KITH_SWPVK
ID KITH_SWPVK Reviewed; 177 AA.
AC P23335;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=Thymidine kinase;
DE EC=2.7.1.21;
GN Name=TK; ORFNames=SWF8;
OS Swinepox virus (strain Kasza) (SWPV).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Suipoxvirus.
OX NCBI_TaxID=10277;
OH NCBI_TaxID=9823; Sus scrofa (Pig).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1853562; DOI=10.1016/0042-6822(91)90986-l;
RA Feller J.A., Massung R.F., Turner P.C., Gibbs E.P.J., Bockamp E.O.,
RA Beloso A., Talavera A., Vinuela E., Moyer R.W.;
RT "Isolation and molecular characterization of the swinepox virus thymidine
RT kinase gene.";
RL Virology 183:578-585(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1840707; DOI=10.1016/0042-6822(91)90907-s;
RA Schnitzlein W.M., Tripathy D.N.;
RT "Identification and nucleotide sequence of the thymidine kinase gene of
RT swinepox virus.";
RL Virology 181:727-732(1991).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + thymidine = ADP + dTMP + H(+); Xref=Rhea:RHEA:19129,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17748, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:63528, ChEBI:CHEBI:456216; EC=2.7.1.21;
CC -!- SIMILARITY: Belongs to the thymidine kinase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA47894.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; M64000; AAA47894.1; ALT_INIT; Genomic_DNA.
DR EMBL; M59931; AAA47891.1; -; Genomic_DNA.
DR PIR; B37949; KIVZSW.
DR SMR; P23335; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004797; F:thymidine kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0071897; P:DNA biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001267; Thymidine_kinase.
DR InterPro; IPR020633; Thymidine_kinase_CS.
DR PANTHER; PTHR11441; PTHR11441; 1.
DR Pfam; PF00265; TK; 1.
DR PIRSF; PIRSF035805; TK_cell; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00603; TK_CELLULAR_TYPE; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA synthesis; Kinase; Metal-binding; Nucleotide-binding;
KW Transferase; Zinc.
FT CHAIN 1..177
FT /note="Thymidine kinase"
FT /id="PRO_0000174936"
FT ACT_SITE 84
FT /note="Proton acceptor"
FT /evidence="ECO:0000255"
FT BINDING 11..18
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 114
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 139
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 142
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 158..162
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 171
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 174
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
SQ SEQUENCE 177 AA; 20053 MW; 91694E4A1B4CB942 CRC64;
MSCGFIHLIL GPMFSGKSTE LIRLVNRYQI ATYNCRVIKY SKDNRYGNDA VYTHDKCYIS
AVSTDSLFDI KDTLDDVDIV GIDEGQFFND IVEFCEYIAN KGKIVIVAAL DGTYERKPFG
NILNLIPLSE KVTKLNAICM ICHRDASFSK RLSDEKEIEL IGGKEKYLSV CRSCYLT
