KIMA_BACSU
ID KIMA_BACSU Reviewed; 607 AA.
AC P96589; Q797M3;
DT 16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Potassium transporter KimA {ECO:0000305};
DE AltName: Full=K(+) importer A {ECO:0000303|PubMed:28420751};
GN Name=kimA {ECO:0000303|PubMed:28420751}; Synonyms=ydaO;
GN OrderedLocusNames=BSU04320;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.;
RT "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus
RT subtilis genome.";
RL Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP INDUCTION.
RX PubMed=20511502; DOI=10.1128/jb.00450-10;
RA Block K.F., Hammond M.C., Breaker R.R.;
RT "Evidence for widespread gene control function by the ydaO riboswitch
RT candidate.";
RL J. Bacteriol. 192:3983-3989(2010).
RN [4]
RP INDUCTION.
RX PubMed=24141192; DOI=10.1038/nchembio.1363;
RA Nelson J.W., Sudarsan N., Furukawa K., Weinberg Z., Wang J.X.,
RA Breaker R.R.;
RT "Riboswitches in eubacteria sense the second messenger c-di-AMP.";
RL Nat. Chem. Biol. 9:834-839(2013).
RN [5]
RP FUNCTION AS A TRANSPORTER, AND INDUCTION.
RX PubMed=28420751; DOI=10.1126/scisignal.aal3011;
RA Gundlach J., Herzberg C., Kaever V., Gunka K., Hoffmann T., Weiss M.,
RA Gibhardt J., Thuermer A., Hertel D., Daniel R., Bremer E., Commichau F.M.,
RA Stuelke J.;
RT "Control of potassium homeostasis is an essential function of the second
RT messenger cyclic di-AMP in Bacillus subtilis.";
RL Sci. Signal. 10:0-0(2017).
CC -!- FUNCTION: High-affinity potassium transporter.
CC {ECO:0000269|PubMed:28420751}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Induced by low K(+) concentrations (PubMed:28420751).
CC Expression is regulated by cyclic di-AMP (c-di-AMP), via the ydaO
CC riboswitch (PubMed:20511502, PubMed:24141192, PubMed:28420751). High
CC external concentrations of K(+) stimulate an increase in the production
CC of c-di-AMP, which binds to the riboswitch and prevents kimA expression
CC (PubMed:28420751). {ECO:0000269|PubMed:20511502,
CC ECO:0000269|PubMed:24141192, ECO:0000269|PubMed:28420751}.
CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC superfamily. {ECO:0000305}.
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DR EMBL; AB001488; BAA19269.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12239.1; -; Genomic_DNA.
DR PIR; F69769; F69769.
DR RefSeq; NP_388313.1; NC_000964.3.
DR RefSeq; WP_003246693.1; NZ_JNCM01000031.1.
DR PDB; 6S3K; EM; 3.70 A; A/B=1-607.
DR PDBsum; 6S3K; -.
DR AlphaFoldDB; P96589; -.
DR SMR; P96589; -.
DR STRING; 224308.BSU04320; -.
DR PaxDb; P96589; -.
DR PRIDE; P96589; -.
DR EnsemblBacteria; CAB12239; CAB12239; BSU_04320.
DR GeneID; 938237; -.
DR KEGG; bsu:BSU04320; -.
DR PATRIC; fig|224308.179.peg.458; -.
DR eggNOG; COG0531; Bacteria.
DR InParanoid; P96589; -.
DR OMA; VGHWYEH; -.
DR PhylomeDB; P96589; -.
DR BioCyc; BSUB:BSU04320-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR002293; AA/rel_permease1.
DR Pfam; PF13520; AA_permease_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Ion transport; Membrane; Potassium;
KW Potassium transport; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..607
FT /note="Potassium transporter KimA"
FT /id="PRO_0000376842"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 254..274
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 299..319
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 346..366
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 373..393
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 408..428
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 430..450
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 607 AA; 66771 MW; A307AEB08E72B372 CRC64;
MYHSIKRFLI GKPLKSQAAG EQKLTKLKAL AMLSSDALSS VAYGTEQILI ILATISAAAF
WYSIPIAVGV LILLLALILS YRQIIYAYPQ GGGAYIVSKE NLGEKPGLIA GGSLLVDYIL
TVAVSISAGT DAITSAFPAL HDYHVPIAIF LVLVIMILNL RGLSESASIL AYPVYLFVVA
LLVLIAVGLF KLMTGQIDQP AHHTSLGTPV AGITLFLLLK AFSSGCSALT GVEAISNAIP
AFKNPPARNA ARTLAMMGIL LAILFSGITV LAYGYGTAPK PDETVVSQIA SETFGRNVFY
YVIQGVTSLI LVLAANTGFS AFPQLAFNLA RDQYMPRMFT VRGDRLGFSN GIIFLGFASI
VLIILFGGQT EHLIPLYAVG VFIPFTLSQT GMCMKWIKQK PKGWIGKMLI NSCGALISFM
VLSILFVTKF NVVWPVLIFM PIVVLLFFAI KNHYTAVGEQ LRIVDKEPEE IKGTVVIVPV
AGVTTVVQKS IHYAKSLSDQ VIAVHVSFDR EQEKKFEKRW EELNNGVRLV TLHSSYRSLV
HPFDKFLETV EAKAKKEQFS VMVLFPQFIT KKRWHTILHN QSAFLLRVRL FWKKDIMVAT
LPYHFKK
