KLDC3_BOVIN
ID KLDC3_BOVIN Reviewed; 382 AA.
AC Q58CV6; Q5EAB3;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2007, sequence version 2.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Kelch domain-containing protein 3 {ECO:0000250|UniProtKB:Q9BQ90};
GN Name=KLHDC3 {ECO:0000250|UniProtKB:Q9BQ90};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RX PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT "Characterization of 954 bovine full-CDS cDNA sequences.";
RL BMC Genomics 6:166-166(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=Hereford; TISSUE=Thymus;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Substrate-recognition component of a Cul2-RING (CRL2) E3
CC ubiquitin-protein ligase complex of the DesCEND (destruction via C-end
CC degrons) pathway, which recognizes a C-degron located at the extreme C
CC terminus of target proteins, leading to their ubiquitination and
CC degradation. The C-degron recognized by the DesCEND pathway is usually
CC a motif of less than ten residues and can be present in full-length
CC proteins, truncated proteins or proteolytically cleaved forms. The
CC CRL2(KLHDC3) complex specifically recognizes proteins with a glycine
CC (Gly) at the C-terminus, leading to their ubiquitination and
CC degradation: recognizes the C-terminal -Arg-(Xaa)n-Arg-Gly, -Arg-
CC (Xaa)n-Lys-Gly, and -Arg-(Xaa)n-Gln-Gly degrons. The CRL2(KLHDC3)
CC complex mediates ubiquitination and degradation of truncated SELENOV
CC and SEPHS2 selenoproteins produced by failed UGA/Sec decoding, which
CC end with a glycine. May be involved in meiotic recombination process.
CC {ECO:0000250|UniProtKB:Q9BQ90}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000250|UniProtKB:Q9BQ90}.
CC -!- SUBUNIT: Component of a CRL2 E3 ubiquitin-protein ligase complex, also
CC named ECS (Elongin BC-CUL2/5-SOCS-box protein) complex, composed of
CC CUL2, Elongin BC (ELOB and ELOC), RBX1 and substrate-specific adapter
CC KLHDC3. {ECO:0000250|UniProtKB:Q9BQ90}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9BQ90}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q58CV6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q58CV6-2; Sequence=VSP_024354;
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DR EMBL; BT020656; AAX08673.1; -; mRNA.
DR EMBL; BT021774; AAX46621.1; -; mRNA.
DR EMBL; BT021841; AAX46688.1; -; mRNA.
DR EMBL; BC103002; AAI03003.1; -; mRNA.
DR RefSeq; NP_001014966.1; NM_001014966.1. [Q58CV6-1]
DR AlphaFoldDB; Q58CV6; -.
DR SMR; Q58CV6; -.
DR STRING; 9913.ENSBTAP00000016944; -.
DR PRIDE; Q58CV6; -.
DR Ensembl; ENSBTAT00000016943; ENSBTAP00000016943; ENSBTAG00000050696. [Q58CV6-1]
DR GeneID; 541138; -.
DR KEGG; bta:541138; -.
DR CTD; 116138; -.
DR VEuPathDB; HostDB:ENSBTAG00000050696; -.
DR eggNOG; KOG3190; Eukaryota.
DR eggNOG; KOG4693; Eukaryota.
DR GeneTree; ENSGT00940000157952; -.
DR HOGENOM; CLU_045453_0_0_1; -.
DR InParanoid; Q58CV6; -.
DR OMA; AYEQERM; -.
DR OrthoDB; 933937at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000009136; Chromosome 23.
DR Bgee; ENSBTAG00000050696; Expressed in spermatid and 106 other tissues.
DR ExpressionAtlas; Q58CV6; baseline and differential.
DR GO; GO:0031462; C:Cul2-RING ubiquitin ligase complex; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; ISS:UniProtKB.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.120.10.80; -; 2.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR InterPro; IPR011498; Kelch_2.
DR Pfam; PF01344; Kelch_1; 1.
DR Pfam; PF07646; Kelch_2; 1.
DR SUPFAM; SSF117281; SSF117281; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Kelch repeat; Meiosis; Reference proteome;
KW Repeat; Ubl conjugation pathway.
FT CHAIN 1..382
FT /note="Kelch domain-containing protein 3"
FT /id="PRO_0000237571"
FT REPEAT 25..77
FT /note="Kelch 1"
FT REPEAT 88..138
FT /note="Kelch 2"
FT REPEAT 139..189
FT /note="Kelch 3"
FT REPEAT 191..249
FT /note="Kelch 4"
FT REPEAT 251..301
FT /note="Kelch 5"
FT VAR_SEQ 362..382
FT /note="WELNAMTTNSNISRPIVSSHG -> YSEWLEGRD (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16305752"
FT /id="VSP_024354"
SQ SEQUENCE 382 AA; 43058 MW; FCA0DADCB9294F08 CRC64;
MLRWTVHLEG GPRRVNHAAV AVGHRVYSFG GYCSGEDYET LRQIDVHIFN AVSLRWTKLP
PVRPAARGQA PVVPYMRYGH STVLIDDTVF LWGGRNDTEG ACNVLYAFDV NTHKWSTPRV
SGTVPGARDG HSACVLGKTM YIFGGYEQLA DCFSNDIHKL DTSTMTWTLI CTKGNPARWR
DFHSATMLGS HMYVFGGRAD RFGPFHSNNE IYCNRIRVFD TRTEAWLDCP PTPVLPEGRR
SHSAFGYNGE LYIFGGYNAR LNRHFHDLWK FNPVSFTWKK IEPKGKGPCP RRRQCCCIVG
DKIVLFGGTS PSPEEGLGDE FDLIDHSDLH ILDFSPSLKT LCKLAVIQYN LDQSCLPHDI
RWELNAMTTN SNISRPIVSS HG
