ID   KLDC2_RAT               Reviewed;         406 AA.
AC   Q3KRE6;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Kelch domain-containing protein 2 {ECO:0000305};
GN   Name=Klhdc2 {ECO:0000312|RGD:1306504};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Prostate;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Substrate-recognition component of a Cul2-RING (CRL2) E3
CC       ubiquitin-protein ligase complex of the DesCEND (destruction via C-end
CC       degrons) pathway, which recognizes a C-degron located at the extreme C
CC       terminus of target proteins, leading to their ubiquitination and
CC       degradation. The C-degron recognized by the DesCEND pathway is usually
CC       a motif of less than ten residues and can be present in full-length
CC       proteins, truncated proteins or proteolytically cleaved forms. The
CC       CRL2(KLHDC2) complex specifically recognizes proteins with a diglycine
CC       (Gly-Gly) at the C-terminus, leading to their ubiquitination and
CC       degradation. The CRL2(KLHDC2) complex mediates ubiquitination and
CC       degradation of truncated SELENOK and SELENOS selenoproteins produced by
CC       failed UGA/Sec decoding, which end with a diglycine. The CRL2(KLHDC2)
CC       complex also recognizes proteolytically cleaved proteins ending with
CC       Gly-Gly, such as the N-terminal fragment of USP1, leading to their
CC       degradation. May also act as an indirect repressor of CREB3-mediated
CC       transcription by interfering with CREB3-DNA-binding.
CC       {ECO:0000250|UniProtKB:Q9Y2U9}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000250|UniProtKB:Q9Y2U9}.
CC   -!- SUBUNIT: Component of a CRL2 E3 ubiquitin-protein ligase complex, also
CC       named ECS (Elongin BC-CUL2/5-SOCS-box protein) complex, composed of
CC       CUL2, Elongin BC (ELOB and ELOC), RBX1 and substrate-specific adapter
CC       KLHDC2. Interacts with CREB3; interaction is direct and specific as it
CC       does not interact with CREB1, ATF4, ATF6, JUN, FOS, CEBPA or herpes
CC       simplex virus transactivator VP16. {ECO:0000250|UniProtKB:Q9Y2U9}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9Y2U9}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC105756; AAI05757.1; -; mRNA.
DR   RefSeq; NP_001029305.1; NM_001034133.1.
DR   AlphaFoldDB; Q3KRE6; -.
DR   SMR; Q3KRE6; -.
DR   STRING; 10116.ENSRNOP00000006223; -.
DR   PaxDb; Q3KRE6; -.
DR   PRIDE; Q3KRE6; -.
DR   Ensembl; ENSRNOT00000006223; ENSRNOP00000006223; ENSRNOG00000004474.
DR   GeneID; 299113; -.
DR   KEGG; rno:299113; -.
DR   UCSC; RGD:1306504; rat.
DR   CTD; 23588; -.
DR   RGD; 1306504; Klhdc2.
DR   eggNOG; KOG0379; Eukaryota.
DR   GeneTree; ENSGT00940000157150; -.
DR   HOGENOM; CLU_042804_0_0_1; -.
DR   InParanoid; Q3KRE6; -.
DR   OMA; YNDGNCH; -.
DR   OrthoDB; 1263405at2759; -.
DR   PhylomeDB; Q3KRE6; -.
DR   TreeFam; TF314081; -.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q3KRE6; -.
DR   Proteomes; UP000002494; Chromosome 6.
DR   Bgee; ENSRNOG00000004474; Expressed in cerebellum and 19 other tissues.
DR   Genevisible; Q3KRE6; RN.
DR   GO; GO:0031462; C:Cul2-RING ubiquitin ligase complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; ISS:UniProtKB.
DR   GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   GO; GO:0140627; P:ubiquitin-dependent protein catabolic process via the C-end degron rule pathway; ISS:UniProtKB.
DR   Gene3D; 2.120.10.80; -; 2.
DR   InterPro; IPR015915; Kelch-typ_b-propeller.
DR   SUPFAM; SSF117281; SSF117281; 2.
PE   2: Evidence at transcript level;
KW   Kelch repeat; Nucleus; Reference proteome; Repeat; Ubl conjugation pathway.
FT   CHAIN           1..406
FT                   /note="Kelch domain-containing protein 2"
FT                   /id="PRO_0000228994"
FT   REPEAT          43..89
FT                   /note="Kelch 1"
FT   REPEAT          102..155
FT                   /note="Kelch 2"
FT   REPEAT          157..202
FT                   /note="Kelch 3"
FT   REPEAT          229..278
FT                   /note="Kelch 4"
FT   REPEAT          280..329
FT                   /note="Kelch 5"
FT   REPEAT          331..377
FT                   /note="Kelch 6"
SQ   SEQUENCE   406 AA;  45926 MW;  F06F8452B63BE0D1 CRC64;
     MADGNEDARA EDMPGPAFEG YEAMELACPA ERSGHVAVSD GRHMFVWGGY KSNQVRGLYD
     FYLPREELWI YNMETGRWKK INTEGDVPPS MSGSCAVCVD RVLYLFGGHH SRGNTNKFYM
     LDSRSADRVL QWERIDCQGI PPSSKDKLGV WVYKNKLIFF GGYGYLPEDK VLGTFEFDET
     SFWNSSHPRG WNDHVHILDT ETFAWSQPIT TGKAPSPRAA HACATVGNKG FVFGGRYRDA
     RMNDLHYLNL DTWEWNELIP QGICPVGRSW HSLTPVSSDH LFLFGGFTTE KQPLSDAWTY
     CISKNEWIQF NHPYVEKPRL WHTACASDEG EVIVFGGCAN NLLVHHRAAH SNEVLIFSVQ
     PKSLVRLSLE AVICFKEMLA NSWSCLPKHL LHSVNQRFGS NNTSGS