KISSR_MOUSE
ID KISSR_MOUSE Reviewed; 396 AA.
AC Q91V45; Q8K3P2;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=KiSS-1 receptor;
DE Short=KiSS-1R;
DE AltName: Full=G-protein coupled receptor 54;
DE AltName: Full=G-protein coupled receptor OT7T175;
DE Short=mOT7T175;
DE AltName: Full=Kisspeptins receptor;
DE AltName: Full=Metastin receptor;
GN Name=Kiss1r; Synonyms=Gpr54;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=11414709; DOI=10.1006/bbrc.2001.5098;
RA Clements M.K., McDonald T.P., Wang R., Xie G., O'Dowd B.F., George S.R.,
RA Austin C.P., Liu Q.;
RT "FMRFamide-related neuropeptides are agonists of the orphan G-protein-
RT coupled receptor GPR54.";
RL Biochem. Biophys. Res. Commun. 284:1189-1193(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=12359743;
RA Stafford L.J., Xia C., Ma W., Cai Y., Liu M.;
RT "Identification and characterization of mouse metastasis-suppressor KiSS1
RT and its G-protein-coupled receptor.";
RL Cancer Res. 62:5399-5404(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Spinal cord;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP REVIEW.
RX PubMed=15519892; DOI=10.1016/j.tem.2004.09.008;
RA Colledge W.H.;
RT "GPR54 and puberty.";
RL Trends Endocrinol. Metab. 15:448-453(2004).
RN [6]
RP INVOLVEMENT IN SEXUAL DEVELOPMENT.
RX PubMed=14652023; DOI=10.1016/j.bbrc.2003.11.066;
RA Funes S., Hedrick J.A., Vassileva G., Markowitz L., Abbondanzo S.,
RA Golovko A., Yang S., Monsma F.J., Gustafson E.L.;
RT "The KiSS-1 receptor GPR54 is essential for the development of the murine
RT reproductive system.";
RL Biochem. Biophys. Res. Commun. 312:1357-1363(2003).
RN [7]
RP INVOLVEMENT IN SEXUAL DEVELOPMENT.
RX PubMed=14573733; DOI=10.1056/nejmoa035322;
RA Seminara S.B., Messager S., Chatzidaki E.E., Thresher R.R.,
RA Acierno J.S. Jr., Shagoury J.K., Bo-Abbas Y., Kuohung W., Schwinof K.M.,
RA Hendrick A.G., Zahn D., Dixon J., Kaiser U.B., Slaugenhaupt S.A.,
RA Gusella J.F., O'Rahilly S., Carlton M.B.L., Crowley W.F. Jr.,
RA Aparicio S.A.J.R., Colledge W.H.;
RT "The GPR54 gene as a regulator of puberty.";
RL N. Engl. J. Med. 349:1614-1627(2003).
RN [8]
RP TISSUE SPECIFICITY, AND DIRECT STIMULATION OF GONADOTROPIN-RELEASING
RP HORMONE RELEASE.
RX PubMed=15665093; DOI=10.1073/pnas.0409330102;
RA Messager S., Chatzidaki E.E., Ma D., Hendrick A.G., Zahn D., Dixon J.,
RA Thresher R.R., Malinge I., Lomet D., Carlton M.B., Colledge W.H.,
RA Caraty A., Aparicio S.A.J.R.;
RT "Kisspeptin directly stimulates gonadotropin-releasing hormone release via
RT G protein-coupled receptor 54.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:1761-1766(2005).
CC -!- FUNCTION: Receptor for metastin (kisspeptin-52 or kp-52), a C-
CC terminally amidated peptide of KiSS1. KiSS1 is a metastasis suppressor
CC protein. Activation of the receptor inhibits cell proliferation and
CC cell migration, key characteristics of tumor metastasis. The receptor
CC is essential for normal gonadotropin-released hormone physiology and
CC for puberty. The hypothalamic KiSS1/KISS1R system is a pivotal factor
CC in central regulation of the gonadotropic axis at puberty and in
CC adulthood. Analysis of the transduction pathways activated by the
CC receptor identifies coupling to phospholipase C and intracellular
CC calcium release through pertussis toxin-insensitive G(q) proteins.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Highest level in the heart and 15- and 17-day
CC embryos. Low level in other tissues. Colocalized with gonadotropin-
CC releasing hormone (GnRH) neurons in the hypothalamus.
CC {ECO:0000269|PubMed:11414709, ECO:0000269|PubMed:12359743,
CC ECO:0000269|PubMed:15665093}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AF343726; AAK83236.1; -; mRNA.
DR EMBL; AY044228; AAK95814.1; -; mRNA.
DR EMBL; AK039628; BAC30404.1; -; mRNA.
DR EMBL; BC016531; AAH16531.1; -; mRNA.
DR CCDS; CCDS23998.1; -.
DR RefSeq; NP_444474.1; NM_053244.5.
DR RefSeq; XP_006513139.1; XM_006513076.3.
DR RefSeq; XP_006513140.1; XM_006513077.3.
DR AlphaFoldDB; Q91V45; -.
DR SMR; Q91V45; -.
DR STRING; 10090.ENSMUSP00000040516; -.
DR GuidetoPHARMACOLOGY; 266; -.
DR GlyGen; Q91V45; 3 sites.
DR iPTMnet; Q91V45; -.
DR PhosphoSitePlus; Q91V45; -.
DR PaxDb; Q91V45; -.
DR PRIDE; Q91V45; -.
DR Antibodypedia; 10260; 355 antibodies from 34 providers.
DR DNASU; 114229; -.
DR Ensembl; ENSMUST00000045529; ENSMUSP00000040516; ENSMUSG00000035773.
DR GeneID; 114229; -.
DR KEGG; mmu:114229; -.
DR UCSC; uc007gao.2; mouse.
DR CTD; 84634; -.
DR MGI; MGI:2148793; Kiss1r.
DR VEuPathDB; HostDB:ENSMUSG00000035773; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT00940000157017; -.
DR HOGENOM; CLU_009579_6_4_1; -.
DR InParanoid; Q91V45; -.
DR OMA; QTYCSES; -.
DR OrthoDB; 1294084at2759; -.
DR PhylomeDB; Q91V45; -.
DR TreeFam; TF315737; -.
DR Reactome; R-MMU-375276; Peptide ligand-binding receptors.
DR Reactome; R-MMU-416476; G alpha (q) signalling events.
DR BioGRID-ORCS; 114229; 5 hits in 73 CRISPR screens.
DR PRO; PR:Q91V45; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q91V45; protein.
DR Bgee; ENSMUSG00000035773; Expressed in granulocyte and 59 other tissues.
DR ExpressionAtlas; Q91V45; baseline and differential.
DR Genevisible; Q91V45; MM.
DR GO; GO:0009986; C:cell surface; IDA:MGI.
DR GO; GO:0005929; C:cilium; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; ISO:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0016020; C:membrane; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0008528; F:G protein-coupled peptide receptor activity; IDA:MGI.
DR GO; GO:0004930; F:G protein-coupled receptor activity; ISO:MGI.
DR GO; GO:0042923; F:neuropeptide binding; ISO:MGI.
DR GO; GO:0008188; F:neuropeptide receptor activity; ISO:MGI.
DR GO; GO:0050482; P:arachidonic acid secretion; ISO:MGI.
DR GO; GO:0019722; P:calcium-mediated signaling; ISO:MGI.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISO:MGI.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISO:MGI.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IBA:GO_Central.
DR GO; GO:0046887; P:positive regulation of hormone secretion; ISO:MGI.
DR GO; GO:0043410; P:positive regulation of MAPK cascade; ISO:MGI.
DR GO; GO:0051496; P:positive regulation of stress fiber assembly; ISO:MGI.
DR GO; GO:0050806; P:positive regulation of synaptic transmission; ISO:MGI.
DR GO; GO:0007165; P:signal transduction; IDA:MGI.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR008103; KiSS_1_rcpt.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR01728; KISS1RECEPTR.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..396
FT /note="KiSS-1 receptor"
FT /id="PRO_0000069696"
FT TOPO_DOM 1..43
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..66
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..78
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..101
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 102..116
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..138
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 139..157
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..180
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 181..203
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 225..260
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..283
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 284..305
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 306..330
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 331..396
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 349..396
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 10
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 18
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 28
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 115..191
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 82
FT /note="Missing (in Ref. 2; AAK95814)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 396 AA; 43061 MW; B5354E739A1F185B CRC64;
MATEATLAPN VTWWAPSNAS GCPGCGVNAS DDPGSAPRPL DAWLVPLFFA TLMLLGLVGN
SLVIYVICRH KHMQTVTNFY IANLAATDVT FLLCCVPFTA LLYPLPAWVL GDFMCKFVNY
IQQVSVQATC ATLTAMSVDR WYVTVFPLRA LHRRTPRLAL AVSLSIWVGS AAVSAPVLAL
HRLSPGPRTY CSEAFPSRAL ERAFALYNLL ALYLLPLLAT CACYGAMLRH LGRAAVRPAP
TDGALQGQLL AQRAGAVRTK VSRLVAAVVL LFAACWGPIQ LFLVLQALGP SGAWHPRSYA
AYAVKIWAHC MSYSNSALNP LLYAFLGSHF RQAFCRVCPC CRQRQRRPHT SAHSDRAATH
TVPHSRAAHP VRIRSPEPGN PVVRSPCAQS ERTASL
