KISS1_RAT
ID KISS1_RAT Reviewed; 130 AA.
AC Q7TSB7;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Metastasis-suppressor KiSS-1;
DE AltName: Full=Kisspeptin-1;
DE Contains:
DE RecName: Full=Metastin;
DE Contains:
DE RecName: Full=Kisspeptin-10;
DE AltName: Full=Metastin45-54;
DE Flags: Precursor;
GN Name=Kiss1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=Wistar; TISSUE=Liver, and Placenta;
RX PubMed=15157736; DOI=10.1016/j.bbaexp.2004.02.005;
RA Terao Y., Kumano S., Takatsu Y., Hattori M., Nishimura A., Ohtaki T.,
RA Shintani Y.;
RT "Expression of KiSS-1, a metastasis suppressor gene, in trophoblast giant
RT cells of the rat placenta.";
RL Biochim. Biophys. Acta 1678:102-110(2004).
RN [2]
RP POSSIBLE ROLE IN GONADOPTROPIN RELEASE.
RX PubMed=15219839; DOI=10.1016/j.bbrc.2004.05.185;
RA Matsui H., Takatsu Y., Kumano S., Matsumoto H., Ohtaki T.;
RT "Peripheral administration of metastin induces marked gonadotropin release
RT and ovulation in the rat.";
RL Biochem. Biophys. Res. Commun. 320:383-388(2004).
RN [3]
RP TISSUE SPECIFICITY, AND FUNCTION.
RX PubMed=15242985; DOI=10.1210/en.2004-0413;
RA Navarro V.M., Castellano J.M., Fernandez-Fernandez R., Barreiro M.L.,
RA Roa J., Sanchez-Criado J.E., Aguilar E., Dieguez C., Pinilla L.,
RA Tena-Sempere M.;
RT "Developmental and hormonally regulated messenger ribonucleic acid
RT expression of KiSS-1 and its putative receptor, GPR54, in rat hypothalamus
RT and potent luteinizing hormone-releasing activity of KiSS-1 peptide.";
RL Endocrinology 145:4565-4574(2004).
RN [4]
RP TISSUE SPECIFICITY, AND FUNCTION.
RX PubMed=15593369; DOI=10.1002/cne.20350;
RA Brailoiu G.C., Dun S.L., Ohsawa M., Yin D., Yang J., Chang J.K.,
RA Brailoiu E., Dun N.J.;
RT "KiSS-1 expression and metastin-like immunoreactivity in the rat brain.";
RL J. Comp. Neurol. 481:314-329(2005).
CC -!- FUNCTION: Metastasis suppressor protein. May regulate events downstream
CC of cell-matrix adhesion, perhaps involving cytoskeletal reorganization.
CC Generates a C-terminally amidated peptide, metastin which functions as
CC the endogenous ligand of the G-protein coupled receptor GPR54. The
CC receptor is also essential for normal gonadotropin-released hormone
CC physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a
CC pivotal factor in central regulation of the gonadotropic axis at
CC puberty and in adulthood. Intracerebroventricular administration
CC induces an increase in serum LH and FSH levels in prepubertal male and
CC female as well as in adult animals. {ECO:0000269|PubMed:15242985,
CC ECO:0000269|PubMed:15593369}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Highest levels in the cecum and colon. Moderate
CC levels present in the liver, spleen, kidney, ovary, uterus and small
CC intestine. Low levels in the stomach, pancreas and placenta. Expressed
CC only moderately in the placenta. Persistent expression is detected in
CC hypothalamus throughout postnatal development, with maximum expression
CC levels at puberty in both male and female. Hypothalamic expression is
CC sensitive to neonatal imprinting by estrogen. Expression is higher in
CC the hypothalamus than in the brainstem and spinal cord. In the brain,
CC metastin-like immunoreactivity is found mainly in three groups of
CC cells: dorsomedial hypothalamic nucleus, nucleus of the solitary tract,
CC and caudal ventrolateral medulla. {ECO:0000269|PubMed:15157736,
CC ECO:0000269|PubMed:15242985, ECO:0000269|PubMed:15593369}.
CC -!- DEVELOPMENTAL STAGE: Expression observed in trophoblast giant cells
CC (TGCs), the placenta-derived cell lineage aligned at the boundary
CC between the uterus and placenta, at embryonic days 12.5 (E12.5).
CC Expressions gradually decreased during placental maturation, and the
CC signal is no more detectable in the cells at E18.5.
CC {ECO:0000269|PubMed:15157736}.
CC -!- SIMILARITY: Belongs to the KISS1 family. {ECO:0000305}.
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DR EMBL; AY196983; AAP22375.1; -; mRNA.
DR RefSeq; NP_859043.1; NM_181692.1.
DR RefSeq; XP_008767665.1; XM_008769443.2.
DR RefSeq; XP_017454186.1; XM_017598697.1.
DR AlphaFoldDB; Q7TSB7; -.
DR BioGRID; 252772; 1.
DR STRING; 10116.ENSRNOP00000068332; -.
DR PhosphoSitePlus; Q7TSB7; -.
DR PaxDb; Q7TSB7; -.
DR Ensembl; ENSRNOT00000076506; ENSRNOP00000068332; ENSRNOG00000047481.
DR GeneID; 289023; -.
DR KEGG; rno:289023; -.
DR UCSC; RGD:727850; rat.
DR CTD; 3814; -.
DR RGD; 727850; Kiss1.
DR eggNOG; ENOG502SFM2; Eukaryota.
DR GeneTree; ENSGT00390000008827; -.
DR HOGENOM; CLU_119112_0_0_1; -.
DR InParanoid; Q7TSB7; -.
DR OMA; PMENPRS; -.
DR OrthoDB; 1513721at2759; -.
DR Reactome; R-RNO-375276; Peptide ligand-binding receptors.
DR Reactome; R-RNO-416476; G alpha (q) signalling events.
DR PRO; PR:Q7TSB7; -.
DR Proteomes; UP000002494; Chromosome 13.
DR Bgee; ENSRNOG00000047481; Expressed in pancreas and 11 other tissues.
DR ExpressionAtlas; Q7TSB7; baseline and differential.
DR GO; GO:0016324; C:apical plasma membrane; IDA:RGD.
DR GO; GO:0005737; C:cytoplasm; IDA:RGD.
DR GO; GO:0005615; C:extracellular space; IDA:RGD.
DR GO; GO:0043005; C:neuron projection; IDA:RGD.
DR GO; GO:0043025; C:neuronal cell body; IDA:RGD.
DR GO; GO:0001664; F:G protein-coupled receptor binding; TAS:RGD.
DR GO; GO:0031773; F:kisspeptin receptor binding; IPI:RGD.
DR GO; GO:0046697; P:decidualization; ISO:RGD.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISO:RGD.
DR GO; GO:0060112; P:generation of ovulation cycle rhythm; IMP:RGD.
DR GO; GO:0030336; P:negative regulation of cell migration; ISO:RGD.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IDA:RGD.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IDA:RGD.
DR GO; GO:0051482; P:positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway; IDA:RGD.
DR GO; GO:0060124; P:positive regulation of growth hormone secretion; IDA:RGD.
DR GO; GO:0033686; P:positive regulation of luteinizing hormone secretion; IDA:RGD.
DR GO; GO:0043410; P:positive regulation of MAPK cascade; IMP:RGD.
DR GO; GO:0050806; P:positive regulation of synaptic transmission; IDA:RGD.
DR InterPro; IPR020207; Metastasis-suppressor_KiSS-1.
DR PANTHER; PTHR16955; PTHR16955; 1.
DR Pfam; PF15152; Kisspeptin; 1.
PE 2: Evidence at transcript level;
KW Amidation; Cleavage on pair of basic residues; Disulfide bond;
KW Phosphoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..130
FT /note="Metastasis-suppressor KiSS-1"
FT /id="PRO_0000021555"
FT PEPTIDE 68..119
FT /note="Metastin"
FT /id="PRO_0000021556"
FT PEPTIDE 110..119
FT /note="Kisspeptin-10"
FT /id="PRO_0000021557"
FT REGION 52..91
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 110..119
FT /note="Essential for receptor binding and receptor
FT activation"
FT MOD_RES 110
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q15726"
FT MOD_RES 119
FT /note="Tyrosine amide"
FT /evidence="ECO:0000250"
FT DISULFID 71..85
FT /evidence="ECO:0000255"
SQ SEQUENCE 130 AA; 14188 MW; 9ADEADA018BF5868 CRC64;
MISLASWQLL LLLCVASFGE PLAKMAPVVN PEPTGQQSGP QELVNAWQKG PRYAESKPGA
AGLRARRTSP CPPVENPTGH QRPPCATRSR LIPAPRGSVL VQREKDMSAY NWNSFGLRYG
RRQVARAARG
