ID   KITH_ICHVA              Reviewed;         228 AA.
AC   P28855;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Thymidine kinase;
DE            EC=2.7.1.21;
GN   Name=TK; Synonyms=5;
OS   Ictalurid herpesvirus 1 (strain Auburn) (IcHV-1) (Channel catfish
OS   herpesvirus).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Alloherpesviridae; Ictalurivirus.
OX   NCBI_TaxID=766178;
OH   NCBI_TaxID=7996; Ictaluridae (bullhead catfishes).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=1727613; DOI=10.1016/0042-6822(92)90056-u;
RA   Davison A.J.;
RT   "Channel catfish virus: a new type of herpesvirus.";
RL   Virology 186:9-14(1992).
RN   [2]
RP   DISCUSSION OF SEQUENCE.
RX   PubMed=1919533; DOI=10.1099/0022-1317-72-10-2583;
RA   Harrison P.T., Thompson R., Davison A.J.;
RT   "Evolution of herpesvirus thymidine kinases from cellular deoxycytidine
RT   kinase.";
RL   J. Gen. Virol. 72:2583-2586(1991).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + thymidine = ADP + dTMP + H(+); Xref=Rhea:RHEA:19129,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17748, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:63528, ChEBI:CHEBI:456216; EC=2.7.1.21;
CC   -!- SIMILARITY: Belongs to the DCK/DGK family. {ECO:0000305}.
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DR   EMBL; M75136; AAA88186.1; -; Genomic_DNA.
DR   EMBL; M75136; AAA88108.1; -; Genomic_DNA.
DR   PIR; JQ1336; KIBEIC.
DR   RefSeq; NP_041096.1; NC_001493.2.
DR   RefSeq; NP_041174.1; NC_001493.2.
DR   SMR; P28855; -.
DR   GeneID; 1488381; -.
DR   GeneID; 1488423; -.
DR   KEGG; vg:1488381; -.
DR   KEGG; vg:1488423; -.
DR   Proteomes; UP000007643; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004797; F:thymidine kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071897; P:DNA biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR031314; DNK_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01712; dNK; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA synthesis; Kinase; Nucleotide-binding; Reference proteome;
KW   Transferase.
FT   CHAIN           1..228
FT                   /note="Thymidine kinase"
FT                   /id="PRO_0000175089"
FT   ACT_SITE        50
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
FT   BINDING         23..30
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         68
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         79
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         109
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         157
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   228 AA;  25642 MW;  980C26879787280B CRC64;
     MALREGPTPA RFLPEGLVFC VEGNIGCGKS TLVKALMERV AGSGVNVVEE PVDQWVNHNG
     KNYLELSYTD PTGYAVPFQN LVFDSYVNVQ RLQNPDIMER SPMSATRVFC AVNGSRGVIP
     ATALPGMAAR GEAVMRTIAT RPVFVYLELP PEECLRRMRR RDRTGEAGVG LDYLRLLHER
     YEAWLSSAED VERVDASRSR EEIVDRVIEI LCRRHPRLRA PLTRKSQL