KISH_DROME
ID KISH_DROME Reviewed; 72 AA.
AC Q9VWH8;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 19-OCT-2011, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Protein kish;
DE Flags: Precursor;
GN Name=ksh; ORFNames=CG14199;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=19942856; DOI=10.1038/emboj.2009.350;
RA Wendler F., Gillingham A.K., Sinka R., Rosa-Ferreira C., Gordon D.E.,
RA Franch-Marro X., Peden A.A., Vincent J.P., Munro S.;
RT "A genome-wide RNA interference screen identifies two novel components of
RT the metazoan secretory pathway.";
RL EMBO J. 29:304-314(2010).
CC -!- FUNCTION: Involved in the early part of the secretory pathway.
CC {ECO:0000269|PubMed:19942856}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Single-
CC pass type I membrane protein {ECO:0000250}. Endoplasmic reticulum
CC membrane {ECO:0000250}; Single-pass type I membrane protein
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: Kish means 'small' in Hungarian.
CC -!- SIMILARITY: Belongs to the KISH family. {ECO:0000305}.
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DR EMBL; AE014298; AAF48961.2; -; Genomic_DNA.
DR RefSeq; NP_652499.2; NM_144242.4.
DR AlphaFoldDB; Q9VWH8; -.
DR BioGRID; 72652; 2.
DR IntAct; Q9VWH8; 9.
DR STRING; 7227.FBpp0289451; -.
DR GlyGen; Q9VWH8; 1 site.
DR PaxDb; Q9VWH8; -.
DR PRIDE; Q9VWH8; -.
DR DNASU; 50363; -.
DR EnsemblMetazoa; FBtr0300214; FBpp0289451; FBgn0040890.
DR GeneID; 50363; -.
DR KEGG; dme:Dmel_CG14199; -.
DR UCSC; CG14199-RB; d. melanogaster.
DR CTD; 50363; -.
DR FlyBase; FBgn0040890; ksh.
DR VEuPathDB; VectorBase:FBgn0040890; -.
DR eggNOG; KOG3808; Eukaryota.
DR GeneTree; ENSGT00940000155186; -.
DR HOGENOM; CLU_152663_1_1_1; -.
DR InParanoid; Q9VWH8; -.
DR OMA; ICSCTYV; -.
DR OrthoDB; 1596132at2759; -.
DR PhylomeDB; Q9VWH8; -.
DR BioGRID-ORCS; 50363; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 50363; -.
DR PRO; PR:Q9VWH8; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0040890; Expressed in brain and 11 other tissues.
DR ExpressionAtlas; Q9VWH8; baseline and differential.
DR Genevisible; Q9VWH8; DM.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:FlyBase.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; IDA:FlyBase.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0046907; P:intracellular transport; IMP:FlyBase.
DR GO; GO:0009306; P:protein secretion; IMP:FlyBase.
DR InterPro; IPR009653; Ksh1.
DR Pfam; PF06842; DUF1242; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; Glycoprotein; Golgi apparatus; Membrane;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..72
FT /note="Protein kish"
FT /id="PRO_0000247774"
FT TOPO_DOM 27..53
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..71
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 72
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 35
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 72 AA; 8176 MW; F8A584317A07A73D CRC64;
MSALFNFHSL LSVILLLICT CAYLRSLFPS LIDRNKTGFM GTFWKLARIG ERKSPWVGAA
CLIMAFTVLF WS
