ID   KKA5_STRFR              Reviewed;         268 AA.
AC   P00555;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Aminoglycoside 3'-phosphotransferase;
DE            EC=2.7.1.95;
DE   AltName: Full=APH(3')V;
DE   AltName: Full=Kanamycin kinase, type V;
DE   AltName: Full=Neomycin-kanamycin phosphotransferase type V;
GN   Name=aph;
OS   Streptomyces fradiae (Streptomyces roseoflavus).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1906;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 10745 / CBS 498.68 / DSM 40063 / JCM 4133 / NBRC 12773 / NCIMB
RC   8233 / NRRL B-1195 / VKM Ac-150;
RX   PubMed=6310563; DOI=10.1073/pnas.80.17.5190;
RA   Thompson C.J., Gray G.S.;
RT   "Nucleotide sequence of a streptomycete aminoglycoside phosphotransferase
RT   gene and its relationship to phosphotransferases encoded by resistance
RT   plasmids.";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:5190-5194(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-71.
RX   PubMed=2987648; DOI=10.1007/bf00327505;
RA   Bibb M.J., Bibb M.J., Ward J.M., Cohen S.N.;
RT   "Nucleotide sequences encoding and promoting expression of three antibiotic
RT   resistance genes indigenous to Streptomyces.";
RL   Mol. Gen. Genet. 199:26-36(1985).
CC   -!- FUNCTION: Resistance to kanamycin and structurally-related
CC       aminoglycosides, including amikacin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + kanamycin A = ADP + H(+) + kanamycin 3'-phosphate;
CC         Xref=Rhea:RHEA:24256, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57909, ChEBI:CHEBI:58214, ChEBI:CHEBI:456216;
CC         EC=2.7.1.95;
CC   -!- SIMILARITY: Belongs to the aminoglycoside phosphotransferase family.
CC       {ECO:0000305}.
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DR   EMBL; K00432; AAA26699.1; -; Genomic_DNA.
DR   EMBL; X02394; CAA26236.1; -; Genomic_DNA.
DR   EMBL; A04924; CAA00404.1; -; Unassigned_DNA.
DR   PIR; A00666; PKSMR.
DR   RefSeq; WP_063842176.1; NG_047450.1.
DR   AlphaFoldDB; P00555; -.
DR   SMR; P00555; -.
DR   KEGG; ag:AAA26699; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008910; F:kanamycin kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   CDD; cd05150; APH; 1.
DR   InterPro; IPR002575; Aminoglycoside_PTrfase.
DR   InterPro; IPR024165; Kan/Strep_kinase.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   Pfam; PF01636; APH; 1.
DR   PIRSF; PIRSF000706; Kanamycin_kin; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; ATP-binding; Kinase; Nucleotide-binding;
KW   Transferase.
FT   CHAIN           1..268
FT                   /note="Aminoglycoside 3'-phosphotransferase"
FT                   /id="PRO_0000204808"
FT   ACT_SITE        188
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   268 AA;  29986 MW;  868C622038FE1C14 CRC64;
     MDDSTLRRKY PHHEWHAVNE GDSGAFVYQL TGGPEPQPEL YAKIAPRAPE NSAFDLSGEA
     DRLEWLHRHG IPVPRVVERG ADDTAAWLVT EAVPGVAAAE EWPEHQRFAV VEAMAELARA
     LHELPVEDCP SDRRLDAAVA EARRNVAEGL VDLDDLQEER AGWTGDQLLA ELDRTRPEKE
     DLVVCHGDLC PNNVLLDPGT CRVTGVIDVG RLGVADRHAD IALAARELEI DEDPWFGPAY
     AERFLERYGA HRVDKEKLAF YQLLDEFF