ID   KKA3_STAAU              Reviewed;         263 AA.
AC   P0A3Y6; P00554;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 50.
DE   RecName: Full=Aminoglycoside 3'-phosphotransferase;
DE            EC=2.7.1.95;
DE   AltName: Full=APH(3')III;
DE   AltName: Full=Kanamycin kinase, type III;
DE   AltName: Full=Neomycin-kanamycin phosphotransferase type III;
GN   Name=aphA;
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6100986; DOI=10.1093/oxfordjournals.molbev.a040298;
RA   Gray G.S., Fitch W.M.;
RT   "Evolution of antibiotic resistance genes: the DNA sequence of a kanamycin
RT   resistance gene from Staphylococcus aureus.";
RL   Mol. Biol. Evol. 1:57-66(1983).
CC   -!- FUNCTION: Resistance to kanamycin and structurally-related
CC       aminoglycosides, including amikacin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + kanamycin A = ADP + H(+) + kanamycin 3'-phosphate;
CC         Xref=Rhea:RHEA:24256, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57909, ChEBI:CHEBI:58214, ChEBI:CHEBI:456216;
CC         EC=2.7.1.95;
CC   -!- SIMILARITY: Belongs to the aminoglycoside phosphotransferase family.
CC       {ECO:0000305}.
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DR   EMBL; M36771; AAA26596.1; -; Genomic_DNA.
DR   PIR; A26220; PKSAF.
DR   AlphaFoldDB; P0A3Y6; -.
DR   BMRB; P0A3Y6; -.
DR   SMR; P0A3Y6; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008910; F:kanamycin kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   CDD; cd05150; APH; 1.
DR   InterPro; IPR002575; Aminoglycoside_PTrfase.
DR   InterPro; IPR024165; Kan/Strep_kinase.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   Pfam; PF01636; APH; 1.
DR   PIRSF; PIRSF000706; Kanamycin_kin; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; ATP-binding; Kinase; Nucleotide-binding;
KW   Transferase.
FT   CHAIN           1..263
FT                   /note="Aminoglycoside 3'-phosphotransferase"
FT                   /id="PRO_0000204806"
FT   ACT_SITE        189
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   263 AA;  30901 MW;  1FADEAE7B9A2F952 CRC64;
     MAKMRISPEL KKLIEKYRCV KDTEGMSPAK VYKLGENENL YLKMTDSRYK GTTYDVEREK
     DMMLWLEGKL PVPKVVHFER HDGWSNLLMS EADGVLCSEE YEDEQSPEKI IELYAECIRL
     FHSIDISDCP YTNSLDSRLA ELDYLLNNDL ADVDCENWEE DTPFKDPREL YDFLKTEKPE
     EELVFSHGDL GDSKIFVKDG KVSGFIDLGR SGRADKWYDI PFCVRSIRED IGEEQYVELF
     FDLLGIKPDW EKIKYYILLD ELF