ID   KKA8_ECOLX              Reviewed;         271 AA.
AC   P14509;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Aminoglycoside 3'-phosphotransferase;
DE            EC=2.7.1.95;
DE   AltName: Full=APH(3')VIII;
DE   AltName: Full=Kanamycin kinase, type VIII;
DE   AltName: Full=Neomycin-kanamycin phosphotransferase type VIII;
GN   Name=aphA;
OS   Escherichia coli.
OG   Plasmid IncP-alpha RP4.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2832861; DOI=10.1016/0147-619x(87)90062-x;
RA   Pansegrau W., Miele L., Lurz R., Lanka E.;
RT   "Nucleotide sequence of the kanamycin resistance determinant of plasmid
RT   RP4: homology to other aminoglycoside 3'-phosphotransferases.";
RL   Plasmid 18:193-204(1987).
CC   -!- FUNCTION: Resistance to kanamycin and structurally-related
CC       aminoglycosides, including amikacin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + kanamycin A = ADP + H(+) + kanamycin 3'-phosphate;
CC         Xref=Rhea:RHEA:24256, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57909, ChEBI:CHEBI:58214, ChEBI:CHEBI:456216;
CC         EC=2.7.1.95;
CC   -!- SIMILARITY: Belongs to the aminoglycoside phosphotransferase family.
CC       {ECO:0000305}.
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DR   EMBL; M20305; AAA26412.1; -; Genomic_DNA.
DR   PIR; JU0103; JU0103.
DR   AlphaFoldDB; P14509; -.
DR   SMR; P14509; -.
DR   IntAct; P14509; 1.
DR   KEGG; ag:AAA26412; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008910; F:kanamycin kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   CDD; cd05150; APH; 1.
DR   InterPro; IPR002575; Aminoglycoside_PTrfase.
DR   InterPro; IPR024165; Kan/Strep_kinase.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   Pfam; PF01636; APH; 1.
DR   PIRSF; PIRSF000706; Kanamycin_kin; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; ATP-binding; Kinase; Nucleotide-binding; Plasmid;
KW   Transferase.
FT   CHAIN           1..271
FT                   /note="Aminoglycoside 3'-phosphotransferase"
FT                   /id="PRO_0000204811"
FT   ACT_SITE        198
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   271 AA;  30105 MW;  A489F64B00E8F313 CRC64;
     MNDIDREEPC AAAAVPESMA AHVMGYKWAR DKVGQSGCAV YRLHSKSGGS DLFLKHGKDA
     FADDVTDEMV RLRWLAGHIS VPSVVSFVRT PNQAWLLTTA IHGKTAYQVL KSDFGARLVV
     VDALAAFMRR LHAIPVSECS VQQWTTHAGL PERGSIEAGV VDVDDFDKER EGWTAEQVWE
     AMHRLLPLAP DPVVTHGDFS LDNLLIVEGK VVGCIDVGRA GIADRYQDLA VLWNCLEEFE
     PSLQERLVAQ YGIADPDRRK LQFHLLLDEL F