ARAE2_ARATH
ID ARAE2_ARATH Reviewed; 417 AA.
AC O64749; Q8VYK0;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-MAR-2006, sequence version 3.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Putative UDP-arabinose 4-epimerase 2;
DE EC=5.1.3.5;
DE AltName: Full=UDP-D-xylose 4-epimerase 2;
GN OrderedLocusNames=At2g34850; ORFNames=F19I3.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=UDP-beta-L-arabinopyranose = UDP-alpha-D-xylose;
CC Xref=Rhea:RHEA:11320, ChEBI:CHEBI:57632, ChEBI:CHEBI:61457;
CC EC=5.1.3.5;
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC -!- PATHWAY: Nucleotide-sugar biosynthesis; UDP-L-arabinose biosynthesis;
CC UDP-L-arabinose from UDP-alpha-D-xylose: step 1/1.
CC -!- PATHWAY: Cell wall biogenesis; cell wall polysaccharide biosynthesis.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane
CC {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O64749-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O64749-2; Sequence=VSP_017346;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing donor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC12825.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC004238; AAC12825.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC09030.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62283.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62284.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62285.1; -; Genomic_DNA.
DR EMBL; AY070487; AAL49952.1; -; mRNA.
DR EMBL; AY091699; AAM10298.1; -; mRNA.
DR PIR; T00467; T00467.
DR RefSeq; NP_001324451.1; NM_001336532.1. [O64749-2]
DR RefSeq; NP_001324452.1; NM_001336531.1. [O64749-2]
DR RefSeq; NP_001324453.1; NM_001336533.1. [O64749-2]
DR RefSeq; NP_850238.1; NM_179907.5. [O64749-2]
DR AlphaFoldDB; O64749; -.
DR SMR; O64749; -.
DR STRING; 3702.AT2G34850.1; -.
DR iPTMnet; O64749; -.
DR PaxDb; O64749; -.
DR PeptideAtlas; O64749; -.
DR PRIDE; O64749; -.
DR ProteomicsDB; 240606; -. [O64749-1]
DR EnsemblPlants; AT2G34850.1; AT2G34850.1; AT2G34850. [O64749-2]
DR EnsemblPlants; AT2G34850.2; AT2G34850.2; AT2G34850. [O64749-2]
DR EnsemblPlants; AT2G34850.3; AT2G34850.3; AT2G34850. [O64749-2]
DR EnsemblPlants; AT2G34850.4; AT2G34850.4; AT2G34850. [O64749-2]
DR GeneID; 818050; -.
DR Gramene; AT2G34850.1; AT2G34850.1; AT2G34850. [O64749-2]
DR Gramene; AT2G34850.2; AT2G34850.2; AT2G34850. [O64749-2]
DR Gramene; AT2G34850.3; AT2G34850.3; AT2G34850. [O64749-2]
DR Gramene; AT2G34850.4; AT2G34850.4; AT2G34850. [O64749-2]
DR KEGG; ath:AT2G34850; -.
DR Araport; AT2G34850; -.
DR TAIR; locus:2044802; AT2G34850.
DR eggNOG; KOG1371; Eukaryota.
DR InParanoid; O64749; -.
DR OMA; HIVAMEK; -.
DR OrthoDB; 662484at2759; -.
DR PhylomeDB; O64749; -.
DR BioCyc; ARA:AT2G34850-MON; -.
DR BRENDA; 5.1.3.5; 399.
DR UniPathway; UPA00797; UER00772.
DR UniPathway; UPA00963; -.
DR PRO; PR:O64749; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O64749; baseline and differential.
DR GO; GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0050373; F:UDP-arabinose 4-epimerase activity; IEA:UniProtKB-EC.
DR GO; GO:0003978; F:UDP-glucose 4-epimerase activity; IEA:InterPro.
DR GO; GO:0045227; P:capsule polysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0006012; P:galactose metabolic process; IEA:InterPro.
DR GO; GO:0048868; P:pollen tube development; IMP:TAIR.
DR GO; GO:0033358; P:UDP-L-arabinose biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd05247; UDP_G4E_1_SDR_e; 1.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR005886; UDP_G4E.
DR Pfam; PF16363; GDP_Man_Dehyd; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
DR TIGRFAMs; TIGR01179; galE; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Carbohydrate metabolism; Golgi apparatus; Isomerase;
KW Membrane; NAD; Reference proteome; Signal-anchor; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..417
FT /note="Putative UDP-arabinose 4-epimerase 2"
FT /id="PRO_0000183230"
FT TOPO_DOM 1..31
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..54
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 55..417
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT ACT_SITE 219
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 71..102
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..181
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_017346"
SQ SEQUENCE 417 AA; 45955 MW; CCAA8F9F15F6503C CRC64;
MLNLGRARTG RQNRSMSFEG LDFADPKKNN NYMGKIVLVM TLTAMCILLL NQSPTFNTPS
VFSRSEPGVT HVLVTGGAGY IGSHAALRLL KDSYRVTIVD NLSRGNLGAV KILQQLFPEP
GKLQFIYADL GDANAVNKIF SENAFDAVMH FAAVAYVGES TQFPLKYYHN ITSNTLVVLE
TMAAHGVKTL IYSSTCATYG EPEKMPITEE TPQVPINPYG KAKKMAEDII LDFSKNSIMA
VMILRYFNVI GSDPEGRLGE APRPELSEHG RISGACFDAA RGIIPGLQIK GTDYKTVDGT
CVRDYIDVTD LVDAHVKALE KAKPRKVGIF NVGTGKGSSV KEFVEACKKA TGVDIKVDYL
ERRAGDYAEV YSDPRKIKEE LNWTAKHTNL QESLKMAWRW QKLHRSGYGS SSLVSAY
