
DR PharmGKB; PA134931618; -.
DR VEuPathDB; HostDB:ENSG00000136883; -.
DR eggNOG; KOG4280; Eukaryota.
DR InParanoid; Q96FN5; -.
DR OrthoDB; 1132313at2759; -.
DR PhylomeDB; Q96FN5; -.
DR PathwayCommons; Q96FN5; -.
DR Reactome; R-HSA-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR Reactome; R-HSA-983189; Kinesins.
DR SignaLink; Q96FN5; -.
DR BioGRID-ORCS; 113220; 13 hits in 1072 CRISPR screens.
DR ChiTaRS; KIF12; human.
DR GenomeRNAi; 113220; -.
DR Pharos; Q96FN5; Tdark.
DR PRO; PR:Q96FN5; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; Q96FN5; protein.
DR Bgee; ENSG00000136883; Expressed in metanephros cortex and 133 other tissues.
DR ExpressionAtlas; Q96FN5; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0070062; C:extracellular exosome; IDA:UniProtKB.
DR GO; GO:0005871; C:kinesin complex; IBA:GO_Central.
DR GO; GO:0005874; C:microtubule; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR GO; GO:0003777; F:microtubule motor activity; IBA:GO_Central.
DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR Gene3D; 3.40.850.10; -; 1.
DR InterPro; IPR028763; Kif12.
DR InterPro; IPR027640; Kinesin-like_fam.
DR InterPro; IPR019821; Kinesin_motor_CS.
DR InterPro; IPR001752; Kinesin_motor_dom.
DR InterPro; IPR036961; Kinesin_motor_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR24115; PTHR24115; 1.
DR PANTHER; PTHR24115:SF418; PTHR24115:SF418; 1.
DR Pfam; PF00225; Kinesin; 1.
DR PRINTS; PR00380; KINESINHEAVY.
DR SMART; SM00129; KISc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00411; KINESIN_MOTOR_1; 1.
DR PROSITE; PS50067; KINESIN_MOTOR_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Coiled coil; Cytoplasm; Cytoskeleton; Microtubule;
KW Motor protein; Nucleotide-binding; Phosphoprotein; Reference proteome.
FT CHAIN 1..646
FT /note="Kinesin-like protein KIF12"
FT /id="PRO_0000125444"
FT DOMAIN 25..360
FT /note="Kinesin motor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 529..565
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 579..646
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 372..467
FT /evidence="ECO:0000255"
FT COMPBIAS 537..552
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 104..111
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
FT MOD_RES 6
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MOD_RES 14
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MOD_RES 369
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9D2Z8"
FT MOD_RES 638
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
SQ SEQUENCE 646 AA; 70660 MW; 0DC0A9732D7BB15A CRC64;
MEERGSPDGD LARSLEQGPE GPETPIQVVL RVRPMSAAEL RRGQQSVLHC SGTRTLQGGP
EVAFRFGAVL DAARTQEDVF RACGVRRLGE LALRGFSCTV FTFGQTGSGK TYTLTGPPPQ
GEGVPVPPSL AGIMQRTFAW LLDRVQHLGA PVTLRASYLE IYNEQVRDLL SLGSPRPLPV
RWNKTRGFYV EQLRVVEFGS LEALMELLQT GLSRRRNSAH TLNQASSRSH ALLTLYISRQ
TAQQMPSVDP GEPPVGGKLC FVDLAGSEKV AATGSRGELM LEANSINRSL LALGHCISLL
LDPQRKQSHI PFRDSKLTKL LADSLGGRGV TLMVACVSPS AQCLPETLST LRYASRAQRV
TTRPQAPKSP VAKQPQRLET EMLQLQEENR RLQFQLDQMD CKASGLSGAR VAWAQRNLYG
MLQEFMLENE RLRKEKSQLQ NSRDLAQNEQ RILAQQVHAL ERRLLSACYH HQQGPGLTPP
CPCLMAPAPP CHALPPLYSC PCCHICPLCR VPLAHWACLP GEHHLPQVLD PEASGGRPPS
ARPPPWAPPC SPGSAKCPRE RSHSDWTQTR VLAEMLTEEE VVPSAPPLPV RPPKTSPGLR
GGAGVPNLAQ RLEALRDQIG SSLRRGRSQP PCSEGARSPG QVLPPH
