ID   KLCA2_ECOLX             Reviewed;         146 AA.
AC   P52603; Q47333;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 46.
DE   RecName: Full=Antirestriction protein KlcA;
GN   Name=klcA; Synonyms=kilC;
OS   Escherichia coli.
OG   Plasmid IncP-alpha RK2.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7519596; DOI=10.1128/jb.176.16.5022-5032.1994;
RA   Larsen M.H., Figurski D.H.;
RT   "Structure, expression, and regulation of the kilC operon of promiscuous
RT   IncP alpha plasmids.";
RL   J. Bacteriol. 176:5022-5032(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7773415; DOI=10.1099/13500872-141-5-1201;
RA   Thomas C.M., Smith C.A., Ibbotson J.P., Johnston L., Wang N.;
RT   "Evolution of the korA-oriV segment of promiscuous IncP plasmids.";
RL   Microbiology 141:1201-1210(1995).
CC   -!- FUNCTION: Could be involved in overcoming restriction barriers during
CC       establishment after conjugative transfer.
CC   -!- INDUCTION: Expression is decreased in the presence of KorA and KorC.
CC   -!- SIMILARITY: Belongs to the antirestriction protein family.
CC       {ECO:0000305}.
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DR   EMBL; U05773; AAA57448.1; -; Genomic_DNA.
DR   EMBL; L13392; AAA75284.1; -; Genomic_DNA.
DR   PIR; A55857; A55857.
DR   PIR; I41323; I41323.
DR   RefSeq; WP_011205777.1; NZ_NJKC01000035.1.
DR   AlphaFoldDB; P52603; -.
DR   SMR; P52603; -.
DR   IntAct; P52603; 1.
DR   Gene3D; 3.30.70.3580; -; 1.
DR   InterPro; IPR004914; Antirestrict.
DR   InterPro; IPR042297; Antirestriction_sf.
DR   Pfam; PF03230; Antirestrict; 1.
PE   2: Evidence at transcript level;
KW   Plasmid.
FT   CHAIN           1..146
FT                   /note="Antirestriction protein KlcA"
FT                   /id="PRO_0000068376"
FT   CONFLICT        4
FT                   /note="V -> VMF (in Ref. 2; AAA75284)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        24
FT                   /note="L -> P (in Ref. 2; AAA75284)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        33
FT                   /note="L -> P (in Ref. 2; AAA75284)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        77
FT                   /note="R -> P (in Ref. 2; AAA75284)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   146 AA;  15867 MW;  9835588E26B8BA60 CRC64;
     MTDVQIPSPI VATRVAEADR LRFLPTYFGP SMLRMLRGEA LVFGWMGRLC AAYHGGFWHF
     YTLSNGGFYM APEHDGRLRI EVDGNGFAGE LSADAAGIVA TLFALNQLCA ELAGTADADA
     LIDRYHHLAA FASEHAEAAA IYRAID