KLD8B_MOUSE
ID KLD8B_MOUSE Reviewed; 354 AA.
AC Q9D2D9;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Kelch domain-containing protein 8B;
GN Name=Klhdc8b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Olfactory epithelium;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Involved in pinching off the separated nuclei at the cleavage
CC furrow and in cytokinesis. Required for mitotic integrity and
CC maintenance of chromosomal stability. Protects cells against mitotic
CC errors, centrosomal amplification, micronucleus formation and
CC aneuploidy. Plays a key role of midbody function involving abscission
CC of the daughter cells during cytokinesis and appropriate chromosomal
CC and nuclear segregation into the daughter cells.
CC {ECO:0000250|UniProtKB:Q8IXV7}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q8IXV7}. Midbody
CC {ECO:0000250|UniProtKB:Q8IXV7}. Note=In mitotic cells, concentrates in
CC the midbody of the cytoplasmic bridge linking daughter cells as they
CC are about to separate during cytokinesis.
CC {ECO:0000250|UniProtKB:Q8IXV7}.
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DR EMBL; AK019849; BAB31879.1; -; mRNA.
DR EMBL; AK154428; BAE32579.1; -; mRNA.
DR EMBL; BC048918; AAH48918.1; -; mRNA.
DR CCDS; CCDS23526.1; -.
DR RefSeq; NP_084351.1; NM_030075.2.
DR RefSeq; XP_017169186.1; XM_017313697.1.
DR AlphaFoldDB; Q9D2D9; -.
DR SMR; Q9D2D9; -.
DR IntAct; Q9D2D9; 1.
DR STRING; 10090.ENSMUSP00000035232; -.
DR iPTMnet; Q9D2D9; -.
DR PhosphoSitePlus; Q9D2D9; -.
DR MaxQB; Q9D2D9; -.
DR PaxDb; Q9D2D9; -.
DR PRIDE; Q9D2D9; -.
DR ProteomicsDB; 263616; -.
DR Antibodypedia; 2038; 149 antibodies from 24 providers.
DR DNASU; 78267; -.
DR Ensembl; ENSMUST00000035232; ENSMUSP00000035232; ENSMUSG00000032609.
DR Ensembl; ENSMUST00000193286; ENSMUSP00000142325; ENSMUSG00000032609.
DR Ensembl; ENSMUST00000193895; ENSMUSP00000141453; ENSMUSG00000032609.
DR GeneID; 78267; -.
DR KEGG; mmu:78267; -.
DR UCSC; uc009rpo.1; mouse.
DR CTD; 200942; -.
DR MGI; MGI:1925517; Klhdc8b.
DR VEuPathDB; HostDB:ENSMUSG00000032609; -.
DR eggNOG; KOG1072; Eukaryota.
DR GeneTree; ENSGT00940000160815; -.
DR HOGENOM; CLU_046864_0_0_1; -.
DR InParanoid; Q9D2D9; -.
DR OMA; TRFPCIP; -.
DR OrthoDB; 933937at2759; -.
DR PhylomeDB; Q9D2D9; -.
DR BioGRID-ORCS; 78267; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Klhdc8b; mouse.
DR PRO; PR:Q9D2D9; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q9D2D9; protein.
DR Bgee; ENSMUSG00000032609; Expressed in dorsal pancreas and 191 other tissues.
DR ExpressionAtlas; Q9D2D9; baseline and differential.
DR Genevisible; Q9D2D9; MM.
DR GO; GO:0110070; C:cellularization cleavage furrow; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0045171; C:intercellular bridge; ISS:UniProtKB.
DR GO; GO:0030496; C:midbody; ISS:UniProtKB.
DR GO; GO:1902410; P:mitotic cytokinetic process; ISS:UniProtKB.
DR GO; GO:0140014; P:mitotic nuclear division; ISS:UniProtKB.
DR GO; GO:0098813; P:nuclear chromosome segregation; ISS:UniProtKB.
DR Gene3D; 2.120.10.80; -; 2.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR Pfam; PF01344; Kelch_1; 4.
DR SMART; SM00612; Kelch; 6.
DR SUPFAM; SSF117281; SSF117281; 2.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Cytoplasm; Kelch repeat; Reference proteome;
KW Repeat.
FT CHAIN 1..354
FT /note="Kelch domain-containing protein 8B"
FT /id="PRO_0000119132"
FT REPEAT 1..31
FT /note="Kelch 1"
FT REPEAT 32..79
FT /note="Kelch 2"
FT REPEAT 81..127
FT /note="Kelch 3"
FT REPEAT 128..175
FT /note="Kelch 4"
FT REPEAT 176..222
FT /note="Kelch 5"
FT REPEAT 224..281
FT /note="Kelch 6"
FT REPEAT 282..329
FT /note="Kelch 7"
FT REPEAT 331..354
FT /note="Kelch 8"
SQ SEQUENCE 354 AA; 37592 MW; C4C6D88B61A4FDC5 CRC64;
MAAGGGRAFA WQVFPPMPTC RVYGTVAHQD GHLLVLGGCG RAGLPLDTAE TLDMGSHTWL
ALAPLPTARA GAAAVVLGKQ VLVVGGVDEV QSPVAAVEAF LADEGRWERR ATLPQAAMGV
ATVERDGMVY ALGGMGPDTA PQAQVLVYEP RRDCWLSLPS MPTPCYGAST FLHGNKIYVL
GGRQGKLPVT AFEAFDLETR TWTRHPSLPS RRAFAGCAMA EGSVFSLGGL QQPGPHNFYS
RPHFVNTVEM FDLEHGSWTK LPRSLRMRDK RADFVVGSLG GNIVAIGGLG NQPCPLASVE
SFSLARRRWE VLPAMPTARC SCSSLQAGPR LFVIGGVAQG PSQAVEALCL RDGV
