KISH_YEAST
ID KISH_YEAST Reviewed; 72 AA.
AC Q8TGJ3; D6W1F4;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Protein Kish {ECO:0000303|PubMed:19942856};
DE Flags: Precursor;
GN Name=KSH1 {ECO:0000303|PubMed:19942856};
GN OrderedLocusNames=YNL024C-A {ECO:0000312|SGD:S000028698};
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11753363; DOI=10.1038/nbt0102-58;
RA Kumar A., Harrison P.M., Cheung K.-H., Lan N., Echols N., Bertone P.,
RA Miller P., Gerstein M.B., Snyder M.;
RT "An integrated approach for finding overlooked genes in yeast.";
RL Nat. Biotechnol. 20:58-63(2002).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=19942856; DOI=10.1038/emboj.2009.350;
RA Wendler F., Gillingham A.K., Sinka R., Rosa-Ferreira C., Gordon D.E.,
RA Franch-Marro X., Peden A.A., Vincent J.P., Munro S.;
RT "A genome-wide RNA interference screen identifies two novel components of
RT the metazoan secretory pathway.";
RL EMBO J. 29:304-314(2010).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=26928762; DOI=10.1038/nmeth.3795;
RA Yofe I., Weill U., Meurer M., Chuartzman S., Zalckvar E., Goldman O.,
RA Ben-Dor S., Schuetze C., Wiedemann N., Knop M., Khmelinskii A.,
RA Schuldiner M.;
RT "One library to make them all: streamlining the creation of yeast libraries
RT via a SWAp-Tag strategy.";
RL Nat. Methods 13:371-378(2016).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=29897761; DOI=10.1021/acs.jproteome.8b00032;
RA He C., Jia C., Zhang Y., Xu P.;
RT "Enrichment-based proteogenomics identifies microproteins, missing
RT proteins, and novel smORFs in Saccharomyces cerevisiae.";
RL J. Proteome Res. 17:2335-2344(2018).
CC -!- FUNCTION: Involved in the early part of the secretory pathway.
CC {ECO:0000269|PubMed:19942856}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:26928762}; Single-pass type I membrane protein
CC {ECO:0000255}. Golgi apparatus membrane {ECO:0000269|PubMed:19942856};
CC Single-pass type I membrane protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Inviable. {ECO:0000269|PubMed:19942856}.
CC -!- MISCELLANEOUS: 'Kish' means small in Hungarian.
CC {ECO:0000305|PubMed:19942856}.
CC -!- SIMILARITY: Belongs to the KISH family. {ECO:0000305}.
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DR EMBL; Z71300; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; Z71301; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AF480014; AAL79327.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10520.1; -; Genomic_DNA.
DR RefSeq; NP_878158.3; NM_001184619.3.
DR AlphaFoldDB; Q8TGJ3; -.
DR BioGRID; 37058; 5.
DR STRING; 4932.YNL024C-A; -.
DR MaxQB; Q8TGJ3; -.
DR PaxDb; Q8TGJ3; -.
DR PRIDE; Q8TGJ3; -.
DR EnsemblFungi; YNL024C-A_mRNA; YNL024C-A; YNL024C-A.
DR GeneID; 1466516; -.
DR KEGG; sce:YNL024C-A; -.
DR SGD; S000028698; KSH1.
DR VEuPathDB; FungiDB:YNL024C-A; -.
DR eggNOG; KOG3808; Eukaryota.
DR GeneTree; ENSGT00940000155186; -.
DR HOGENOM; CLU_152663_1_0_1; -.
DR InParanoid; Q8TGJ3; -.
DR OMA; VGCFMMG; -.
DR BioCyc; YEAST:G3O-33410-MON; -.
DR PRO; PR:Q8TGJ3; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; Q8TGJ3; protein.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0046907; P:intracellular transport; IBA:GO_Central.
DR GO; GO:0009306; P:protein secretion; IBA:GO_Central.
DR InterPro; IPR009653; Ksh1.
DR Pfam; PF06842; DUF1242; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Golgi apparatus; Membrane; Reference proteome;
KW Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..72
FT /note="Protein Kish"
FT /id="PRO_0000247775"
FT TOPO_DOM 27..53
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..72
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 72 AA; 8196 MW; 5645433999B16B27 CRC64;
MSALFNFRSL LQVILLLICS CSYVHGQWPS LLDRYKNHEV LGAFWKMARV GERASPYVSL
ACILMAISQF NS
