KKA1_ECOLX
ID KKA1_ECOLX Reviewed; 271 AA.
AC P00551; Q53299; Q8GNQ0;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Aminoglycoside 3'-phosphotransferase;
DE EC=2.7.1.95;
DE AltName: Full=APH(3')-I;
DE Short=APH(3')I;
DE AltName: Full=Kanamycin kinase, type I;
DE AltName: Full=Neomycin-kanamycin phosphotransferase type I;
GN Name=aphA1; Synonyms=nptI;
OS Escherichia coli.
OG Plasmid Rts1, and Plasmid pIP1518.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TRANSPOSON=Tn903;
RX PubMed=6270337; DOI=10.1016/0022-2836(81)90438-1;
RA Oka A., Sugisaki H., Takanami M.;
RT "Nucleotide sequence of the kanamycin resistance transposon Tn903.";
RL J. Mol. Biol. 147:217-226(1981).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=X;
RX PubMed=2823223; DOI=10.1093/nar/15.19.8111;
RA Vakulenko S., Kalman M., Horvath B., Simoncsits A.;
RT "The nucleotide sequence of an aminoglycoside 3'-phosphotransferase gene
RT from E. coli.";
RL Nucleic Acids Res. 15:8111-8111(1987).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=Rts1;
RX PubMed=2013575; DOI=10.1128/jb.173.8.2600-2607.1991;
RA Mochida S., Tsuchiya H., Mori K., Kaji A.;
RT "Three short fragments of Rts1 DNA are responsible for the temperature-
RT sensitive growth phenotype (Tsg) of host bacteria.";
RL J. Bacteriol. 173:2600-2607(1991).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Press C.M., Mahaffee W.F., Roche M.M.;
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
RC STRAIN=HM69; PLASMID=pIP1518;
RX PubMed=8381641; DOI=10.1128/aac.37.1.78;
RA Menard R., Molinas C., Arthur M., Duval J., Courvalin P., Leclercq R.;
RT "Overproduction of 3'-aminoglycoside phosphotransferase type I confers
RT resistance to tobramycin in Escherichia coli.";
RL Antimicrob. Agents Chemother. 37:78-83(1993).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
RC TRANSPOSON=Tn903;
RX PubMed=6261245; DOI=10.1073/pnas.77.12.7176;
RA Grindley N.D.F., Joyce C.M.;
RT "Genetic and DNA sequence analysis of the kanamycin resistance transposon
RT Tn903.";
RL Proc. Natl. Acad. Sci. U.S.A. 77:7176-7180(1980).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11 AND 184-271.
RC TRANSPOSON=Tn903;
RX PubMed=6271455; DOI=10.1101/sqb.1981.045.01.021;
RA Grindley N.D.F., Joyce C.M.;
RT "Analysis of the structure and function of the kanamycin-resistance
RT transposon Tn903.";
RL Cold Spring Harb. Symp. Quant. Biol. 45:125-133(1981).
RN [8]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-145.
RX PubMed=6269080; DOI=10.1093/nar/9.14.3335;
RA Heidekamp F., Baas P.D., van Boom J.H., Veeneman G.H., Zipursky S.L.,
RA Jansz H.S.;
RT "Construction and characterization of recombinant plasmid DNAs containing
RT sequences of the origin of bacteriophage phi X174 DNA replication.";
RL Nucleic Acids Res. 9:3335-3354(1981).
CC -!- FUNCTION: Resistance to kanamycin and structurally-related
CC aminoglycosides, including amikacin.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + kanamycin A = ADP + H(+) + kanamycin 3'-phosphate;
CC Xref=Rhea:RHEA:24256, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:57909, ChEBI:CHEBI:58214, ChEBI:CHEBI:456216;
CC EC=2.7.1.95;
CC -!- MISCELLANEOUS: This enzyme is encoded by the kanamycin resistance
CC transposon Tn903.
CC -!- SIMILARITY: Belongs to the aminoglycoside phosphotransferase family.
CC {ECO:0000305}.
CC -!- CAUTION: PubMed:2823223 strain is not known and has been termed 'X' in
CC this entry. {ECO:0000305}.
CC -!- CAUTION: Was originally (Ref.4) termed APH(3')IIa whereas it is
CC APH(3')Ia. {ECO:0000305}.
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DR EMBL; V00359; CAA23656.1; -; Genomic_DNA.
DR EMBL; Y00452; CAA68507.1; -; Genomic_DNA.
DR EMBL; M65202; AAA72095.1; -; Unassigned_DNA.
DR EMBL; AF498082; AAN59785.1; -; Genomic_DNA.
DR EMBL; S54065; AAD13871.1; -; Genomic_DNA.
DR EMBL; V00621; CAA23898.1; -; Genomic_DNA.
DR EMBL; M10596; AAA27445.1; -; Genomic_DNA.
DR EMBL; M10597; AAA27446.1; -; Genomic_DNA.
DR EMBL; V00293; CAA23568.1; -; Genomic_DNA.
DR PIR; A00662; PKECT9.
DR RefSeq; WP_000018322.1; NZ_WVVH01000102.1.
DR RefSeq; WP_063841683.1; NG_047429.1.
DR RefSeq; WP_063842148.1; NG_047437.1.
DR RefSeq; YP_001687822.1; NC_004429.1.
DR RefSeq; YP_002891173.1; NC_012690.1.
DR RefSeq; YP_002995708.1; NC_012886.1.
DR RefSeq; YP_008864028.1; NC_022992.1.
DR RefSeq; YP_008864695.1; NC_022996.1.
DR RefSeq; YP_008995202.1; NC_023277.2.
DR RefSeq; YP_008997405.1; NC_023289.2.
DR AlphaFoldDB; P00551; -.
DR SMR; P00551; -.
DR IntAct; P00551; 2.
DR MINT; P00551; -.
DR ChEMBL; CHEMBL3751655; -.
DR DrugBank; DB01172; Kanamycin.
DR KEGG; ag:CAA23656; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008910; F:kanamycin kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR CDD; cd05150; APH; 1.
DR InterPro; IPR002575; Aminoglycoside_PTrfase.
DR InterPro; IPR024165; Kan/Strep_kinase.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR Pfam; PF01636; APH; 1.
DR PIRSF; PIRSF000706; Kanamycin_kin; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; ATP-binding; Kinase; Nucleotide-binding; Plasmid;
KW Transferase; Transposable element.
FT CHAIN 1..271
FT /note="Aminoglycoside 3'-phosphotransferase"
FT /id="PRO_0000204802"
FT ACT_SITE 198
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT VARIANT 27
FT /note="K -> R (in plasmid Rts1 and strain X)"
FT VARIANT 77
FT /note="E -> A (in plasmid Rts1 and strain X)"
FT VARIANT 126
FT /note="V -> A (in plasmid Rts1)"
FT VARIANT 150
FT /note="A -> T (in plasmid Rts1)"
FT VARIANT 247
FT /note="L -> F (in plasmid Rts1)"
FT VARIANT 249
FT /note="Q -> Y (in plasmid Rts1)"
FT CONFLICT 19
FT /note="L -> M (in Ref. 1 and 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 48
FT /note="D -> N (in Ref. 2; CAA68507)"
FT /evidence="ECO:0000305"
FT CONFLICT 120
FT /note="I -> N (in Ref. 2; CAA68507)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 271 AA; 30961 MW; 3B715D9BBE8031B8 CRC64;
MSHIQRETSC SRPRLNSNLD ADLYGYKWAR DNVGQSGATI YRLYGKPDAP ELFLKHGKGS
VANDVTDEMV RLNWLTEFMP LPTIKHFIRT PDDAWLLTTA IPGKTAFQVL EEYPDSGENI
VDALAVFLRR LHSIPVCNCP FNSDRVFRLA QAQSRMNNGL VDASDFDDER NGWPVEQVWK
EMHKLLPFSP DSVVTHGDFS LDNLIFDEGK LIGCIDVGRV GIADRYQDLA ILWNCLGEFS
PSLQKRLFQK YGIDNPDMNK LQFHLMLDEF F
