KINGL_ARATH
ID KINGL_ARATH Reviewed; 447 AA.
AC Q9CAR3; A8MQK6;
DT 27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 119.
DE RecName: Full=SNF1-related protein kinase regulatory subunit gamma-1-like;
DE AltName: Full=AKIN subunit gamma-1-like;
DE AltName: Full=CBS domain-containing protein CBSCBS2;
GN Name=CBSCBS2; OrderedLocusNames=At1g69800; ORFNames=T17F3.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA de los Reyes C., Quan R., Chen H., Bautista V., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19400948; DOI=10.1186/1471-2164-10-200;
RA Kushwaha H.R., Singh A.K., Sopory S.K., Singla-Pareek S.L., Pareek A.;
RT "Genome wide expression analysis of CBS domain containing proteins in
RT Arabidopsis thaliana (L.) Heynh and Oryza sativa L. reveals their
RT developmental and stress regulation.";
RL BMC Genomics 10:200-200(2009).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: Regulatory subunit of the probable trimeric SNF1-related
CC protein kinase (SnRK) complex, which may play a role in a signal
CC transduction cascade regulating gene expression and carbohydrate
CC metabolism in higher plants. {ECO:0000250}.
CC -!- SUBUNIT: Subunit of a probable heterotrimeric complex consisting of an
CC alpha catalytic (KIN10 or KIN11) subunit, and a beta (KINB) and a gamma
CC (KING or SNF4) non-catalytic regulatory subunits. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9CAR3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9CAR3-2; Sequence=VSP_041655;
CC -!- SIMILARITY: Belongs to the 5'-AMP-activated protein kinase gamma
CC subunit family. {ECO:0000305}.
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DR EMBL; AC010675; AAG52563.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34976.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34977.1; -; Genomic_DNA.
DR EMBL; BT033072; ACE82595.1; -; mRNA.
DR EMBL; AK317462; BAH20128.1; -; mRNA.
DR PIR; B96720; B96720.
DR RefSeq; NP_001077801.1; NM_001084332.1. [Q9CAR3-2]
DR RefSeq; NP_564975.2; NM_105648.4. [Q9CAR3-1]
DR AlphaFoldDB; Q9CAR3; -.
DR SMR; Q9CAR3; -.
DR STRING; 3702.AT1G69800.2; -.
DR iPTMnet; Q9CAR3; -.
DR PaxDb; Q9CAR3; -.
DR PRIDE; Q9CAR3; -.
DR ProteomicsDB; 238222; -. [Q9CAR3-1]
DR EnsemblPlants; AT1G69800.1; AT1G69800.1; AT1G69800. [Q9CAR3-1]
DR EnsemblPlants; AT1G69800.2; AT1G69800.2; AT1G69800. [Q9CAR3-2]
DR GeneID; 843316; -.
DR Gramene; AT1G69800.1; AT1G69800.1; AT1G69800. [Q9CAR3-1]
DR Gramene; AT1G69800.2; AT1G69800.2; AT1G69800. [Q9CAR3-2]
DR KEGG; ath:AT1G69800; -.
DR Araport; AT1G69800; -.
DR TAIR; locus:2196789; AT1G69800.
DR eggNOG; KOG1764; Eukaryota.
DR HOGENOM; CLU_036145_0_0_1; -.
DR InParanoid; Q9CAR3; -.
DR OMA; YRWAPFL; -.
DR OrthoDB; 1471847at2759; -.
DR PhylomeDB; Q9CAR3; -.
DR PRO; PR:Q9CAR3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9CAR3; baseline and differential.
DR Genevisible; Q9CAR3; AT.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.10.580.10; -; 2.
DR InterPro; IPR000644; CBS_dom.
DR InterPro; IPR046342; CBS_dom_sf.
DR Pfam; PF00571; CBS; 2.
DR SMART; SM00116; CBS; 4.
DR SUPFAM; SSF54631; SSF54631; 2.
DR PROSITE; PS51371; CBS; 4.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Carbohydrate metabolism; CBS domain;
KW Phosphoprotein; Reference proteome; Repeat.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 2..447
FT /note="SNF1-related protein kinase regulatory subunit
FT gamma-1-like"
FT /id="PRO_0000412194"
FT DOMAIN 54..120
FT /note="CBS 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT DOMAIN 214..275
FT /note="CBS 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT DOMAIN 292..350
FT /note="CBS 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT DOMAIN 374..433
FT /note="CBS 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22223895"
FT MOD_RES 35
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8LBB2"
FT VAR_SEQ 1
FT /note="M -> MHFIRTRVTARKAKPKICLKFFKLSRYQDM (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_041655"
SQ SEQUENCE 447 AA; 47818 MW; 1E8A7EF2204D102A CRC64;
MAGSEAVEDK EIKSAVSSCE AYFEKVQSRK NLPKSLQETL NSAFAGIPVS SFPQVPGGRV
IEIQAETPVS EAVKILSDSK ILSAPVINTD HESSLDWRER YLGIIDYSSI ILWVLESAEL
AAIALSATSA TAAGVGAGAV GALGVAALGM TGPVAAAGLA AAAVGAAVAG GVAAERGIGK
DAPTAADKLG KDFYEVILQE EPFKSTTVRT ILKSFRWAPF LPVSTESSML SVMLLLSKYR
LRNVPVIKTG EPDIKNYVTQ SAVVHGLEGC KGRDWFDHIS ALPISDLGLP FMSPNEVISI
ESEELILEAF KRMRDNNIGG LPVVEGLNKK IVGNISMRDI RYLLLQPEVF SNFRQLTVKS
FATKIATAGE EYGLAIPAIT CRPDSTLGSV INSLASRSVH RVYVAAGDEN ELYGVITLRD
VISCFVSEPP NYFENCLGFS VKEMLNR
