KIF2B_MACFA
ID KIF2B_MACFA Reviewed; 670 AA.
AC Q95LT1; Q8WNT8;
DT 17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Kinesin-like protein KIF2B;
GN Name=KIF2B; ORFNames=QtsA-10833, QtsA-13688, QtsA-18420;
OS Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC Cercopithecidae; Cercopithecinae; Macaca.
OX NCBI_TaxID=9541;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=12498619; DOI=10.1186/1471-2164-3-36;
RA Osada N., Hida M., Kusuda J., Tanuma R., Hirata M., Suto Y., Hirai M.,
RA Terao K., Sugano S., Hashimoto K.;
RT "Cynomolgus monkey testicular cDNAs for discovery of novel human genes in
RT the human genome sequence.";
RL BMC Genomics 3:36-36(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RA Hashimoto K., Osada N., Hida M., Kusuda J., Tanuma R., Hirai M., Terao K.,
RA Sugano S.;
RT "Isolation of novel full-length cDNA clones from macaque testis cDNA
RT libraries.";
RL Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Plus end-directed microtubule-dependent motor required for
CC spindle assembly and chromosome movement during mitosis. Has
CC microtubule depolymerization activity. Plays a role in chromosome
CC congression. {ECO:0000250|UniProtKB:Q8N4N8}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000250|UniProtKB:Q8N4N8}. Cytoplasm,
CC cytoskeleton, spindle {ECO:0000250|UniProtKB:Q8N4N8}. Chromosome,
CC centromere, kinetochore {ECO:0000250|UniProtKB:Q8N4N8}.
CC Note=Association with kinetochore is transient.
CC {ECO:0000250|UniProtKB:Q8N4N8}.
CC -!- PTM: Phosphorylation at Thr-122 by PLK1 is required for activity in the
CC correction of kinetochore-microtubules attachment errors, while
CC phosphorylation at Ser-201 also by PLK1 is required for the kinetochore
CC localization and activity in prometaphase.
CC {ECO:0000250|UniProtKB:Q8N4N8}.
CC -!- SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase
CC superfamily. Kinesin family. MCAK/KIF2 subfamily. {ECO:0000255|PROSITE-
CC ProRule:PRU00283}.
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DR EMBL; AB071111; BAB64505.1; -; mRNA.
DR EMBL; AB064992; BAB83534.1; -; mRNA.
DR EMBL; AB066557; BAB84032.1; -; mRNA.
DR AlphaFoldDB; Q95LT1; -.
DR SMR; Q95LT1; -.
DR STRING; 9541.XP_005583816.1; -.
DR PRIDE; Q95LT1; -.
DR eggNOG; KOG0246; Eukaryota.
DR Proteomes; UP000233100; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008017; F:microtubule binding; IEA:InterPro.
DR GO; GO:0003777; F:microtubule motor activity; IEA:InterPro.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0051310; P:metaphase plate congression; ISS:UniProtKB.
DR GO; GO:0007019; P:microtubule depolymerization; ISS:UniProtKB.
DR GO; GO:0007018; P:microtubule-based movement; IEA:InterPro.
DR GO; GO:0051983; P:regulation of chromosome segregation; ISS:UniProtKB.
DR Gene3D; 3.40.850.10; -; 1.
DR InterPro; IPR027640; Kinesin-like_fam.
DR InterPro; IPR019821; Kinesin_motor_CS.
DR InterPro; IPR001752; Kinesin_motor_dom.
DR InterPro; IPR036961; Kinesin_motor_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR24115; PTHR24115; 1.
DR Pfam; PF00225; Kinesin; 1.
DR PRINTS; PR00380; KINESINHEAVY.
DR SMART; SM00129; KISc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00411; KINESIN_MOTOR_1; 1.
DR PROSITE; PS50067; KINESIN_MOTOR_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cell cycle; Cell division; Centromere; Chromosome;
KW Coiled coil; Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis;
KW Motor protein; Nucleotide-binding; Phosphoprotein; Reference proteome.
FT CHAIN 1..670
FT /note="Kinesin-like protein KIF2B"
FT /id="PRO_0000253716"
FT DOMAIN 210..540
FT /note="Kinesin motor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
FT COILED 146..173
FT /evidence="ECO:0000255"
FT BINDING 300..307
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
FT MOD_RES 122
FT /note="Phosphothreonine; by PLK1"
FT /evidence="ECO:0000250|UniProtKB:Q8N4N8"
FT MOD_RES 201
FT /note="Phosphoserine; by PLK1"
FT /evidence="ECO:0000250|UniProtKB:Q8N4N8"
FT CONFLICT 180
FT /note="P -> S (in Ref. 2; BAB84032)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 670 AA; 75628 MW; E1136FFC4F174BF1 CRC64;
MAGQFCLPVS PRLSPLKPLK PHFGDIQVGI YVAIQRSDKR MHLAVVTEIN RENSWVTVEW
VEKAVKKGKK IDLETILLLN PSLDSAEDPM PVSPLAPAPS SAIGDQSTTT KRVVMIPQKN
KTASGDSLDV RVPSKPCLMK QKKSPCLQEI EKVQKQREKR RRLQQEIRAR RALDVNTRNP
NYEIMHMIEE YRRHLDSSKI SVLEPRQEHR ICVCVRKRPL NQRETTLKDL DIITVPSDNV
VMVHESKQKV DLTRYLENQT FCFDHAFDDK ASNELVYQFT AQPLVESIFR KGMATCFAYG
QTGSGKTYTM GGDFAGRAQD HSKGIYALVA QDVFLLLRNS IYEKLDLKVY GTFFEIYGGK
VYDLLNWKKK LQVLEDGNQQ IQVVGLQEQE VCCVEEVLNL VELGNSCRTS RQTSVNAHSS
RSHAVFQIIL KSGGKLHGKF SLVDLAGNER GADTTKASRK RQLEGAEINK SLLALKECIL
ALGQNKPHTP FRASKLTQVL RDSFIGRNSS TCMIATISPG MTSCENTLNT LRYANRVKEL
NADGRPYHRG LYPNGHEASR MLKSHIGNSE MSLQRDEFIK IPCVQSEEQQ KEIEDVERAT
LLGKDTTTSR KGSSQWLENI QERTGGVNHD VDFCIAQSLS ILEQKIGVLT EIQKKLKLLR
ADLHVKSKVE
