ID   KKX28_HETPE             Reviewed;          61 AA.
AC   P0DJ35;
DT   18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2012, sequence version 1.
DT   25-MAY-2022, entry version 18.
DE   RecName: Full=Potassium channel toxin kappa-KTx 2.8 {ECO:0000303|PubMed:22305749};
DE   AltName: Full=HSP053C.2 {ECO:0000303|PubMed:20443192};
DE   AltName: Full=Toxin HeTx204 {ECO:0000303|PubMed:22511981};
DE   AltName: Full=Toxin kappa-KTx 2.7 {ECO:0000303|PubMed:22511981};
DE   Flags: Precursor; Fragment;
OS   Heterometrus petersii (Asian forest scorpion).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Iurida; Scorpionoidea; Scorpionidae; Heterometrinae;
OC   Heterometrus.
OX   NCBI_TaxID=754296;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=20443192; DOI=10.1002/pmic.200900763;
RA   Ma Y., Zhao Y., Zhao R., Zhang W., He Y., Wu Y., Cao Z., Guo L., Li W.;
RT   "Molecular diversity of toxic components from the scorpion Heterometrus
RT   petersii venom revealed by proteomic and transcriptome analysis.";
RL   Proteomics 10:2471-2485(2010).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC   TISSUE=Venom gland;
RX   PubMed=22511981; DOI=10.1371/journal.pone.0035154;
RA   Chen Z.Y., Zeng D.Y., Hu Y.T., He Y.W., Pan N., Ding J.P., Cao Z.J.,
RA   Liu M.L., Li W.X., Yi H., Jiang L., Wu Y.L.;
RT   "Structural and functional diversity of acidic scorpion potassium channel
RT   toxins.";
RL   PLoS ONE 7:E35154-E35154(2012).
RN   [3]
RP   NOMENCLATURE.
RX   PubMed=22305749; DOI=10.1016/j.bcp.2012.01.021;
RA   Vandendriessche T., Kopljar I., Jenkins D.P., Diego-Garcia E.,
RA   Abdel-Mottaleb Y., Vermassen E., Clynen E., Schoofs L., Wulff H.,
RA   Snyders D., Tytgat J.;
RT   "Purification, molecular cloning and functional characterization of HelaTx1
RT   (Heterometrus laoticus): the first member of a new kappa-KTX subfamily.";
RL   Biochem. Pharmacol. 83:1307-1317(2012).
CC   -!- FUNCTION: Voltage-gated potassium channel inhibitor (Kv) that acts on
CC       Kv1.3/KCNA3 and Kv7.1/KCNQ1. 1 uM of the toxin inhibits Kv1.3/KCNA3
CC       currents by 35.1%, whereas 10 uM of the toxin inhibits Kv7.1/KCNQ1
CC       currents by 44.9%. {ECO:0000269|PubMed:22511981}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: Has the structural arrangement of two alpha-helices stabilized
CC       by disulfide bonds (CSalpha/alpha 2(S-S)).
CC       {ECO:0000250|UniProtKB:P0C1Z3}.
CC   -!- MISCELLANEOUS: Does not show activity on ion channels KCa2.3/KCNN3,
CC       TRPV1, and KCa1.1/KCNMA1. {ECO:0000305|PubMed:22511981}.
CC   -!- SIMILARITY: Belongs to the short scorpion toxin superfamily. Potassium
CC       channel inhibitor kappa-KTx family. Kappa-KTx 2 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; FD664204; -; NOT_ANNOTATED_CDS; mRNA.
DR   AlphaFoldDB; P0DJ35; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Disulfide bond;
KW   Ion channel impairing toxin; Neurotoxin; Potassium channel impairing toxin;
KW   Secreted; Signal; Toxin; Voltage-gated potassium channel impairing toxin.
FT   SIGNAL          <1..21
FT                   /evidence="ECO:0000255"
FT   PROPEP          22..35
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000416800"
FT   PEPTIDE         38..61
FT                   /note="Potassium channel toxin kappa-KTx 2.8"
FT                   /id="PRO_0000416801"
FT   DISULFID        41..59
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z3"
FT   DISULFID        45..55
FT                   /evidence="ECO:0000250|UniProtKB:P0C1Z3"
FT   NON_TER         1
SQ   SEQUENCE   61 AA;  6748 MW;  E31C93C933D42CBE CRC64;
     GTVYVFLLLL AFGIFTDISN ACSEQMDDED SYEVEKRGNA CIEVCLQHTG NPAECDKPCD
     K