ID   KKA1_SALTM              Reviewed;         271 AA.
AC   Q03447;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Aminoglycoside 3'-phosphotransferase;
DE            EC=2.7.1.95;
DE   AltName: Full=APH(3')-I;
DE            Short=APH(3')I;
DE   AltName: Full=Kanamycin kinase, type I;
DE   AltName: Full=Neomycin-kanamycin phosphotransferase type I;
OS   Salmonella typhimurium.
OG   Plasmid NTP16.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=90371;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TRANSPOSON=Tn4353;
RX   PubMed=1325061; DOI=10.1016/0147-619x(92)90024-5;
RA   Cannon P.M., Strike P.;
RT   "Complete nucleotide sequence and gene organization of plasmid NTP16.";
RL   Plasmid 27:220-230(1992).
CC   -!- FUNCTION: Resistance to kanamycin and structurally-related
CC       aminoglycosides, including amikacin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + kanamycin A = ADP + H(+) + kanamycin 3'-phosphate;
CC         Xref=Rhea:RHEA:24256, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57909, ChEBI:CHEBI:58214, ChEBI:CHEBI:456216;
CC         EC=2.7.1.95;
CC   -!- SIMILARITY: Belongs to the aminoglycoside phosphotransferase family.
CC       {ECO:0000305}.
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DR   EMBL; L05392; AAA72313.1; -; Genomic_DNA.
DR   PIR; JQ1545; JQ1545.
DR   RefSeq; NP_052154.1; NC_002090.1.
DR   RefSeq; WP_010891090.1; NG_047435.1.
DR   AlphaFoldDB; Q03447; -.
DR   SMR; Q03447; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008910; F:kanamycin kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   CDD; cd05150; APH; 1.
DR   InterPro; IPR002575; Aminoglycoside_PTrfase.
DR   InterPro; IPR024165; Kan/Strep_kinase.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   Pfam; PF01636; APH; 1.
DR   PIRSF; PIRSF000706; Kanamycin_kin; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; ATP-binding; Kinase; Nucleotide-binding; Plasmid;
KW   Transferase; Transposable element.
FT   CHAIN           1..271
FT                   /note="Aminoglycoside 3'-phosphotransferase"
FT                   /id="PRO_0000204803"
FT   ACT_SITE        198
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   271 AA;  30941 MW;  73A38B81CBF28917 CRC64;
     MSHIQRETSC SRPRLNSNMD ADLYGYKWAR DNVGQSGATI DRLYGKPDAP ELFLKHGKGS
     VANDVTDEMV RLNWMTEFMP LPTIKHFIRT PDDAWLLTTA IPGKTAFQVL EEYPDSGENI
     VDALAVFLRR LHSIPVCNCP FNSDRVFRLV QAQSRMNNGL VDASDFDDER NGWPVEQVWK
     EMHKLLPFSP DSVVTHGDFS LDNLIFDEGK LIGCIDVGRV GIADRYQDLA ILWNCLGEFS
     PSLQKRLFQK YGIDNPDMNK LQFTLMLDEF F