ID   KKA4_NIACI              Reviewed;         262 AA.
AC   P00553;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Aminoglycoside 3'-phosphotransferase {ECO:0000303|PubMed:3006668, ECO:0000303|PubMed:6193008};
DE            EC=2.7.1.95;
DE   AltName: Full=APH(3')IV {ECO:0000303|PubMed:7522196};
DE   AltName: Full=Butirosin resistance protein A {ECO:0000303|PubMed:7522196};
DE   AltName: Full=Kanamycin kinase, type IV;
DE   AltName: Full=Neomycin-kanamycin phosphotransferase type IV;
GN   Name=aphA4 {ECO:0000303|PubMed:7522196};
GN   Synonyms=aph {ECO:0000303|PubMed:3006668, ECO:0000303|PubMed:6193008},
GN   butA {ECO:0000303|PubMed:7522196};
OS   Niallia circulans (Bacillus circulans).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Niallia.
OX   NCBI_TaxID=1397;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3006668; DOI=10.1042/bj2330383;
RA   Herbert C.J., Sarwar M., Ner S.S., Giles I.G., Akhtar M.;
RT   "Sequence and interspecies transfer of an aminoglycoside phosphotransferase
RT   gene (APH) of Bacillus circulans. Self-defence mechanism in antibiotic-
RT   producing organisms.";
RL   Biochem. J. 233:383-393(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6193008; DOI=10.1016/0014-5793(83)80937-5;
RA   Herbert C.J., Giles I.G., Akhtar M.;
RT   "The sequence of an antibiotic resistance gene from an antibiotic-producing
RT   bacterium. Homologies with transposon genes.";
RL   FEBS Lett. 160:67-71(1983).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25.
RC   STRAIN=BCRC 11491 / NRRL B-3312;
RX   PubMed=7522196; DOI=10.1016/0378-1119(94)90032-9;
RA   Aubert-Pivert E., Davies J.;
RT   "Biosynthesis of butirosin in Bacillus circulans NRRL B3312: identification
RT   by sequence analysis and insertional mutagenesis of the butB gene involved
RT   in antibiotic production.";
RL   Gene 147:1-11(1994).
RN   [4]
RP   PROTEIN SEQUENCE OF 1-12.
RX   PubMed=2163618; DOI=10.1042/bj2680671;
RA   Sarwar M., Akhtar M.;
RT   "Cloning of aminoglycoside phosphotransferase (APH) gene from antibiotic-
RT   producing strain of Bacillus circulans into a high-expression vector,
RT   pKK223-3. Purification, properties and location of the enzyme.";
RL   Biochem. J. 268:671-677(1990).
CC   -!- FUNCTION: Resistance to butirosin and structurally-related
CC       aminoglycosides, including kanamycin and amikacin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + kanamycin A = ADP + H(+) + kanamycin 3'-phosphate;
CC         Xref=Rhea:RHEA:24256, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57909, ChEBI:CHEBI:58214, ChEBI:CHEBI:456216;
CC         EC=2.7.1.95;
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the aminoglycoside phosphotransferase family.
CC       {ECO:0000305}.
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DR   EMBL; X03364; CAA27061.1; -; Genomic_DNA.
DR   EMBL; L20421; AAA62589.1; -; Genomic_DNA.
DR   PIR; A00664; PKBSK.
DR   RefSeq; WP_063841674.1; NG_047428.1.
DR   AlphaFoldDB; P00553; -.
DR   SMR; P00553; -.
DR   KEGG; ag:CAA27061; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008910; F:kanamycin kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   CDD; cd05150; APH; 1.
DR   InterPro; IPR002575; Aminoglycoside_PTrfase.
DR   InterPro; IPR024165; Kan/Strep_kinase.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   Pfam; PF01636; APH; 1.
DR   PIRSF; PIRSF000706; Kanamycin_kin; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; ATP-binding; Cytoplasm; Direct protein sequencing;
KW   Kinase; Nucleotide-binding; Transferase.
FT   CHAIN           1..262
FT                   /note="Aminoglycoside 3'-phosphotransferase"
FT                   /id="PRO_0000204807"
FT   ACT_SITE        187
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        3
FT                   /note="E -> Q (in Ref. 4; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        106
FT                   /note="K -> N (in Ref. 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   262 AA;  29913 MW;  D0D0998B4974C3B3 CRC64;
     MNESTRNWPE ELLELLGQTE LTVNKIGYSG DHVYHVKEYR GTPAFLKIAP SVWWRTLRPE
     IEALAWLDGK LPVPKILYTA EHGGMDYLLM EALGGKDGSH ETIQAKRKLF VKLYAEGLRS
     VHGLDIRECP LSNGLEKKLR DAKRIVDESL VDPADIKEEY DCTPEELYGL LLESKPVTED
     LVFAHGDYCA PNLIIDGEKL SGFIDLGRAG VADRYQDISL AIRSLRHDYG DDRYKALFLE
     LYGLDGLDED KVRYYIRLDE FF