ID   KITH_ASFB7              Reviewed;         196 AA.
AC   P18555;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Thymidine kinase;
DE            Short=TDK;
DE            EC=2.7.1.21;
GN   OrderedLocusNames=Ba71V-050; ORFNames=K196R;
OS   African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V)
OS   (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10498;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND IDENTIFICATION.
RX   PubMed=2389555; DOI=10.1016/0042-6822(90)90409-k;
RA   Blasco R., Lopez-Otin C., Munoz M., Bockamp E.-O., Simon-Mateo C.,
RA   Vinuela E.;
RT   "Sequence and evolutionary relationships of African swine fever virus
RT   thymidine kinase.";
RL   Virology 178:301-304(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1987368; DOI=10.1128/jvi.65.2.1046-1052.1991;
RA   Hernandez A.M., Taberes E.;
RT   "Expression and characterization of the thymidine kinase gene of African
RT   swine fever virus.";
RL   J. Virol. 65:1046-1052(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11831707; DOI=10.1006/viro.1995.1149;
RA   Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C.,
RA   Rodriguez J.F., Vinuela E.;
RT   "Analysis of the complete nucleotide sequence of African swine fever
RT   virus.";
RL   Virology 208:249-278(1995).
CC   -!- FUNCTION: Phosphorylates thymidine. ASFV replicates in the cytoplasm of
CC       infected cells and contains genes encoding a number of enzymes needed
CC       for DNA synthesis, including thymidine kinase. Important for growth in
CC       swine macrophages in vitro and is a virus virulence factor in swine (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + thymidine = ADP + dTMP + H(+); Xref=Rhea:RHEA:19129,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17748, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:63528, ChEBI:CHEBI:456216; EC=2.7.1.21;
CC   -!- SIMILARITY: Belongs to the thymidine kinase family. {ECO:0000305}.
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DR   EMBL; Z21490; CAG33722.1; -; Genomic_DNA.
DR   EMBL; M63119; AAA42737.1; -; Genomic_DNA.
DR   EMBL; U18466; AAA65280.1; -; Genomic_DNA.
DR   PIR; A37079; KIXFAS.
DR   RefSeq; NP_042744.1; NC_001659.2.
DR   SMR; P18555; -.
DR   GeneID; 22220432; -.
DR   KEGG; vg:22220432; -.
DR   Proteomes; UP000000624; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004797; F:thymidine kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071897; P:DNA biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001267; Thymidine_kinase.
DR   InterPro; IPR020633; Thymidine_kinase_CS.
DR   PANTHER; PTHR11441; PTHR11441; 1.
DR   Pfam; PF00265; TK; 1.
DR   PIRSF; PIRSF035805; TK_cell; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00603; TK_CELLULAR_TYPE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA synthesis; Kinase; Metal-binding; Nucleotide-binding;
KW   Reference proteome; Transferase; Virulence; Zinc.
FT   CHAIN           1..196
FT                   /note="Thymidine kinase"
FT                   /id="PRO_0000174927"
FT   ACT_SITE        92
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
FT   BINDING         17..24
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000305"
FT   BINDING         121
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         146
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         149
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         166..170
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         179
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         182
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   196 AA;  22421 MW;  94AA5C64C98985A0 CRC64;
     MNIIRKLKPG TISLVLGPMF AGKTTFLIHC IYMLERLEKK VVFIKSTKNT RDKTIKTHSG
     IQLRPKQCKI IESTQLSDVG SLTDIHAVVV DEAHFFDDLI KCRTWAEEEK IIILAGLNAS
     FEQKMFPPIV RIFPYCSWVK YIGRTCMKCN QHNACFNVRK NADKTLILAG GSELYVTCCN
     NCLKNTFIKQ LQPIKY