ID   KLD8B_RAT               Reviewed;         354 AA.
AC   Q5XIA9;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Kelch domain-containing protein 8B;
GN   Name=Klhdc8b;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Heart;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Involved in pinching off the separated nuclei at the cleavage
CC       furrow and in cytokinesis. Required for mitotic integrity and
CC       maintenance of chromosomal stability. Protects cells against mitotic
CC       errors, centrosomal amplification, micronucleus formation and
CC       aneuploidy. Plays a key role of midbody function involving abscission
CC       of the daughter cells during cytokinesis and appropriate chromosomal
CC       and nuclear segregation into the daughter cells.
CC       {ECO:0000250|UniProtKB:Q8IXV7}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q8IXV7}. Midbody
CC       {ECO:0000250|UniProtKB:Q8IXV7}. Note=In mitotic cells, concentrates in
CC       the midbody of the cytoplasmic bridge linking daughter cells as they
CC       are about to separate during cytokinesis.
CC       {ECO:0000250|UniProtKB:Q8IXV7}.
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DR   EMBL; BC083777; AAH83777.1; -; mRNA.
DR   RefSeq; NP_001007686.1; NM_001007685.1.
DR   AlphaFoldDB; Q5XIA9; -.
DR   SMR; Q5XIA9; -.
DR   STRING; 10116.ENSRNOP00000066316; -.
DR   PaxDb; Q5XIA9; -.
DR   GeneID; 306589; -.
DR   KEGG; rno:306589; -.
DR   CTD; 200942; -.
DR   RGD; 1359445; Klhdc8b.
DR   VEuPathDB; HostDB:ENSRNOG00000047867; -.
DR   eggNOG; KOG1072; Eukaryota.
DR   HOGENOM; CLU_046864_0_0_1; -.
DR   InParanoid; Q5XIA9; -.
DR   OMA; TRFPCIP; -.
DR   OrthoDB; 933937at2759; -.
DR   PhylomeDB; Q5XIA9; -.
DR   PRO; PR:Q5XIA9; -.
DR   Proteomes; UP000002494; Chromosome 8.
DR   Bgee; ENSRNOG00000047867; Expressed in heart and 18 other tissues.
DR   Genevisible; Q5XIA9; RN.
DR   GO; GO:0110070; C:cellularization cleavage furrow; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0045171; C:intercellular bridge; ISS:UniProtKB.
DR   GO; GO:0030496; C:midbody; ISS:UniProtKB.
DR   GO; GO:1902410; P:mitotic cytokinetic process; ISS:UniProtKB.
DR   GO; GO:0140014; P:mitotic nuclear division; ISS:UniProtKB.
DR   GO; GO:0098813; P:nuclear chromosome segregation; ISS:UniProtKB.
DR   Gene3D; 2.120.10.80; -; 2.
DR   InterPro; IPR015915; Kelch-typ_b-propeller.
DR   InterPro; IPR006652; Kelch_1.
DR   Pfam; PF01344; Kelch_1; 4.
DR   SMART; SM00612; Kelch; 6.
DR   SUPFAM; SSF117281; SSF117281; 2.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Cytoplasm; Kelch repeat; Reference proteome;
KW   Repeat.
FT   CHAIN           1..354
FT                   /note="Kelch domain-containing protein 8B"
FT                   /id="PRO_0000119134"
FT   REPEAT          1..31
FT                   /note="Kelch 1"
FT   REPEAT          32..79
FT                   /note="Kelch 2"
FT   REPEAT          81..127
FT                   /note="Kelch 3"
FT   REPEAT          128..175
FT                   /note="Kelch 4"
FT   REPEAT          176..222
FT                   /note="Kelch 5"
FT   REPEAT          224..281
FT                   /note="Kelch 6"
FT   REPEAT          282..329
FT                   /note="Kelch 7"
FT   REPEAT          331..354
FT                   /note="Kelch 8"
SQ   SEQUENCE   354 AA;  37593 MW;  98A5EF3876ADA988 CRC64;
     MAAGGGQAFA WQVFPPMPTC RVYGTVAHQD GHLLVLGGCG RAGLPLDTAE TLDMASHTWL
     ALAPLPTARA GAAAVVLGKQ VLVVGGVDEV QSPVAAVEAF LADEGRWERR ATLPQAAMGV
     ATVERDGMVY ALGGMGPDTA PQAQVLVYES RRDRWLSLPS MPTPCYGAST FLHGNKIYVL
     GGRQGKLPVT AFEAFDLETR TWTRHPSLPS RRAFAGCAMA EGSVFSLGGL QQPGPHNFYS
     RPHFVNTVEM FDLEHGSWTK LPRSLRMRDK RADFVVGSLG GNIVAIGGLG NQPCPLASVE
     SFSLARRRWE ALPAMPTARC SCSSLQAGPR LFVIGGVAQG PSQAVEALCL RDGV