ID   KITH_MONPZ              Reviewed;         177 AA.
AC   P04363; Q8V4Z6;
DT   20-MAR-1987, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2002, sequence version 2.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Thymidine kinase;
DE            EC=2.7.1.21;
GN   Name=TK; ORFNames=L2R;
OS   Monkeypox virus (strain Zaire-96-I-16) (MPX).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=619591;
OH   NCBI_TaxID=45479; Cynomys gunnisoni (Gunnison's prairie dog).
OH   NCBI_TaxID=99825; Cynomys leucurus (White-tailed prairie dog).
OH   NCBI_TaxID=45480; Cynomys ludovicianus (Black-tailed prairie dog).
OH   NCBI_TaxID=99826; Cynomys mexicanus (Mexican prairie dog).
OH   NCBI_TaxID=99827; Cynomys parvidens (Utah prairie dog).
OH   NCBI_TaxID=30650; Gliridae (dormice).
OH   NCBI_TaxID=226685; Heliosciurus ruwenzorii (Ruwenzori sun squirrel).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6330986; DOI=10.1016/0042-6822(84)90212-5;
RA   Esposito J.J., Knight J.C.;
RT   "Nucleotide sequence of the thymidine kinase gene region of monkeypox and
RT   variola viruses.";
RL   Virology 135:561-567(1984).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Isolate Zaire-96-I-16;
RX   PubMed=11734207; DOI=10.1016/s0014-5793(01)03144-1;
RA   Shchelkunov S.N., Totmenin A.V., Babkin I.V., Safronov P.F.,
RA   Ryazankina O.I., Petrov N.A., Gutorov V.V., Uvarova E.A., Mikheev M.V.,
RA   Sisler J.R., Esposito J.J., Jahrling P.B., Moss B., Sandakhchiev L.S.;
RT   "Human monkeypox and smallpox viruses: genomic comparison.";
RL   FEBS Lett. 509:66-70(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + thymidine = ADP + dTMP + H(+); Xref=Rhea:RHEA:19129,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17748, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:63528, ChEBI:CHEBI:456216; EC=2.7.1.21;
CC   -!- SIMILARITY: Belongs to the thymidine kinase family. {ECO:0000305}.
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DR   EMBL; K02025; AAA80529.1; -; Genomic_DNA.
DR   EMBL; AF380138; AAL40544.1; -; Genomic_DNA.
DR   PIR; A00610; KIVZMV.
DR   RefSeq; NP_536513.1; NC_003310.1.
DR   SMR; P04363; -.
DR   GeneID; 928935; -.
DR   KEGG; vg:928935; -.
DR   Proteomes; UP000101269; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004797; F:thymidine kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071897; P:DNA biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001267; Thymidine_kinase.
DR   InterPro; IPR020633; Thymidine_kinase_CS.
DR   PANTHER; PTHR11441; PTHR11441; 1.
DR   Pfam; PF00265; TK; 1.
DR   PIRSF; PIRSF035805; TK_cell; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00603; TK_CELLULAR_TYPE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA synthesis; Kinase; Metal-binding; Nucleotide-binding;
KW   Transferase; Zinc.
FT   CHAIN           1..177
FT                   /note="Thymidine kinase"
FT                   /id="PRO_0000174931"
FT   ACT_SITE        83
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
FT   BINDING         11..18
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         113
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         138
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         141
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         157..161
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         170
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         173
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        14
FT                   /note="L -> F (in Ref. 1; AAA80529)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        90
FT                   /note="I -> V (in Ref. 1; AAA80529)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        153
FT                   /note="A -> T (in Ref. 1; AAA80529)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   177 AA;  19990 MW;  8A7BAAB3238C91D2 CRC64;
     MNGGHIQLII GPMLSGKSTE LIRRVRRYQI AQYKCVTIKY SNDNRYGTGL WTHDKNNFAA
     LEVTKLCDVL EAITDFSVIG IDEGQFFPDI VEFCERMANE GKIVIVAALD GTFQRRPFNN
     ILNLIPLSEM VVKLTAVCMK CFKEASFSKR LGAETEIEII GGNDMYQSVC RKCYIDS