KITH_FOWPN
ID KITH_FOWPN Reviewed; 183 AA.
AC P10052;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Thymidine kinase;
DE EC=2.7.1.21;
GN Name=TK; OrderedLocusNames=FPV086;
OS Fowlpox virus (strain NVSL) (FPV).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Avipoxvirus.
OX NCBI_TaxID=928301;
OH NCBI_TaxID=7742; Vertebrata.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3027984; DOI=10.1016/0042-6822(87)90415-6;
RA Boyle D.B., Coupar B.E.H., Gibbs A.J., Seigman L.J., Both G.W.;
RT "Fowlpox virus thymidine kinase: nucleotide sequence and relationships to
RT other thymidine kinases.";
RL Virology 156:355-365(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=FP-9 / Isolate HP-440;
RX PubMed=2838574; DOI=10.1099/0022-1317-69-6-1275;
RA Binns M.M., Tomley F.M., Campbell J., Boursnell M.E.G.;
RT "Comparison of a conserved region in fowlpox virus and vaccinia virus
RT genomes and the translocation of the fowlpox virus thymidine kinase gene.";
RL J. Gen. Virol. 69:1275-1283(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=FP-9 / Isolate HP-440;
RX PubMed=1326827; DOI=10.1016/0168-1702(92)90004-s;
RA Binns M.M., Boursnell M.E.G., Skinner M.A.;
RT "Gene translocations in poxviruses: the fowlpox virus thymidine kinase gene
RT is flanked by 15 bp direct repeats and occupies the locus which in vaccinia
RT virus is occupied by the ribonucleotide reductase large subunit gene.";
RL Virus Res. 24:161-172(1992).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Vaccine;
RA Beisel C.E., Nazerian K.;
RL Submitted (APR-1990) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Senthilvelan A., Purushothaman V., Palaniswami K.;
RT "Sequence of thymidine kinase gene of Indian isolate of fowlpox virus.";
RL Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=10729156; DOI=10.1128/jvi.74.8.3815-3831.2000;
RA Afonso C.L., Tulman E.R., Lu Z., Zsak L., Kutish G.F., Rock D.L.;
RT "The genome of fowlpox virus.";
RL J. Virol. 74:3815-3831(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + thymidine = ADP + dTMP + H(+); Xref=Rhea:RHEA:19129,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17748, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:63528, ChEBI:CHEBI:456216; EC=2.7.1.21;
CC -!- SIMILARITY: Belongs to the thymidine kinase family. {ECO:0000305}.
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DR EMBL; M16617; AAA43822.1; -; Genomic_DNA.
DR EMBL; D00321; BAA00233.1; -; Genomic_DNA.
DR EMBL; AJ223385; CAA11295.1; -; Genomic_DNA.
DR EMBL; X52860; CAA37041.1; -; Genomic_DNA.
DR EMBL; AF198100; AAF44430.1; -; Genomic_DNA.
DR EMBL; AF396867; AAK77606.1; -; Genomic_DNA.
DR PIR; A27532; KIVZFP.
DR RefSeq; NP_039049.1; NC_002188.1.
DR SMR; P10052; -.
DR GeneID; 1486634; -.
DR KEGG; vg:1486634; -.
DR Proteomes; UP000008597; Genome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004797; F:thymidine kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0071897; P:DNA biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001267; Thymidine_kinase.
DR InterPro; IPR020633; Thymidine_kinase_CS.
DR PANTHER; PTHR11441; PTHR11441; 1.
DR Pfam; PF00265; TK; 1.
DR PIRSF; PIRSF035805; TK_cell; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00603; TK_CELLULAR_TYPE; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA synthesis; Kinase; Metal-binding; Nucleotide-binding;
KW Reference proteome; Transferase; Zinc.
FT CHAIN 1..183
FT /note="Thymidine kinase"
FT /id="PRO_0000174930"
FT ACT_SITE 89
FT /note="Proton acceptor"
FT /evidence="ECO:0000255"
FT BINDING 11..18
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 119
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 144
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 147
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 163..167
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 176
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 179
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
SQ SEQUENCE 183 AA; 20381 MW; FA60C5629F2DF276 CRC64;
MSSGSIHVIT GPMFSGKTSE LVRRIKRFML SNFKCIIIKH CGDNRYNEDD INKVYTHDLL
FMEATASSNL SVLVPTLLND GVQVIGIDEA QFFLDIVEFS ESMANLGKTV IVAALNGDFK
RELFGNVYKL LSLAETVSSL TAICVKCYCD ASFSKRVTEN KEVMDIGGKD KYIAVCRKCF
FSN
