ID   KKA6_ACIBA              Reviewed;         259 AA.
AC   P09885;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Aminoglycoside 3'-phosphotransferase;
DE            EC=2.7.1.95;
DE   AltName: Full=APH(3')VI;
DE   AltName: Full=Kanamycin kinase, type VI;
DE   AltName: Full=Neomycin-kanamycin phosphotransferase type VI;
GN   Name=aphA-6;
OS   Acinetobacter baumannii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Moraxellales; Moraxellaceae;
OC   Acinetobacter; Acinetobacter calcoaceticus/baumannii complex.
OX   NCBI_TaxID=470;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BM2580;
RX   PubMed=2846986; DOI=10.1111/j.1365-2958.1988.tb00070.x;
RA   Martin P., Jullien E., Courvalin P.;
RT   "Nucleotide sequence of Acinetobacter baumannii aphA-6 gene: evolutionary
RT   and functional implications of sequence homologies with nucleotide-binding
RT   proteins, kinases and other aminoglycoside-modifying enzymes.";
RL   Mol. Microbiol. 2:615-625(1988).
CC   -!- FUNCTION: Resistance to kanamycin and structurally-related
CC       aminoglycosides, including amikacin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + kanamycin A = ADP + H(+) + kanamycin 3'-phosphate;
CC         Xref=Rhea:RHEA:24256, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57909, ChEBI:CHEBI:58214, ChEBI:CHEBI:456216;
CC         EC=2.7.1.95;
CC   -!- SIMILARITY: Belongs to the aminoglycoside phosphotransferase family.
CC       {ECO:0000305}.
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DR   EMBL; X07753; CAA30578.1; -; Genomic_DNA.
DR   PIR; S01928; S01928.
DR   RefSeq; WP_000422636.1; NZ_WYAS01000090.1.
DR   RefSeq; YP_009066658.1; NC_025111.1.
DR   AlphaFoldDB; P09885; -.
DR   SMR; P09885; -.
DR   KEGG; ag:CAA30578; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008910; F:kanamycin kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   CDD; cd05150; APH; 1.
DR   InterPro; IPR002575; Aminoglycoside_PTrfase.
DR   InterPro; IPR024165; Kan/Strep_kinase.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   Pfam; PF01636; APH; 1.
DR   PIRSF; PIRSF000706; Kanamycin_kin; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; ATP-binding; Kinase; Nucleotide-binding;
KW   Transferase.
FT   CHAIN           1..259
FT                   /note="Aminoglycoside 3'-phosphotransferase"
FT                   /id="PRO_0000204809"
FT   ACT_SITE        187
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   259 AA;  30327 MW;  72071BCE23E3DAED CRC64;
     MELPNIIQQF IGNSVLEPNK IGQSPSDVYS FNRNNETFFL KRSSTLYTET TYSVSREAKM
     LSWLSEKLKV PELIMTFQDE QFEFMITKAI NAKPISALFL TDQELLAIYK EALNLLNSIA
     IIDCPFISNI DHRLKESKFF IDNQLLDDID QDDFDTELWG DHKTYLSLWN ELTETRVEER
     LVFSHGDITD SNIFIDKFNE IYFLDLGRAG LADEFVDISF VERCLREDAS EETAKIFLKH
     LKNDRPDKRN YFLKLDELN