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KIRR2_MOUSE
ID   KIRR2_MOUSE             Reviewed;         700 AA.
AC   Q7TSU7; Q7TQ98;
DT   25-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 140.
DE   RecName: Full=Kin of IRRE-like protein 2;
DE   AltName: Full=Kin of irregular chiasm-like protein 2;
DE   Flags: Precursor;
GN   Name=Kirrel2; Synonyms=Neph3;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Olfactory epithelium;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 48-376 (ISOFORM 2), AND TISSUE SPECIFICITY.
RX   PubMed=12837264; DOI=10.1016/s0888-7543(03)00110-1;
RA   Sun C., Kilburn D., Lukashin A., Crowell T., Gardner H., Brundiers R.,
RA   Diefenbach B., Carulli J.P.;
RT   "Kirrel2, a novel immunoglobulin superfamily gene expressed primarily in
RT   beta cells of the pancreatic islets.";
RL   Genomics 82:130-142(2003).
RN   [3]
RP   INTERACTION WITH NPHS2, AND TISSUE SPECIFICITY.
RC   STRAIN=Swiss Webster; TISSUE=Brain;
RX   PubMed=12424224; DOI=10.1096/fj.02-0242fje;
RA   Sellin L., Huber T.B., Gerke P., Quack I., Pavenstaedt H., Walz G.;
RT   "NEPH1 defines a novel family of podocin interacting proteins.";
RL   FASEB J. 17:115-117(2003).
RN   [4]
RP   INTERACTION WITH NPHS1, AND TISSUE SPECIFICITY.
RX   PubMed=19887377; DOI=10.1074/jbc.m109.060657;
RA   Nishida K., Hoshino M., Kawaguchi Y., Murakami F.;
RT   "Ptf1a directly controls expression of immunoglobulin superfamily molecules
RT   Nephrin and Neph3 in the developing central nervous system.";
RL   J. Biol. Chem. 285:373-380(2010).
RN   [5]
RP   SUBCELLULAR LOCATION, GLYCOSYLATION, INTERACTION WITH FYN, PHOSPHORYLATION
RP   AT SER-563; TYR-595; TYR-596 AND TYR-653, SUBUNIT, PROTEOLYTIC PROCESSING,
RP   DISRUPTION PHENOTYPE, IDENTIFICATION BY MASS SPECTROMETRY, AND MUTAGENESIS
RP   OF 595-TYR-TYR-596; 631-TYR-TYR-632 AND TYR-653.
RX   PubMed=26324709; DOI=10.1074/jbc.m115.684704;
RA   Yesildag B., Bock T., Herrmanns K., Wollscheid B., Stoffel M.;
RT   "Kin of IRRE-like protein 2 is a phosphorylated glycoprotein that regulates
RT   basal insulin secretion.";
RL   J. Biol. Chem. 290:25891-25906(2015).
CC   -!- FUNCTION: May regulate basal insulin secretion.
CC       {ECO:0000269|PubMed:26324709}.
CC   -!- SUBUNIT: Homodimer (PubMed:26324709). Interacts with NPHS2/podocin (via
CC       the C-terminus). Interacts with NPHS1 (via the Ig-like domains).
CC       Interacts with FYN (PubMed:26324709). {ECO:0000269|PubMed:12424224,
CC       ECO:0000269|PubMed:19887377, ECO:0000269|PubMed:26324709}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:26324709};
CC       Single-pass type I membrane protein {ECO:0000305}. Note=Localized along
CC       the sites of the cell contacts (PubMed:26324709). Colocalizes with E-
CC       Cadherin and beta-catenin (PubMed:26324709).
CC       {ECO:0000269|PubMed:26324709}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q7TSU7-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q7TSU7-2; Sequence=VSP_011786;
CC   -!- TISSUE SPECIFICITY: Highly expressed in beta-cells of the pancreatic
CC       islets. Expression is seen in podocytes of kidney glomeruli, and in the
CC       cerebellum and hindbrain at 12.5 dpc, in the spinal cord at 10.5 dpc,
CC       and in retina and hypothalamus at 13.5 dpc.
CC       {ECO:0000269|PubMed:12424224, ECO:0000269|PubMed:12837264,
CC       ECO:0000269|PubMed:19887377}.
CC   -!- PTM: N-glycosylated. {ECO:0000269|PubMed:26324709}.
CC   -!- PTM: Phosphorylated at Ser-548 or Ser-549; due to site ambiguity, the
CC       exact position of the serine phosphorylation could not be determined.
CC       Phosphorylation at residues Tyr-631 and/or Tyr-632. FYN mediates
CC       tyrosine phosphorylation in pancreatic beta-cells.
CC       {ECO:0000269|PubMed:26324709}.
CC   -!- PTM: The extracellular domain is cleaved leading to the generation of a
CC       soluble fragment and a membrane-bound C-terminal fragment, which is
CC       further cleaved by gamma-secretase. {ECO:0000269|PubMed:26324709}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype. However basal insulin
CC       secretion is modestly increased in pancreatic islets of KIRREL2
CC       deficient mice. {ECO:0000269|PubMed:26324709}.
CC   -!- SIMILARITY: Belongs to the immunoglobulin superfamily. {ECO:0000305}.
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DR   EMBL; BC052773; AAH52773.1; -; mRNA.
DR   EMBL; AY305303; AAP72168.1; -; mRNA.
DR   CCDS; CCDS39883.1; -. [Q7TSU7-1]
DR   RefSeq; NP_766486.1; NM_172898.3. [Q7TSU7-1]
DR   PDB; 7LTW; X-ray; 1.80 A; A/B=22-119.
DR   PDBsum; 7LTW; -.
DR   AlphaFoldDB; Q7TSU7; -.
DR   SASBDB; Q7TSU7; -.
DR   SMR; Q7TSU7; -.
DR   STRING; 10090.ENSMUSP00000039395; -.
DR   GlyGen; Q7TSU7; 3 sites.
DR   iPTMnet; Q7TSU7; -.
DR   PhosphoSitePlus; Q7TSU7; -.
DR   PaxDb; Q7TSU7; -.
DR   PRIDE; Q7TSU7; -.
DR   ProteomicsDB; 264995; -. [Q7TSU7-1]
DR   ProteomicsDB; 264996; -. [Q7TSU7-2]
DR   Antibodypedia; 29564; 280 antibodies from 30 providers.
DR   DNASU; 243911; -.
DR   Ensembl; ENSMUST00000045817; ENSMUSP00000039395; ENSMUSG00000036915. [Q7TSU7-1]
DR   GeneID; 243911; -.
DR   KEGG; mmu:243911; -.
DR   UCSC; uc009gel.1; mouse. [Q7TSU7-1]
DR   UCSC; uc012fho.1; mouse. [Q7TSU7-2]
DR   CTD; 84063; -.
DR   MGI; MGI:2442334; Kirrel2.
DR   VEuPathDB; HostDB:ENSMUSG00000036915; -.
DR   eggNOG; KOG3510; Eukaryota.
DR   GeneTree; ENSGT00940000160603; -.
DR   HOGENOM; CLU_013520_1_1_1; -.
DR   InParanoid; Q7TSU7; -.
DR   OMA; ITWTRRG; -.
DR   PhylomeDB; Q7TSU7; -.
DR   TreeFam; TF327139; -.
DR   Reactome; R-MMU-373753; Nephrin family interactions.
DR   BioGRID-ORCS; 243911; 0 hits in 72 CRISPR screens.
DR   PRO; PR:Q7TSU7; -.
DR   Proteomes; UP000000589; Chromosome 7.
DR   RNAct; Q7TSU7; protein.
DR   Bgee; ENSMUSG00000036915; Expressed in spinal cord ventricular layer and 43 other tissues.
DR   ExpressionAtlas; Q7TSU7; baseline and differential.
DR   GO; GO:0005911; C:cell-cell junction; IDA:UniProtKB.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR   GO; GO:0036057; C:slit diaphragm; IDA:MGI.
DR   GO; GO:0050839; F:cell adhesion molecule binding; IBA:GO_Central.
DR   GO; GO:0042802; F:identical protein binding; IDA:UniProtKB.
DR   GO; GO:0098609; P:cell-cell adhesion; IGI:MGI.
DR   GO; GO:0001933; P:negative regulation of protein phosphorylation; IGI:MGI.
DR   Gene3D; 2.60.40.10; -; 5.
DR   InterPro; IPR013162; CD80_C2-set.
DR   InterPro; IPR007110; Ig-like_dom.
DR   InterPro; IPR036179; Ig-like_dom_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR013098; Ig_I-set.
DR   InterPro; IPR003599; Ig_sub.
DR   InterPro; IPR003598; Ig_sub2.
DR   Pfam; PF08205; C2-set_2; 1.
DR   Pfam; PF07679; I-set; 1.
DR   SMART; SM00409; IG; 5.
DR   SMART; SM00408; IGc2; 3.
DR   SUPFAM; SSF48726; SSF48726; 5.
DR   PROSITE; PS50835; IG_LIKE; 4.
PE   1: Evidence at protein level;
KW   3D-structure; Alternative splicing; Cell membrane; Disulfide bond;
KW   Glycoprotein; Immunoglobulin domain; Membrane; Phosphoprotein;
KW   Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..700
FT                   /note="Kin of IRRE-like protein 2"
FT                   /id="PRO_0000015097"
FT   TOPO_DOM        20..507
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        508..528
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        529..700
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          21..115
FT                   /note="Ig-like C2-type 1"
FT   DOMAIN          120..219
FT                   /note="Ig-like C2-type 2"
FT   DOMAIN          224..304
FT                   /note="Ig-like C2-type 3"
FT   DOMAIN          309..391
FT                   /note="Ig-like C2-type 4"
FT   DOMAIN          395..497
FT                   /note="Ig-like C2-type 5"
FT   REGION          542..576
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          671..700
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           146..148
FT                   /note="Cell attachment site"
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        542..558
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        559..576
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         563
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:26324709"
FT   MOD_RES         595
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000269|PubMed:26324709"
FT   MOD_RES         596
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000269|PubMed:26324709"
FT   MOD_RES         653
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000269|PubMed:26324709"
FT   CARBOHYD        140
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        298
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        481
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        42..100
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT   DISULFID        143..201
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT   DISULFID        245..288
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT   DISULFID        330..372
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT   DISULFID        416..482
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT   VAR_SEQ         118..136
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:12837264"
FT                   /id="VSP_011786"
FT   MUTAGEN         595..596
FT                   /note="YY->FF: Enhances stability. Modifies the structure
FT                   of cell contacts. Localization at the plasma membrane is
FT                   increased. Does not affect dimerization. No change in
FT                   shedding activity."
FT                   /evidence="ECO:0000269|PubMed:26324709"
FT   MUTAGEN         631..632
FT                   /note="YY->FF: Does not modifies the structure of cell
FT                   contacts. No effect on localization at the plasma membrane.
FT                   No change in shedding activity."
FT                   /evidence="ECO:0000269|PubMed:26324709"
FT   MUTAGEN         653
FT                   /note="Y->F: Does not modifies the structure of cell
FT                   contacts. No effect on localization at the plasma membrane.
FT                   No change in shedding activity."
FT                   /evidence="ECO:0000269|PubMed:26324709"
FT   CONFLICT        48
FT                   /note="R -> W (in Ref. 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        166
FT                   /note="G -> A (in Ref. 2; AAP72168)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        376
FT                   /note="P -> A (in Ref. 2; AAP72168)"
FT                   /evidence="ECO:0000305"
FT   STRAND          22..25
FT                   /evidence="ECO:0007829|PDB:7LTW"
FT   STRAND          30..33
FT                   /evidence="ECO:0007829|PDB:7LTW"
FT   STRAND          38..40
FT                   /evidence="ECO:0007829|PDB:7LTW"
FT   STRAND          43..46
FT                   /evidence="ECO:0007829|PDB:7LTW"
FT   STRAND          51..55
FT                   /evidence="ECO:0007829|PDB:7LTW"
FT   STRAND          72..76
FT                   /evidence="ECO:0007829|PDB:7LTW"
FT   TURN            78..81
FT                   /evidence="ECO:0007829|PDB:7LTW"
FT   STRAND          84..89
FT                   /evidence="ECO:0007829|PDB:7LTW"
FT   HELIX           92..94
FT                   /evidence="ECO:0007829|PDB:7LTW"
FT   STRAND          96..102
FT                   /evidence="ECO:0007829|PDB:7LTW"
FT   TURN            103..106
FT                   /evidence="ECO:0007829|PDB:7LTW"
FT   STRAND          112..117
FT                   /evidence="ECO:0007829|PDB:7LTW"
SQ   SEQUENCE   700 AA;  74529 MW;  5D9404C2E13B18B8 CRC64;
     MLASALLVFL CCFKGHAGSS PHFLQQPEDM VVLLGEEARL PCALGAYRGL VQWTKDGLAL
     GGERDLPGWS RYWISGNSAS GQHDLHIKPV ELEDEASYEC QASQAGLRSR PAQLHVMVPP
     EAPQVLGGPS VSLVAGVPGN LTCRSRGDSR PAPELLWFRD GIRLDGSSFH QTTLKDKATG
     TVENTLFLTP SSHDDGATLI CRARSQALPT GRDTAVTLSL QYPPMVTLSA EPQTVQEGEK
     VTFLCQATAQ PPVTGYRWAK GGSPVLGARG PRLEVVADAT FLTEPVSCEV SNAVGSANRS
     TALEVLYGPI LQAKPKSVSV DVGKDASFSC VWRGNPLPRI TWTRMGGSQV LSSGPTLRLP
     SVALEDAGDY VCRAEPRRTG LGGGKAQARL TVNAPPVVTA LQPAPAFLRG PARLQCVVFA
     SPAPDSVVWS WDEGFLEAGS LGRFLVEAFP APEVEGGQGP GLISVLHISG TQESDFTTGF
     NCSARNRLGE GRVQIHLGRR DLLPTVRIVA GAASAATSLL MVITGVVLCC WRHGSLSKQK
     NLVRIPGSSE GSSSRGPEEE TGSSEDRGPI VHTDHSDLVL EEKEALETKD PTNGYYRVRG
     VSVSLSLGEA PGGGLFLPPP SPIGLPGTPT YYDFKPHLDL VPPCRLYRAR AGYLTTPHPR
     AFTSYMKPTS FGPPELSSGT PPFPYATLSP PSHQRLQTHV
 
 
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