KIF25_HUMAN
ID KIF25_HUMAN Reviewed; 384 AA.
AC Q9UIL4; A8K0C3; O94775; Q5SZU9;
DT 23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 2.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=Kinesin-like protein KIF25 {ECO:0000305};
DE AltName: Full=Kinesin-like protein 3 {ECO:0000303|PubMed:9925916};
GN Name=KIF25 {ECO:0000312|HGNC:HGNC:6390};
GN Synonyms=KNSL3 {ECO:0000303|PubMed:9925916};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], ALTERNATIVE SPLICING, AND VARIANT
RP MET-28.
RX PubMed=9925916; DOI=10.1159/000015159;
RA Okamoto S., Matsushima M., Nakamura Y.;
RT "Identification, genomic organization, and alternative splicing of KNSL3, a
RT novel human gene encoding a kinesin-like protein.";
RL Cytogenet. Cell Genet. 83:25-29(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Cerebellum;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [4]
RP FUNCTION.
RX PubMed=22354037; DOI=10.1038/emboj.2012.36;
RA McKnight N.C., Jefferies H.B., Alemu E.A., Saunders R.E., Howell M.,
RA Johansen T., Tooze S.A.;
RT "Genome-wide siRNA screen reveals amino acid starvation-induced autophagy
RT requires SCOC and WAC.";
RL EMBO J. 31:1931-1946(2012).
RN [5]
RP FUNCTION.
RX PubMed=28263957; DOI=10.1038/ncb3486;
RA Decarreau J., Wagenbach M., Lynch E., Halpern A.R., Vaughan J.C.,
RA Kollman J., Wordeman L.;
RT "The tetrameric kinesin Kif25 suppresses pre-mitotic centrosome separation
RT to establish proper spindle orientation.";
RL Nat. Cell Biol. 19:384-390(2017).
CC -!- FUNCTION: Minus-end microtubule-dependent motor protein (By
CC similarity). Acts as a negative regulator of centrosome separation
CC required to prevent premature centrosome separation during interphase
CC (PubMed:28263957). Required to maintain a centered nucleus to ensure
CC that the spindle is stably oriented at the onset of mitosis
CC (PubMed:28263957). May also act as a negative regulator of amino acid
CC starvation-induced autophagy (PubMed:22354037).
CC {ECO:0000250|UniProtKB:Q4R918, ECO:0000269|PubMed:22354037,
CC ECO:0000269|PubMed:28263957}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:Q4R918}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000250|UniProtKB:Q4R918}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9UIL4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9UIL4-2; Sequence=VSP_002867;
CC -!- SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase
CC superfamily. Kinesin family. {ECO:0000255|PROSITE-ProRule:PRU00283}.
CC -!- CAUTION: In contrast to the ortholog protein in primates, human KIF25
CC protein is shorter at the N-terminus. While the kinesin motor domain is
CC intact, it is unknown whether the absence of the N-terminus affects the
CC microtubule-dependent motor activity. {ECO:0000305|PubMed:28263957}.
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DR EMBL; AB012722; BAA36417.1; -; mRNA.
DR EMBL; AK289488; BAF82177.1; -; mRNA.
DR EMBL; AB012723; BAA36418.1; -; Genomic_DNA.
DR EMBL; AL589733; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS43530.1; -. [Q9UIL4-2]
DR CCDS; CCDS5305.1; -. [Q9UIL4-1]
DR RefSeq; NP_005346.3; NM_005355.3. [Q9UIL4-2]
DR RefSeq; NP_085118.2; NM_030615.2. [Q9UIL4-1]
DR RefSeq; XP_011534104.1; XM_011535802.2. [Q9UIL4-1]
DR RefSeq; XP_011534105.1; XM_011535803.2. [Q9UIL4-2]
DR AlphaFoldDB; Q9UIL4; -.
DR SMR; Q9UIL4; -.
DR BioGRID; 110032; 1.
DR IntAct; Q9UIL4; 1.
DR STRING; 9606.ENSP00000388878; -.
DR iPTMnet; Q9UIL4; -.
DR PhosphoSitePlus; Q9UIL4; -.
DR BioMuta; KIF25; -.
DR DMDM; 20138788; -.
DR MassIVE; Q9UIL4; -.
DR PaxDb; Q9UIL4; -.
DR PeptideAtlas; Q9UIL4; -.
DR PRIDE; Q9UIL4; -.
DR Antibodypedia; 20075; 293 antibodies from 19 providers.
DR DNASU; 3834; -.
DR Ensembl; ENST00000443060.6; ENSP00000388878.2; ENSG00000125337.21. [Q9UIL4-1]
DR Ensembl; ENST00000643607.3; ENSP00000496229.1; ENSG00000125337.21. [Q9UIL4-1]
DR GeneID; 3834; -.
DR KEGG; hsa:3834; -.
DR MANE-Select; ENST00000643607.3; ENSP00000496229.1; NM_030615.4; NP_085118.2.
DR UCSC; uc003qwk.1; human. [Q9UIL4-1]
DR CTD; 3834; -.
DR DisGeNET; 3834; -.
DR GeneCards; KIF25; -.
DR HGNC; HGNC:6390; KIF25.
DR HPA; ENSG00000125337; Tissue enhanced (brain, retina).
DR MIM; 603815; gene.
DR neXtProt; NX_Q9UIL4; -.
DR OpenTargets; ENSG00000125337; -.
DR PharmGKB; PA30179; -.
DR VEuPathDB; HostDB:ENSG00000125337; -.
DR eggNOG; KOG0239; Eukaryota.
DR GeneTree; ENSGT00940000162166; -.
DR HOGENOM; CLU_001485_2_2_1; -.
DR InParanoid; Q9UIL4; -.
DR OMA; GQKYLTE; -.
DR OrthoDB; 364605at2759; -.
DR PhylomeDB; Q9UIL4; -.
DR TreeFam; TF105234; -.
DR PathwayCommons; Q9UIL4; -.
DR Reactome; R-HSA-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR Reactome; R-HSA-983189; Kinesins.
DR SignaLink; Q9UIL4; -.
DR BioGRID-ORCS; 3834; 13 hits in 1075 CRISPR screens.
DR GenomeRNAi; 3834; -.
DR Pharos; Q9UIL4; Tbio.
DR PRO; PR:Q9UIL4; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; Q9UIL4; protein.
DR Bgee; ENSG00000125337; Expressed in buccal mucosa cell and 104 other tissues.
DR ExpressionAtlas; Q9UIL4; baseline and differential.
DR Genevisible; Q9UIL4; HS.
DR GO; GO:0005813; C:centrosome; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005871; C:kinesin complex; IBA:GO_Central.
DR GO; GO:0005874; C:microtubule; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR GO; GO:0003777; F:microtubule motor activity; IBA:GO_Central.
DR GO; GO:0008569; F:minus-end-directed microtubule motor activity; ISS:UniProtKB.
DR GO; GO:0051294; P:establishment of spindle orientation; IDA:UniProtKB.
DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; TAS:ProtInc.
DR GO; GO:0010507; P:negative regulation of autophagy; IMP:BHF-UCL.
DR GO; GO:0046603; P:negative regulation of mitotic centrosome separation; IDA:UniProtKB.
DR GO; GO:0051647; P:nucleus localization; IDA:UniProtKB.
DR GO; GO:0006996; P:organelle organization; TAS:ProtInc.
DR GO; GO:0051289; P:protein homotetramerization; ISS:UniProtKB.
DR Gene3D; 3.40.850.10; -; 1.
DR InterPro; IPR027640; Kinesin-like_fam.
DR InterPro; IPR019821; Kinesin_motor_CS.
DR InterPro; IPR001752; Kinesin_motor_dom.
DR InterPro; IPR036961; Kinesin_motor_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR24115; PTHR24115; 1.
DR Pfam; PF00225; Kinesin; 1.
DR PRINTS; PR00380; KINESINHEAVY.
DR SMART; SM00129; KISc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00411; KINESIN_MOTOR_1; 1.
DR PROSITE; PS50067; KINESIN_MOTOR_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Cytoplasm; Cytoskeleton; Microtubule;
KW Motor protein; Nucleotide-binding; Reference proteome.
FT CHAIN 1..384
FT /note="Kinesin-like protein KIF25"
FT /id="PRO_0000125435"
FT DOMAIN 7..363
FT /note="Kinesin motor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 217..256
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 362..384
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
FT VAR_SEQ 278..329
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_002867"
FT VARIANT 28
FT /note="K -> M (in dbSNP:rs4708626)"
FT /evidence="ECO:0000269|PubMed:9925916"
FT /id="VAR_059369"
FT VARIANT 41
FT /note="A -> T (in dbSNP:rs34049091)"
FT /id="VAR_061280"
FT VARIANT 229
FT /note="T -> P (in dbSNP:rs12197062)"
FT /id="VAR_049687"
FT VARIANT 255
FT /note="A -> T (in dbSNP:rs2073634)"
FT /id="VAR_049688"
FT CONFLICT 185
FT /note="A -> T (in Ref. 2; BAF82177)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 384 AA; 40686 MW; 7739D463BCFDB429 CRC64;
MTWTSGQLQR EKQARPGSGA VLAFPDDKDL RVYGPAESQS AVFGDVCPLL TSLLDGYNVC
VMAYGQTGSG KSYTMLGRHS DDGPVLPLDP QSDLGIIPRV AEELFRLILE NTSRSPKVEV
SIVEVYNNDI FDLLAKDSIA AVSGVKREVV TAKDGRTEVA LLASEAVGSA SKLMELVHGG
LQLRAKHPTL VHADSSRSHL IITVTLTTAS CSDSTADQAC SATLPREQTE AGRAGRSRRA
SQGALAPQLV PGNPAGHAEQ VQARLQLVDS AGSECVGVSG VTGLALREMA CISRSLAALA
GVLGALLEHR GHAPYRNSRL THLLQDCLGG DAKLLVILCI SPSQRHLAQT LQGLGFGIRA
RQVQRGPARK KPPSSQTEGK RRPD
