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KITH_HHV11
ID   KITH_HHV11              Reviewed;         376 AA.
AC   P0DTH5; A0A5J6DX09; G8HBD6; P03176;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   02-JUN-2021, sequence version 1.
DT   03-AUG-2022, entry version 5.
DE   RecName: Full=Thymidine kinase {ECO:0000255|HAMAP-Rule:MF_04029};
DE            EC=2.7.1.21 {ECO:0000255|HAMAP-Rule:MF_04029};
GN   Name=TK {ECO:0000255|HAMAP-Rule:MF_04029}; OrderedLocusNames=UL23;
OS   Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Simplexvirus.
OX   NCBI_TaxID=10299;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=17;
RX   PubMed=2839594; DOI=10.1099/0022-1317-69-7-1531;
RA   McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D.,
RA   Perry L.J., Scott J.E., Taylor P.;
RT   "The complete DNA sequence of the long unique region in the genome of
RT   herpes simplex virus type 1.";
RL   J. Gen. Virol. 69:1531-1574(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=17;
RX   PubMed=22417106; DOI=10.1111/j.1749-6632.2011.06358.x;
RA   Davison A.J.;
RT   "Evolution of sexually transmitted and sexually transmissible human
RT   herpesviruses.";
RL   Ann. N. Y. Acad. Sci. 1230:E37-E49(2011).
RN   [3]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Isolate S17pp, Isolate S17pp22a, Isolate S17pp3, Isolate S17pp4,
RC   Isolate S17pp5, and Isolate S17pp8;
RA   Jones J., Depledge D., Breuer J., Ebert-Keel K., Elliott G.;
RT   "Genetic and phenotypic intrastrain variation in herpes simplex virus type
RT   1 Glasgow strain 17 syn+ derived viruses.";
RL   Submitted (JUL-2019) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=17;
RX   PubMed=32341360; DOI=10.1038/s41467-020-15992-5;
RA   Whisnant A.W., Jurges C.S., Hennig T., Wyler E., Prusty B., Rutkowski A.J.,
RA   L'hernault A., Djakovic L., Gobel M., Doring K., Menegatti J., Antrobus R.,
RA   Matheson N.J., Kunzig F.W.H., Mastrobuoni G., Bielow C., Kempa S.,
RA   Liang C., Dandekar T., Zimmer R., Landthaler M., Grasser F., Lehner P.J.,
RA   Friedel C.C., Erhard F., Dolken L.;
RT   "Integrative functional genomics decodes herpes simplex virus 1.";
RL   Nat. Commun. 11:2038-2038(2020).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 34-376.
RX   PubMed=7628623; DOI=10.1016/0014-5793(95)00680-8;
RA   Wild K., Bohner T., Aubry A., Folkers G., Schulz G.E.;
RT   "The three-dimensional structure of thymidine kinase from herpes simplex
RT   virus type 1.";
RL   FEBS Lett. 368:289-292(1995).
RN   [6]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
RX   PubMed=7552712; DOI=10.1038/nsb1095-876;
RA   Brown D.G., Visse R., Sandhu G., Davies A., Rizkallah P.J., Melitz C.,
RA   Summers W.C., Sanderson M.R.;
RT   "Crystal structures of the thymidine kinase from herpes simplex virus type-
RT   1 in complex with deoxythymidine and ganciclovir.";
RL   Nat. Struct. Biol. 2:876-881(1995).
RN   [7]
RP   X-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS) OF 34-376.
RX   PubMed=9336833; DOI=10.1002/pro.5560061005;
RA   Wild K., Bohner T., Folkers G., Schulz G.E.;
RT   "The structures of thymidine kinase from herpes simplex virus type 1 in
RT   complex with substrates and a substrate analogue.";
RL   Protein Sci. 6:2097-2106(1997).
RN   [8]
RP   X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
RX   PubMed=9715911;
RX   DOI=10.1002/(sici)1097-0134(19980815)32:3<350::aid-prot10>3.0.co;2-8;
RA   Champness J.N., Bennett M.S., Wien F., Visse R., Summers W.C.,
RA   Herdewijn P., de Clerq E., Ostrowski T., Jarvest R.L., Sanderson M.R.;
RT   "Exploring the active site of herpes simplex virus type-1 thymidine kinase
RT   by X-ray crystallography of complexes with aciclovir and other ligands.";
RL   Proteins 32:350-361(1998).
RN   [9]
RP   X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
RX   PubMed=9989588; DOI=10.1016/s0014-5793(98)01619-6;
RA   Bennett M.S., Wien F., Champness J.N., Batuwangala T., Rutherford T.,
RA   Summers W.C., Sun H., Wright G., Sanderson M.R.;
RT   "Structure to 1.9-A resolution of a complex with herpes simplex virus type-
RT   1 thymidine kinase of a novel, non-substrate inhibitor: X-ray
RT   crystallographic comparison with binding of aciclovir.";
RL   FEBS Lett. 443:121-125(1999).
RN   [10]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 45-376 IN COMPLEX WITH ADENINE
RP   ANALOG, CATALYTIC ACTIVITY, AND SUBUNIT.
RX   PubMed=11056041;
RX   DOI=10.1002/1097-0134(20001201)41:4<545::aid-prot110>3.0.co;2-8;
RA   Vogt J., Perozzo R., Pautsch A., Prota A., Schelling P., Pilger B.,
RA   Folkers G., Scapozza L., Schulz G.E.;
RT   "Nucleoside binding site of herpes simplex type 1 thymidine kinase analyzed
RT   by X-ray crystallography.";
RL   Proteins 41:545-553(2000).
CC   -!- FUNCTION: Catalyzes the transfer of the gamma-phospho group of ATP to
CC       thymidine to generate dTMP in the salvage pathway of pyrimidine
CC       synthesis. The dTMP serves as a substrate for DNA polymerase during
CC       viral DNA replication. Allows the virus to be reactivated and to grow
CC       in non-proliferative cells lacking a high concentration of
CC       phosphorylated nucleic acid precursors. {ECO:0000255|HAMAP-
CC       Rule:MF_04029}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + thymidine = ADP + dTMP + H(+); Xref=Rhea:RHEA:19129,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17748, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:63528, ChEBI:CHEBI:456216; EC=2.7.1.21;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_04029,
CC         ECO:0000269|PubMed:11056041};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_04029,
CC       ECO:0000269|PubMed:11056041}.
CC   -!- BIOTECHNOLOGY: Used in molecular biology as a selectable marker to
CC       identify transfected eukaryotic cells. Used in cancer suicide gene
CC       therapy to selectively kill transformed cells.
CC   -!- MISCELLANEOUS: Phosphorylates and thereby activates certain drugs like
CC       acyclovir (ACV), valacyclovir, and famciclovir to a toxic form, that
CC       leads to successful suppression of the infection, while the uninfected
CC       cell does not have this ability because it lacks TK. Mutations in
CC       thymidine kinase may induce HSV resistance to antiviral therapies in
CC       immunocompromised patients. The most frequently observed resistant
CC       strains are unable to express TK and are avirulent in animal models of
CC       disease. Resistance may be acquired less frequently by selecting
CC       variants which no longer recognize ACV or ACV triphosphate as
CC       substrates but which retain normal functions.
CC   -!- SIMILARITY: Belongs to the herpesviridae thymidine kinase family.
CC       {ECO:0000255|HAMAP-Rule:MF_04029}.
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DR   EMBL; X14112; CAA32315.1; -; Genomic_DNA.
DR   EMBL; JN555585; AEQ77053.1; -; Genomic_DNA.
DR   EMBL; MN159376; QEU44927.1; -; Genomic_DNA.
DR   EMBL; MN159377; QEU45002.1; -; Genomic_DNA.
DR   EMBL; MN159378; QEU45078.1; -; Genomic_DNA.
DR   EMBL; MN159379; QEU45154.1; -; Genomic_DNA.
DR   EMBL; MN159381; QEU45306.1; -; Genomic_DNA.
DR   EMBL; MN159382; QEU45382.1; -; Genomic_DNA.
DR   EMBL; BK012101; DAC85562.1; -; Genomic_DNA.
DR   PIR; E30084; KIBE17.
DR   RefSeq; YP_009137097.1; NC_001806.2.
DR   PDB; 1E2H; X-ray; 1.90 A; A/B=46-376.
DR   PDB; 1E2I; X-ray; 1.90 A; A/B=46-376.
DR   PDB; 1E2J; X-ray; 2.50 A; A/B=46-376.
DR   PDB; 1E2K; X-ray; 1.70 A; A/B=46-376.
DR   PDB; 1E2L; X-ray; 2.40 A; A/B=46-376.
DR   PDB; 1E2M; X-ray; 2.20 A; A/B=46-376.
DR   PDB; 1E2N; X-ray; 2.20 A; A/B=46-376.
DR   PDB; 1E2P; X-ray; 2.50 A; A/B=46-376.
DR   PDB; 1KI2; X-ray; 2.20 A; A/B=46-376.
DR   PDB; 1KI3; X-ray; 2.37 A; A/B=46-376.
DR   PDB; 1KI4; X-ray; 2.34 A; A/B=46-376.
DR   PDB; 1KI6; X-ray; 2.37 A; A/B=46-376.
DR   PDB; 1KI7; X-ray; 2.20 A; A/B=46-376.
DR   PDB; 1KI8; X-ray; 2.20 A; A/B=46-376.
DR   PDB; 1KIM; X-ray; 2.14 A; A/B=11-376.
DR   PDB; 1OF1; X-ray; 1.95 A; A/B=1-376.
DR   PDB; 1P7C; X-ray; 2.10 A; A/B=34-376.
DR   PDB; 1QHI; X-ray; 1.90 A; A/B=11-376.
DR   PDB; 1VTK; X-ray; 2.75 A; A=34-376.
DR   PDB; 2KI5; X-ray; 1.90 A; A/B=11-376.
DR   PDB; 2VTK; X-ray; 2.80 A; A=34-376.
DR   PDB; 3F0T; X-ray; 2.00 A; A/B=45-376.
DR   PDB; 3RDP; X-ray; 2.80 A; A/B=46-376.
DR   PDB; 3VTK; X-ray; 3.00 A; A=34-376.
DR   PDB; 4IVP; X-ray; 2.10 A; A/B=46-376.
DR   PDB; 4IVQ; X-ray; 1.90 A; A/B=46-376.
DR   PDB; 4IVR; X-ray; 2.40 A; A/B=46-376.
DR   PDB; 4JBX; X-ray; 2.10 A; A/B=45-376.
DR   PDB; 4JBY; X-ray; 2.00 A; A/B=45-376.
DR   PDB; 4OQL; X-ray; 2.10 A; A/B=45-376.
DR   PDB; 4OQM; X-ray; 2.20 A; A/B=45-376.
DR   PDB; 4OQN; X-ray; 2.30 A; A/B=45-376.
DR   PDB; 4OQX; X-ray; 2.50 A; A/B=45-376.
DR   PDBsum; 1E2H; -.
DR   PDBsum; 1E2I; -.
DR   PDBsum; 1E2J; -.
DR   PDBsum; 1E2K; -.
DR   PDBsum; 1E2L; -.
DR   PDBsum; 1E2M; -.
DR   PDBsum; 1E2N; -.
DR   PDBsum; 1E2P; -.
DR   PDBsum; 1KI2; -.
DR   PDBsum; 1KI3; -.
DR   PDBsum; 1KI4; -.
DR   PDBsum; 1KI6; -.
DR   PDBsum; 1KI7; -.
DR   PDBsum; 1KI8; -.
DR   PDBsum; 1KIM; -.
DR   PDBsum; 1OF1; -.
DR   PDBsum; 1P7C; -.
DR   PDBsum; 1QHI; -.
DR   PDBsum; 1VTK; -.
DR   PDBsum; 2KI5; -.
DR   PDBsum; 2VTK; -.
DR   PDBsum; 3F0T; -.
DR   PDBsum; 3RDP; -.
DR   PDBsum; 3VTK; -.
DR   PDBsum; 4IVP; -.
DR   PDBsum; 4IVQ; -.
DR   PDBsum; 4IVR; -.
DR   PDBsum; 4JBX; -.
DR   PDBsum; 4JBY; -.
DR   PDBsum; 4OQL; -.
DR   PDBsum; 4OQM; -.
DR   PDBsum; 4OQN; -.
DR   PDBsum; 4OQX; -.
DR   SMR; P0DTH5; -.
DR   GeneID; 24271467; -.
DR   KEGG; vg:24271467; -.
DR   Proteomes; UP000009294; Genome.
DR   Proteomes; UP000180758; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004797; F:thymidine kinase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0071897; P:DNA biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0006230; P:TMP biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_04029; HSV_KITH; 1.
DR   InterPro; IPR001889; Herpes_TK.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00693; Herpes_TK; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; DNA synthesis; Early protein; Kinase;
KW   Nucleotide-binding; Reference proteome; Transferase.
FT   CHAIN           1..376
FT                   /note="Thymidine kinase"
FT                   /id="PRO_0000175069"
FT   REGION          1..38
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        83
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04029"
FT   BINDING         56..63
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04029"
FT   BINDING         101
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04029"
FT   BINDING         125
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04029"
FT   BINDING         216
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04029"
FT   BINDING         222
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04029"
FT   STRAND          48..54
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   STRAND          56..61
FT                   /evidence="ECO:0007829|PDB:2KI5"
FT   HELIX           62..69
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   HELIX           70..72
FT                   /evidence="ECO:0007829|PDB:2VTK"
FT   TURN            75..77
FT                   /evidence="ECO:0007829|PDB:4IVP"
FT   STRAND          78..81
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   HELIX           85..89
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   STRAND          92..94
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   HELIX           96..108
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   HELIX           114..139
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   HELIX           140..142
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   STRAND          143..148
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   STRAND          150..152
FT                   /evidence="ECO:0007829|PDB:1KI2"
FT   STRAND          156..162
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   HELIX           165..169
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   HELIX           171..178
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   HELIX           184..192
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   STRAND          202..207
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   HELIX           210..219
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   TURN            222..224
FT                   /evidence="ECO:0007829|PDB:1KI8"
FT   HELIX           229..250
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   HELIX           255..258
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   HELIX           259..262
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   STRAND          276..278
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   HELIX           284..286
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   HELIX           288..292
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   HELIX           295..297
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   STRAND          302..304
FT                   /evidence="ECO:0007829|PDB:4IVQ"
FT   HELIX           306..320
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   STRAND          323..328
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   HELIX           333..343
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   HELIX           344..346
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   STRAND          349..354
FT                   /evidence="ECO:0007829|PDB:1E2K"
FT   HELIX           357..372
FT                   /evidence="ECO:0007829|PDB:1E2K"
SQ   SEQUENCE   376 AA;  40921 MW;  5128181FBF08DA50 CRC64;
     MASYPCHQHA SAFDQAARSR GHNNRRTALR PRRQQKATEV RLEQKMPTLL RVYIDGPHGM
     GKTTTTQLLV ALGSRDDIVY VPEPMTYWRV LGASETIANI YTTQHRLDQG EISAGDAAVV
     MTSAQITMGM PYAVTDAVLA PHIGGEAGSS HAPPPALTLI FDRHPIAALL CYPAARYLMG
     SMTPQAVLAF VALIPPTLPG TNIVLGALPE DRHIDRLAKR QRPGERLDLA MLAAIRRVYG
     LLANTVRYLQ GGGSWREDWG QLSGAAVPPQ GAEPQSNAGP RPHIGDTLFT LFRAPELLAP
     NGDLYNVFAW ALDVLAKRLR PMHVFILDYD QSPAGCRDAL LQLTSGMVQT HVTTPGSIPT
     ICDLARTFAR EMGEAN
 
 
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